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Product Info Summary
| SKU: | EK0847 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants and cell lysates. |
Product info
Product Name
Rat TrkA ELISA Kit PicoKine®
SKU/Catalog Number
EK0847
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat TrkA ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ntrk1 in cell culture supernatants and cell lysates. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat TrkA ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0847)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A33-P418
Sensitivity
<10 pg/ml
Assay Range
78 pg/ml - 5,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0847 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0847 is reactive to NTRK1 in Rat samples
Validated Sample Types
cell culture supernatants and cell lysates.
Application Guarantee
EK0847 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TrkA
Tropomyosin receptor kinase A (TrkA) are efficacious in attenuating skeletal pain.1 TrkA mutants were able to activate signaling cascades and were even more efficient in promoting neurite outgrowth than the wild-type receptor.2 TrkA is part of a sub-family of protein kinases which includes TrkB and TrkC. Also, there are other neurotrophic factors structurally related to NGF: BDNF (for Brain-Derived Neurotrophic Factor), NT-3 (for Neurotrophin-3) and NT-4 (for Neurotrophin-4). While TrkA mediates the effects of NGF, TrkB is bound and activated by BDNF, NT-4, and NT-3. Further, TrkC binds and is activated by NT-3.3 TrkA receptor was found in keratoconus-affected corneas, along with an increased level of repressor isoform of Sp3 transcription factor.4 The standard product used in this kit is extracellular part (A33-P418) of recombinant rat TRKA, consisting of 386 amino acids with the molecular weight of 69KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0847-CAP | Anti-Rat NTRK1 Pre-coated 96-well strip microplate | 1 |
| EK0847-ST | Rat NTRK1 Standard | 2 vials, 10 ng/tube |
| EK0847-DA | Rat NTRK1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
| O.D. | 0.006 | 0.074 | 0.146 | 0.252 | 0.509 | 0.948 | 1.353 | 1.903 |
Data Example Images
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Rat TrkA PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat TrkA ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 115 | 863 | 2241 | 117 | 796 | 2366 |
| Standard deviation | 4.14 | 27.62 | 87.4 | 4.8 | 35.82 | 87.54 |
| CV(%) | 3.6% | 3.2 | 3.9% | 4.1% | 4.5% | 3.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TrkA in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 115 | 123 | 127 | 129 | 124 | 2.72 | 2.2% |
| Sample 2 | 863 | 769 | 752 | 806 | 798 | 16.75 | 2.1% |
| Sample 3 | 2241 | 2249 | 2369 | 2477 | 2334 | 72.35 | 3.1% |
Protein Target Info & Infographic
Gene/Protein Information For NTRK1 (Source: Uniprot.Org, NCBI)
Gene Name
NTRK1
Full Name
High affinity nerve growth factor receptor
Weight
87.497kDa
Superfamily
protein kinase superfamily
Alternative Names
DKFZp781I14186; EC 2.7.10; EC 2.7.10.1; MTChigh affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; neurotrophic tyrosine kinase, receptor, type 1; NTRK1; NTRK-1; p140-TrkA; TRK1-transforming tyrosine kinase protein; TrkA; Trk-A; TRKAOncogene TRK; TRKTRK1; tyrosine kinase receptor A NTRK1 MTC, TRK, TRK1, TRKA, Trk-A, p140-TrkA neurotrophic receptor tyrosine kinase 1 high affinity nerve growth factor receptor|Oncogene TRK|TRK1-transforming tyrosine kinase protein|gp140trk|neurotrophic tyrosine kinase, receptor, type 1|tropomyosin-related kinase A|tyrosine kinase receptor A
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on NTRK1, check out the NTRK1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for NTRK1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat TrkA ELISA Kit PicoKine® (EK0847)
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No publications found for EK0847
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Customer Reviews
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Customer Q&As
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12 Customer Q&As for Rat TrkA ELISA Kit PicoKine®
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-03-14
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-03-14
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring TrkA using the EK0847 Rat TrkA Picokine® ELISA Kit?
K. Phillips
Verified customer
Asked: 2020-02-09
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2020-02-09
Question
Q: Can TrkA ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
T. Wright
Verified customer
Asked: 2019-12-09
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-12-09
Question
Q: how do I analyze ELISA data? I have obtained TrkA level in cell lysates.
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-11-14
Question
Was a virus used to incorporate external gene for EK0847?
Verified customer
Asked: 2019-09-05
Answer
For the Rat TrkA ELISA Kit PicoKine™ (EK0847), no virus was used to incorporate external gene.
Boster Scientific Support
Answered: 2019-09-06
Question
What is the active ingredient of the stop solution and its concentration for EK0847?
Verified customer
Asked: 2019-09-03
Answer
For the Rat TrkA ELISA Kit PicoKine™ (EK0847), the active ingredient of the stop solution and its concentration is 2% H2SO4.
Boster Scientific Support
Answered: 2019-09-06
Question
What is the expression host of the standard for EK0847?
Verified customer
Asked: 2019-09-03
Answer
For the Rat TrkA ELISA Kit PicoKine™ (EK0847), the expression host of the standard Rat.
Boster Scientific Support
Answered: 2019-09-03
Question
What is the expression system of Lyophilized recombinant EK0847 standard? Do other components contain any recombinant proteins?
Verified customer
Asked: 2019-08-27
Answer
The expression system of Lyophilized recombinant rat TRKA standard is NSO. None of other components contain any recombinant proteins.
Boster Scientific Support
Answered: 2019-08-29
Question
What immunized animal and purification way of antibodies in EK0847?
Verified customer
Asked: 2019-08-27
Answer
For the Bovine Endothelin ELISA Kit PicoKine™ (EK0945-BV), Capture antibody: Goat polyclonal Affinity purified Detection antibody: Goat polyclonal Affinity purified
Boster Scientific Support
Answered: 2019-08-28
Question
Does EK0847 contain native human materials, is it purified or not?
Verified customer
Asked: 2019-08-27
Answer
The Rat TrkA ELISA Kit PicoKine™ (EK0847) doesn't contain any native human materials.
Boster Scientific Support
Answered: 2019-08-28
Question
Does EK0847 contain 2-tert-Butylphenol (CAS number: 88-18-6)?
Verified Customer
Verified customer
Asked: 2016-09-21
Answer
No, EK0847 does not contain 2-tert-Butylphenol (CAS number: 88-18-6).
Boster Scientific Support
Answered: 2016-09-21
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2015-04-26
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2015-04-26
