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Product Info Summary
| SKU: | EK0568 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and serum. |
Product info
Product Name
Mouse MCP-1 ELISA Kit PicoKine®
SKU/Catalog Number
EK0568
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse MCP-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ccl2 in cell culture supernatants and serum. Sensitivity: 6pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse MCP-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0568)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: Q24-R96
Sensitivity
<6 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0568 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0568 is reactive to CCL2 in Mouse samples
Validated Sample Types
cell culture supernatants and serum.
Application Guarantee
EK0568 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCL2
Monocyte chemotactic protein-1 (MCP-1) is a member of the small inducible gene (SIG) family. It has been shown to play a role in the recruitment of monocytes to sites of injury and infection. By analysis of a panel of somatic cell hybrids, we have localized the MCP-1 gene, designated SCYA2, to human chromosome 17. In situ hybridization confirmed this assignment and further localized the gene to 17q11.2-q21.1. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Monocyte chemoattractant protein-1 (MCP-1), regulated on activation, normal T cell expressed and secreted,and stimulation of monocytes from healthy carriers of the genotype GG with Mycobacterium tuberculosis antigens yielded higher MCP-1. The standard used in this kit is recombinant mouse MCP-1 (Q24-R96) with the molecular mass of 8.5Kda.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0568-CAP | Anti-Mouse CCL2 Pre-coated 96-well strip microplate | 1 |
| EK0568-ST | Mouse CCL2 Standard | 2 vials, 10 ng/tube |
| EK0568-DA | Mouse CCL2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.014 | 0.051 | 0.090 | 0.193 | 0.406 | 0.726 | 1.465 | 1.897 |
Data Example Images
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Mouse MCP-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CCL2, Dilution ratio of 1:1, concentration in serum is 51-259 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse MCP-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 39 | 224 | 394 | 40 | 231 | 398 |
| Standard deviation | 1.56 | 10.75 | 27.18 | 2 | 12.47 | 27.46 |
| CV (%) | 4% | 4.8% | 6.9% | 5% | 5.4% | 6.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CCL2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 39 | 36 | 34 | 34 | 35 | 2.04 | 5.8% |
| Sample 2 | 224 | 228 | 228 | 219 | 224 | 3.69 | 1.6% |
| Sample 3 | 394 | 375 | 431 | 394 | 398 | 20.3 | 5.1% |
Protein Target Info & Infographic
Gene/Protein Information For CCL2 (Source: Uniprot.Org, NCBI)
Gene Name
CCL2
Full Name
C-C motif chemokine 2
Weight
11.025kDa
Superfamily
intercrine beta (chemokine CC) family
Alternative Names
C-C motif chemokine ligand 2; CCL2; GDCF-2; HC11; HSMCR30; MCAF; Mcp1; MCP-1; SCYA2; SMC-CF CCL2 GDCF-2, HC11, HSMCR30, MCAF, MCP-1, MCP1, SCYA2, SMC-CF C-C motif chemokine ligand 2 C-C motif chemokine 2|chemokine (C-C motif) ligand 2|monocyte chemoattractant protein-1|monocyte chemotactic and activating factor|monocyte chemotactic protein 1|monocyte secretory protein JE|small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je)|small inducible cytokine subfamily A (Cys-Cys), member 2|small-inducible cytokine A2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CCL2, check out the CCL2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CCL2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse MCP-1 ELISA Kit PicoKine® (EK0568)
Hello CJ!
EK0568 has been cited in 73 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Involvement of TLR4 in Oxidized LDL/β2GPI/Anti-β2GPI-Induced Transformation of Macrophages to Foam Cells
USP19 Inhibits TNF-α– and IL-1β–Triggered NF-κB Activation by Deubiquitinating TAK1
The Extra-Virgin Olive Oil Polyphenols Oleocanthal and Oleacein Counteract Inflammation-Related Gene and miRNA Expression in Adipocytes by Attenuating NF-κB Activation
Chlorogenic Acid-Induced Gut Microbiota Improves Metabolic Endotoxemia
The effects of Panax notoginseng saponins on the cytokines and peritoneal function in rats with peritoneal fibrosis
Role of dysfunctional adipocytes in cholesterol-induced nonobese metabolic syndrome
Protective Effect of Irisin on Atherosclerosis via Suppressing Oxidized Low Density Lipoprotein Induced Vascular Inflammation and Endothelial Dysfunction
Inhibition of inflammation using diacerein markedly improved renal function in endotoxemic acute kidney injured mice
RIPK3 Induces Cardiomyocyte Necroptosis via Inhibition of AMPK-Parkin-Mitophagy in Cardiac Remodelling after Myocardial Infarction
Shexiang Baoxin Pill Alleviates the Atherosclerotic Lesions in Mice via Improving Inflammation Response and Inhibiting Lipid Accumulation in the Arterial Wall
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Customer Reviews
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1 Reviews For Mouse MCP-1 ELISA Kit PicoKine®
0
Good reproducible estimation--Divya Sharma, NDSU, Pharmaceutical Sciences, Graduate Student
Excellent
Source: Biocompare.com
| Application | ELISA |
|---|---|
| Sample | Centrifuged cell culture media supernatant |
| Detection | -- |
"Had to perform the analysis twice initially as the background wasn't clear. Overall, good reproducible estimation. Reliable, reproducible. Good product but would appreciate a faster assay kit with less chances of manual error." (Customer’s Tip: "Perform washing steps at least 6-7 times to get a clearer background with 60 seconds wait period in between each washing.")
Divya Sharma
Verified customer
Submitted 2019-05-21
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
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Submit your question
7 Customer Q&As for Mouse MCP-1 ELISA Kit PicoKine®
Question
Q: how do I analyze ELISA data? I have performed CCL2 level in serum.
Verified Customer
Verified customer
Asked: 2020-08-07
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-08-07
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-06-01
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-06-01
Question
Can you confirm if EK0394,EK0568,EK0527,EK0411 and EK0596 are compatible with ELISA microplate washers?
Verified customer
Asked: 2019-05-30
Answer
Our ELISA kits should be compatible for all ELISA microplate washers as long as it supports a 96 well plate.
Boster Scientific Support
Answered: 2019-05-30
Question
Can the sample diluent buffer, ABC diluent buffer and antibody diluent buffer be used interchangeably between the kit?
Verified customer
Asked: 2019-05-28
Answer
For the Mouse IL-1 Beta ELISA Kit PicoKine™ (EK0394), Mouse MCP-1 ELISA Kit PicoKine™ (EK0568), Mouse TNF Alpha ELISA Kit PicoKine™ (EK0527), Mouse IL-6 ELISA Kit PicoKine™ (EK0411) and Mouse Adiponectin ELISA Kit PicoKine™ (EK0596), the sample diluent buffer, ABC diluent buffer and antibody diluent buffer can be used interchangeably between the kit. However, the sample diluent buffer can only be used to dilute standards and samples, ABC diluent buffer is used to dilute ABC only and antibody diluent buffer is used to dilute antibody only.
Boster Scientific Support
Answered: 2019-05-29
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
W. Wright
Verified customer
Asked: 2017-08-30
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-08-30
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
C. Young
Verified customer
Asked: 2014-08-26
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2014-08-26
Question
Q: if the enzyme conjugated CCL2 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CCL2 antigen?
Verified Customer
Verified customer
Asked: 2014-05-22
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2014-05-22
