Human Lipocalin-2/NGAL ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Lipocalin-2/NGAL ELISA antibody pairs, Human, for 5 plates

Human Lipocalin-2/NGAL ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human LCN2 in cell culture supernatants, serum, plasma (heparin) , saliva and urine. Cited in 5 publication(s).

Product Info Summary

SKU: EZ0853
Size: 5 plates/kit
Reactive Species: Human
Application: ELISA

Product Name

Human Lipocalin-2/NGAL ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all Lipocalin-2/NGAL products

SKU/Catalog Number

EZ0853

Size

5 plates/kit

*Question: How many samples can I assay/run in this kit?

Description

Human Lipocalin-2/NGAL ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human LCN2 in cell culture supernatants, serum, plasma (heparin) , saliva and urine.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human Lipocalin-2/NGAL ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0853)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q21-G198

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0853 is reactive to LCN2 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin), saliva and urine.

Application Guarantee

EZ0853 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Storage of opened/reconstituted material
EZ0853-CA Rabbit anti- human LCN2 polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit.
EZ0853-DA Biotinylated goat anti- human LCN2 polyclonal antibody (Detection Antibody) 500 µl, 4.7 µg/mL (recommended dilution 1:100)
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)
EK853-ST Lyophilized recombinant human LCN2 standard 10 ng/tube×3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Incubator.
  • 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 5. Clean tubes and Eppendorf tubes.
  • 6. 96 well microplate (Cat# AR1100)
  • 7. Plate Sealers.
  • 8. Capture Antibody Diluent: PBS.
  • 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 11. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 12. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.040.3170.4790.781.0491.5881.8772.09

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2561510542225415375138
Standard deviation19.281.54281.9419.0584.53323.69
CV (%)7.5%5.4%5.2%7.5%5.5%6.3%

Gene/Protein Information For LCN2 (Source: Uniprot.Org, NCBI)

Gene Name

LCN2

Full Name

Neutrophil gelatinase-associated lipocalin

Weight

22.588kDa

Superfamily

calycin superfamily

Alternative Names

24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin LCN2 24p3, MSFI, NGAL, p25 lipocalin 2 neutrophil gelatinase-associated lipocalin|25 kDa alpha-2-microglobulin-related subunit of MMP-9|migration-stimulating factor inhibitor|oncogene 24p3|siderocalin LCN2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on LCN2, check out the LCN2 Infographic

LCN2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for LCN2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EZ0853 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Gelatinases and their tissue inhibitors in a group of subjects with obstructive sleep apnea syndrome

Endothelial Glycocalyx Damage Is Associated with Leptospirosis Acute Kidney Injury

Effect of Omega-3 Supplementation on Lipocalin 2 and Retinol-Binding Protein 4 in Type 2 Diabetic Patients

Urine and serum neutrophil gelatinase-associated lipocalin cut-off point for the prediction of acute kidney injury

Guo Sx, Fang Q, You Cg, Jin Yy, Wang Xg, Hu Xl, Han Cm. J Transl Med. 2015 Jun 6;13:183. Doi: 10.1186/S12967-015-0548-3. Effects Of Hydrogen-Rich Saline On Early Acute Kidney Injury In Severely Burned Rats By Suppressing Oxidative Stress Induced A...

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