Rat Lipocalin-2/NGAL PicoKine® Quick ELISA Kit

Lipocalin-2/NGAL ELISA kit, 90-minute one-step protocol

Rat Lipocalin-2/NGAL PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Lcn2 in cell culture supernatants, serum, plasma (heparin) and urine. Sensitivity: 10pg/ml.

Product Info Summary

SKU: FEK0855
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin) and urine
Assay Time: 90 Minutes

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Product Name

Rat Lipocalin-2/NGAL PicoKine® Quick ELISA Kit

See all Lipocalin-2/NGAL products

SKU/Catalog Number

FEK0855

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat Lipocalin-2/NGAL PicoKine™ Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Rat Lcn2 in cell culture supernatants, serum, plasma (heparin) and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Rat Lipocalin-2/NGAL PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0855)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q21-N198

Sensitivity

<10 pg/ml

Assay Range

78 pg/ml - 5,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of FEK0855 for more details

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

FEK0855 is reactive to LCN2 in Rat samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin) and urine

Application Guarantee

FEK0855 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Lipocalin-2/NGAL

Lipocalin-2 (LCN2), also known as NGAL, is a protein associated with neutrophil gelatinase.1 The LCN2 gene is located at 9q34 and contains 7 exons.2 The 25-kD LCN2 protein is believed to bind small lipophilic substances such as bacteria-derived lipopolysaccharide (LPS) and formylpeptides and may function as a modulator of inflammation. NGAL tightly binds bacterial catecholate-type ferric siderophores through a cyclically permuted, hybrid electrostatic/cation-pi interaction and is a potent bacteriostatic agent in iron-limiting conditions.3 The primary LCN2 transcript is 3,696 nucleotides long, and the processed transcript is 809 nucleotides long.4 LCN2 expression in adult bone marrow, uterus, prostate, salivary gland, stomach, appendix, colon, trachea, and lung, and in fetal spleen and lung. The standard product used in this kit is recombinant rat NGAL, consisting of 177 amino acids with the molecular mass of 21kDa.

Kit Components

Catalog numberDescription QuantityVolume
FEK0855-CAPAnti-tag Pre-coated 96-well Strip Microplate 112 strips of 8 wells
EK0855-STRat Lipocalin-2/NGAL Standard 2 10 ng/tube
FEK0855-ARat LCN2 Antibody A 1 3ml
FEK0855-BRat LCN2 Antibody B 1 3ml
AR1106-1Sample Diluent 1 15ml
AR1106-7TBS-T Wash Buffer (25x) 1 12ml
AR1104Color Developing Reagent (TMB) 1 10ml
AR1105Stop Solution 1 10ml
PLA-SEAAdhesive Plate Sealers 2 Piece

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional)
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.
  • Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)078156312625125025005000
O.D.0.0200.0610.1280.2360.4120.8351.3902.107

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Myeloblastin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)11776232641337823365
Standard deviation6.0849.53172.997.5860.21185.08
CV (%)5.2%6.5%5.3%5.7%7.7%5.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Lipocalin-2/NGAL in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11171221051131147.206.3%
Sample 276276368573073527.203.7%
Sample 332642569296332883021199.396.6%
*number of samples for each test n=16.

Gene/Protein Information For LCN2 (Source: Uniprot.Org, NCBI)

Gene Name

LCN2

Full Name

Neutrophil gelatinase-associated lipocalin

Weight

22.588kDa

Superfamily

calycin superfamily

Alternative Names

24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; HNL; LCN2; lipocalin 2 (oncogene 24p3); lipocalin 2; Lipocalin2; Lipocalin-2; migration-stimulating factor inhibitor; MSFI; neutrophil gelatinase-associated lipocalin; NGAL; NGALlipocalin-2; Oncogene 24p3; p25; Siderocalin; Uterocalin LCN2 24p3, MSFI, NGAL, p25 lipocalin 2 neutrophil gelatinase-associated lipocalin|25 kDa alpha-2-microglobulin-related subunit of MMP-9|migration-stimulating factor inhibitor|oncogene 24p3|siderocalin LCN2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on LCN2, check out the LCN2 Infographic

LCN2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for LCN2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

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