NF-kappaB p100/p52 Colorimetric Cell-Based ELISA Kit

NFKB2 ELISA kit, cell-based

The NF-kappaB p100/p52 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor NF-kappaB p100/p52 protein expression profile in cells. The kit can be used for measuring the relative amounts of NF-kappaB p100/p52 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on NF-kappaB p100/p52.

Product Info Summary

SKU: EKC1400
Size: 1 kit, containing one 96-well plate and all necessary reagents
Reactive Species: Human, Mouse, Rat
Application: ELISA

Product Name

NF-kappaB p100/p52 Colorimetric Cell-Based ELISA Kit

See all NFKB2 products

SKU/Catalog Number

EKC1400

Size

1 kit, containing one 96-well plate and all necessary reagents

Description

The NF-kappaB p100/p52 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor NF-kappaB p100/p52 protein expression profile in cells. The kit can be used for measuring the relative amounts of NF-kappaB p100/p52 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on NF-kappaB p100/p52.

Storage & Handling

Store at 4°C for up to 6 months.

Cite This Product

NF-kappaB p100/p52 Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1400)

Clonality of Antibodies

See Datasheet for details

Assay Range

> 5000 cells/well

Reactive Species

EKC1400 is reactive to NFKB2 in Human, Mouse, Rat samples

Application Guarantee

EKC1400 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Reagent Quantity Container
96-Well Cell Culture Clear-Bottom Microplate 1 Plates -
10x TBS 24 ml (10x) Clear
Quenching Buffer 24 ml (1x) Clear
Blocking Buffer 50 ml (1x) Clear
15x Wash Buffer 50 ml (15x) Clear
100x Anti-NFKB2 Antibody (Rabbit Polyclonal) 60 µl (100x) Purple
100x Anti-GAPDH Antibody (Mouse Monoclonal) 60 µl (100x) Green
HRP-Conjugated Anti-Rabbit IgG Antibody 6ml (1x) Glass
HRP-Conjugated Anti-Mouse IgG Antibody 6ml (1x) Glass
Primary Antibody Diluent 12 ml (1x) Clear
Ready-to-Use Substrate 12 ml (1x) Brown
Stop Solution 12 ml (1x) Clear
Crystal Violet Solution 6ml (1x) Glass
SDS Solution 24 ml (1x) Clear
Adhesive Plate Seals 2 Seals -

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
  • Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
  • 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
  • Deionized or sterile water
  • Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
  • Graph paper or computer software capable of generating or displaying logarithmic functions
  • Absorbent papers or vacuum aspirator
  • Test tubes or microfuge tubes capable of storing 1 ml
  • Orbital shaker
  • Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)

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Gene/Protein Information For NFKB2 (Source: Uniprot.Org, NCBI)

Gene Name

NFKB2

Full Name

Nuclear factor NF-kappa-B p100 subunit

Weight

96.749kDa

Alternative Names

LYT10; NFkB2; NF-kB2 NFKB2 CVID10, H2TF1, LYT-10, LYT10, NF-kB2, p100, p49/p100, p52 nuclear factor kappa B subunit 2 nuclear factor NF-kappa-B p100 subunit|DNA-binding factor KBF2|NFKB, p52/p100 subunit|lymphocyte translocation chromosome 10 protein|nuclear factor Kappa-B, subunit 2|nuclear factor NF-kappa-B p52 subunit|nuclear factor of Kappa light chain gene enhancer in B cells 2|nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)|oncogene Lyt-10|transcription factor NFKB2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on NFKB2, check out the NFKB2 Infographic

NFKB2 infographic

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In this infographic you will see the following information for NFKB2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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1 Customer Q&As for NF-kappaB p100/p52 Colorimetric Cell-Based ELISA Kit

Question

Does EKC1400 measure both p100 and p52 protein levels? If so, p100 is processed as p52 when the such as Activator (LIGHT) is processed. In this case, is it possible to measure the change by LIGHT?

Verified customer

Asked: 2022-05-18

Answer

The NF-KappaB P100/P52 Colorimetric Cell-Based ELISA Kit (EKC1400) is predicted to detect the p100 subunit. It is not predicted to detect the p52 subunit.

Boster Scientific Support

Answered: 2022-05-18

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