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Product Info Summary
| SKU: | EK0776 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human IGF1R/Igf1 Receptor ELISA Kit PicoKine®
View all IGF-I R/IGF1R ELISA kits
SKU/Catalog Number
EK0776
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IGF1R/Igf1 Receptor ELISA Kit PicoKine™ (96 Tests). Quantitate Human IGF1R in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IGF1R/Igf1 Receptor ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0776)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: E31-N932
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0776 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0776 is reactive to IGF1R in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0776 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IGF-I R/IGF1R
IGF1R, also called IGFR or insulin-like growth factor 1 receptor, is a protein that found on the surface of human cells. This gene is mapped to 15q26.3. IGF1R belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in growth and continues to have anabolic effects in adults - meaning that it can induce hypertrophy of skeletal muscle and other target tissues. IGF1R also plays a critical role in transformation events. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0776-CAP | Anti-Human IGF1R Pre-coated 96-well strip microplate | 1 |
| EK0776-ST | Human IGF1R Standard | 2 vials, 10 ng/tube |
| EK0776-DA | Human IGF1R Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.031 | 0.112 | 0.170 | 0.291 | 0.469 | 0.823 | 1.376 | 2.027 |
Data Example Images
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Human IGF1R PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IGF-I R/IGF1R, Dilution ratio of 1:1, concentration in serum and plasma is around 500 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IGF1R/Igf1 Receptor ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 332 | 1874 | 5843 | 363 | 1924 | 5918 |
| Standard deviation | 17.92 | 89.95 | 437.02 | 25.41 | 121.21 | 526.7 |
| CV (%) | 5.4% | 4.8% | 7.5% | 7% | 6.3% | 8.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IGF-I R/IGF1R in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 332 | 335 | 290 | 335 | 323 | 19.09 | 5.9% |
| Sample 2 | 1874 | 1833 | 1855 | 2088 | 1912 | 102.35 | 5.3% |
| Sample 3 | 5843 | 5933 | 6248 | 5526 | 5887 | 257.24 | 4.3% |
Protein Target Info & Infographic
Gene/Protein Information For IGF1R (Source: Uniprot.Org, NCBI)
Gene Name
IGF1R
Full Name
Insulin-like growth factor 1 receptor
Weight
154.793kDa
Superfamily
protein kinase superfamily
Alternative Names
CD221 antigen; CD221; EC 2.7.10; EC 2.7.10.1; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; Insulin-like growth factor I receptor; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2 IGF1R CD221, IGFIR, IGFR, JTK13 insulin like growth factor 1 receptor insulin-like growth factor 1 receptor|IGF-I receptor|soluble IGF1R variant 1|soluble IGF1R variant 2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IGF1R, check out the IGF1R Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IGF1R: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human IGF1R/Igf1 Receptor ELISA Kit PicoKine® (EK0776)
Hello CJ!
EK0776 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Alashti, M.,Shirvani, H.,Sabzevari Rad,R. et al.Effects of different small-sided games on blood lactate and GH/IGF-1 axis responses in young soccer players.Ger J Exerc Sport Res(2020).https://doi.org/10.1007/s1266 2-020-00691-6
Species: EK0578;APP:ELISA;SAMPLE:BLOOD;DILUTION:NA|EK0776;APP:ELISA;SAMPLE:BLOOD;DILUTION:NA
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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9 Customer Q&As for Human IGF1R/Igf1 Receptor ELISA Kit PicoKine®
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-08-10
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-08-10
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
G. Jones
Verified customer
Asked: 2020-05-30
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-05-30
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement IGF-I R using the EK0776 Human IGF-I R Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-01-04
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2019-01-04
Question
Q: how to thaw whole blood sample for IGF-I R ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-10-24
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IGF-I R for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-10-24
Question
Q: Can IGF-I R ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2018-08-04
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-08-04
Question
Q: is it okay to use citrate plasma as samples in Human IGF-I R Picokine® ELISA Kit (Catalog # EK0776)?
Verified Customer
Verified customer
Asked: 2018-07-10
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of IGF-I R causing disruption of its protein structure. IGF-I R may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IGF-I R ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-07-10
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
D. Zhou
Verified customer
Asked: 2017-10-20
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2017-10-20
Question
To Boster; I have queries about EK0776 Human IGF1R/Igf1 Receptor ELISA Kit. Is the kit tested on rat samples? If not, do the capture and detection antibodies have been tested on rat samples individually? Also is EK0776 Human IGF1R/Igf1 Receptor ELISA Kit predicted to react with rat IGF1R based on homology of the rat protein with antibody immunogen sequence?
Verified Customer
Verified customer
Asked: 2017-09-15
Answer
Thank you for these queries, the EK0776 Human IGF1R/Igf1 Receptor ELISA Kit had not been tested on rat samples nor had the antibodies individually. Though the immunogen used to generate the antibodies is 96% identical to the homologous rat IGFR1 amino acid sequence. Therefore, there is a strong likelihood of cross-reaction.
Boster Scientific Support
Answered: 2017-09-15
Question
Q: how do I analyze ELISA data? I have obtained IGF-I R level in plasma.
P. Wang
Verified customer
Asked: 2015-06-17
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2015-06-17
