Human TIMP-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

Human TIMP-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human TIMP4 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10 pg/ml.

Product Info Summary

SKU: EZ0524
Size: For 5 plates, 96 wells each
Reactive Species: Human
Application: ELISA

Product Name

Human TIMP-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs)

See all TIMP-4 products

SKU/Catalog Number

EZ0524

Size

For 5 plates, 96 wells each

*Question: How many samples can I assay/run in this kit?

Description

Human TIMP-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (antibody pairs and standards for assay development). Quantitate Human TIMP4 in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human TIMP-4 ELISA Kit EZ-Set™ (DIY Antibody Pairs) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EZ0524)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: sf21; Immunogen sequence: C30-P224

Sensitivity

<10 pg/ml

Assay Range

78 pg/ml - 5,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EZ0524 is reactive to TIMP4 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.

Application Guarantee

EZ0524 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog number Description Quantity Buffers
EZ0524-CA Rabbit anti- human TIMP4 polyclonal antibody (Capture Antibody) 500 µl, 0.4 mg/mL (recommended dilution 1:100) 0.5% Na2HPO4, 0.04% Proclin 300 and 50% Glycerol
EZ0524-DA Biotinylated goat anti- human TIMP4 polyclonal antibody (Detection Antibody) 500 µl, 2.5 µg/mL (recommended dilution 1:100) 0.04% Proclin 300, 0.5% BSA, 0.2% Tris, 1% NaCl and 30% Glycerol
EZ0524-ST Lyophilized recombinant human TIMP4 standard 10 ng/tube×2
AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 µl (recommended dilution 1:100)

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • 1. Microplate reader in standard size.
  • 2. Automated plate washer.
  • 3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • 4. Clean tubes and Eppendorf tubes.
  • 5. 96 well microplate (Cat# AR1100)
  • 6. Plate Sealers.
  • 7. Capture Antibody Diluent: PBS.
  • 8. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 µm filtered.
  • 9. Substrate Solution: Tetramethylbenzidine (Cat# AR1104)
  • 10. Stop Solution: 2 N H2SO4 (Cat# AR1105)
  • 11. Wash Buffer (PBS and PBS-T). Recipes:
    PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 µm filtered.
    PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4.
  • *Item 5 – 11 are included in the EZ Set Accessory Kit (EZA001)

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)078156313625125025005000
O.D.0.0540.140.2060.3750.661.1271.8432.229

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)12358920561226011963
Standard deviation9.4738.87125.4110.9848.08155.07
CV (%)7.7%6.6%6.1%9%8%7.9%

Gene/Protein Information For TIMP4 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q99727

Gene ID

7079

Gene Name

TIMP4

Full Name

Metalloproteinase inhibitor 4

Weight

25.503kDa

Superfamily

protease inhibitor I35 (TIMP) family

Alternative Names

metalloproteinase inhibitor 4; TIMP metallopeptidase inhibitor 4; TIMP4; TIMP-4; tissue inhibitor of metalloproteinase 4; Tissue inhibitor of metalloproteinases 4 TIMP4 TIMP-4 TIMP metallopeptidase inhibitor 4 metalloproteinase inhibitor 4|tissue inhibitor of metalloproteinase 4|tissue inhibitor of metalloproteinases 4

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TIMP4, check out the TIMP4 Infographic

TIMP4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TIMP4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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