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Product Info Summary
| SKU: | EK0874 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine®
View all CD31/PECAM-1 ELISA kits
SKU/Catalog Number
EK0874
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Pecam1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0874)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: E18-K590
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0874 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0874 is reactive to PECAM1 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0874 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD31/PECAM-1
PECAM-1 is an important target GP in DITP.1 Platelet/endothelial cell adhesion molecule-1 (PECAM-1) is a 130-kD member of the Ig gene superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and selective T-cell subsets.2 Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) is a member of the immunoglobulin (Ig) superfamily that has distinctive features of an immunoreceptor based upon its genomic structure and the presence of intrinsic immunoreceptor tyrosine inhibitory motifs (ITIMs) in its ligand binding polypeptide.3 PECAM-1 has recently been shown to contain functional immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within its cytoplasmic domain, and co-ligation of PECAM-1 with the T-cell antigen receptor (TCR) results in tyrosine phosphorylation of PECAM-1, recruitment of Src homology 2 domain-containing protein tyrosine phosphatase-2 (SHP-2), and attenuation of TCR-mediated cellular signaling.4 The standard used in this kit is the product of gene recombinant expression (extracellular part), consisting of 563 amino acids (E28-K590) with the molecular weight of 65.5KDa. After glycosylating, its molecular weight changes to 91-100KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0874-CAP | Anti-Mouse PECAM1 Pre-coated 96-well strip microplate | 1 |
| EK0874-ST | Mouse PECAM1 Standard | 2 vials, 10 ng/tube |
| EK0874-DA | Mouse PECAM1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 | 10,000 |
| O.D. | 0.032 | 0.078 | 0.149 | 0.245 | 0.413 | 0.751 | 1.246 | 1.952 |
Data Example Images
Click image to see more details
Mouse PECAM-1/CD31 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CD31/PECAM-1, Dilution ratio of 1:20, concentration in serum and plasma is 27.5-48.2 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 228 | 1326 | 5663 | 230 | 1398 | 5316 |
| Standard deviation | 17.32 | 74.25 | 407.73 | 18.17 | 99.25 | 409.33 |
| CV (%) | 7.6% | 5.6% | 7.2% | 7.9% | 7.1% | 7.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD31/PECAM-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 228 | 217 | 221 | 199 | 216 | 10.7 | 4.9% |
| Sample 2 | 1326 | 1359 | 1160 | 1320 | 1291 | 77.21 | 5.9% |
| Sample 3 | 5663 | 6219 | 5814 | 5340 | 5759 | 315.99 | 5.4% |
Protein Target Info & Infographic
Gene/Protein Information For PECAM1 (Source: Uniprot.Org, NCBI)
Gene Name
PECAM1
Full Name
Platelet endothelial cell adhesion molecule
Weight
82.522kDa
Alternative Names
adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule PECAM1 CD31, CD31/EndoCAM, GPIIA, PECA1, PECAM-1, endoCAM platelet and endothelial cell adhesion molecule 1 platelet endothelial cell adhesion molecule|CD31 antigen|platelet endothelial cell adhesion molecule-1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on PECAM1, check out the PECAM1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for PECAM1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine® (EK0874)
Hello CJ!
EK0874 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yang S, Zhu X, Cai L, Cheng H, Zhao R, Wang H, Zhao H, Wang Z. Cancer Sci. 2010 Mar;101(3):679-85. Doi: 10.1111/J.1349-7006.2009.01436.X. Epub 2009 Nov 14. Role Of Tumor-Associated Lymphatic Endothelial Cells In Metastasis: A Study Of Epithelial O...
Wang Tb, Wei Xq, Lin Wh, Shi Hp, Dong Wg. Clin Exp Med. 2012 Jun;12(2):89-95. Doi: 10.1007/S10238-011-0143-8. Epub 2011 Jun 10. The Inhibition Of Endostar On The Angiogenesis And Growth Of Gastrointestinal Stromal Tumor Xenograft.
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Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
L. Hernandez
Verified customer
Asked: 2020-09-08
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-09-08
Question
Q: What is the optimal O.D. value for CD31 ELISA kit? I used your CD31 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CD31 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2020-08-01
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD31 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CD31 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-08-01
Question
Q: how to thaw whole blood sample for CD31 ELISA after freezing?
H. Green
Verified customer
Asked: 2019-05-06
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD31 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-05-06
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
M. Moore
Verified customer
Asked: 2019-03-11
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-03-11
Question
Q: if the enzyme conjugated CD31 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD31 antigen?
Verified Customer
Verified customer
Asked: 2018-08-19
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-08-19
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
M. Wang
Verified customer
Asked: 2014-03-24
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2014-03-24
