Overview
| Product Name |
Rat TrkA ELISA Kit PicoKine™
See more TrkA products |
| Catalog# |
EK0847 |
| Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Description |
Rat TrkA ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ntrk1 in cell culture supernatants and cell lysates.. Sensitivity: 10pg/ml. |
| Cite This Product |
Rat TrkA ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0847)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Rat |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants and cell lysates. |
| Sensitivity |
<10 pg/ml |
| Assay Range |
31.2 pg/ml - 2,000 pg/ml |
| Immunogen/Standard |
Expression system for standard: NS0; Immunogen sequence: A33-P418 |
| Antibody Clonalities |
Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For Ntrk1 (Source: Uniprot.org, NCBI)
| Uniprot Id | P35739 |
|---|
| NCBI Gene Id | 59109 |
|---|
| Species Of This Entry | Rat |
|---|
| Gene Name | Ntrk1 |
|---|
| Protein Name | High affinity nerve growth factor receptor |
|---|
| Superfamily | protein kinase superfamily |
|---|
| Alternative Names | TrkA|DKFZp781I14186; EC 2.7.10; EC 2.7.10.1; MTChigh affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; neurotrophic tyrosine kinase, receptor, type 1; NTRK1; NTRK-1; p140-TrkA; TRK1-transforming tyrosine kinase protein; TrkA; Trk-A; TRKAOncogene TRK; TRKTRK1; tyrosine kinase receptor A |
|---|
| Molecular Weight | 87868 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Ntrk1, check out the Ntrk1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Ntrk1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the Ntrk1 infographic
Kit Components And Assay QC Data Details Of Rat TrkA ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0847-CAP |
Anti-Rat Ntrk1 Pre-coated 96-well strip microplate | 1 |
| EK0847-ST | Rat Ntrk1 Standard | 2 vials, 10 ng/tube |
| EK0847-DA | Rat Ntrk1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0847 Rat TrkA ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.006 | 0.146 | 0.252 | 0.509 | 0.948 | 1.353 | 1.903 | 2.378 |
We measured random samples of Rat TrkA ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 51 | 251 | 890 | 52 | 254 | 917 |
| Standard deviation | 2.14 | 10.79 | 36.49 | 3.43 | 17.27 | 65.11 |
| CV (%) | 4.2% | 4.3% | 4.1% | 6.6% | 6.8% | 7.1% |
We measured the performance consistency of Rat TrkA ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 51 | 52 | 58 | 53 | 53 | 2.69 | 5.0% |
| Sample 2 | 251 | 252 | 244 | 229 | 244 | 9.19 | 3.7% |
| Sample 3 | 890 | 904 | 955 | 901 | 912 | 25.08 | 2.7% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 0
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0847 Rat TrkA ELISA Kit PicoKine™
12 Related Questions
Question
Does EK0847 contain 2-tert-Butylphenol (CAS number: 88-18-6)?
Verified Customer
Asked: 2016-09-21
Answer
No, EK0847 does not contain 2-tert-Butylphenol (CAS number: 88-18-6).
Boster Scientific Support
Answered: 2016-09-21
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Asked: 2020-03-14
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-03-14
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring TrkA using the EK0847 Rat TrkA Picokine® ELISA Kit?
K. Phillips
Asked: 2020-02-09
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2020-02-09
Question
Q: Can TrkA ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
T. Wright
Asked: 2019-12-09
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-12-09
Question
Q: how do I analyze ELISA data? I have obtained TrkA level in cell lysates.
Verified Customer
Asked: 2019-11-14
Boster Scientific Support
Answered: 2019-11-14
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Asked: 2015-04-26
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2015-04-26
Question
Was a virus used to incorporate external gene for EK0847?
Answer
For the Rat TrkA ELISA Kit PicoKine™ (EK0847), no virus was used to incorporate external gene.
Boster Scientific Support
Answered: 2019-09-06
Question
What is the active ingredient of the stop solution and its concentration for EK0847?
Answer
For the Rat TrkA ELISA Kit PicoKine™ (EK0847), the active ingredient of the stop solution and its concentration
is 2% H2SO4.
Boster Scientific Support
Answered: 2019-09-06
Question
What is the expression host of the standard for EK0847?
Answer
For the Rat TrkA ELISA Kit PicoKine™ (EK0847), the expression host of the standard Rat.
Boster Scientific Support
Answered: 2019-09-03
Question
What is the expression system of Lyophilized recombinant EK0847 standard? Do other components contain any recombinant proteins?
Answer
The expression system of Lyophilized recombinant rat TRKA standard is NSO. None of other components contain any recombinant proteins.
Boster Scientific Support
Answered: 2019-08-29
Question
What immunized animal and purification way of antibodies in EK0847?
Answer
For the Bovine Endothelin ELISA Kit PicoKine™ (EK0945-BV),
Capture antibody: Goat polyclonal Affinity purified
Detection antibody: Goat polyclonal Affinity purified
Boster Scientific Support
Answered: 2019-08-28
Question
Does EK0847 contain native human materials, is it purified or not?
Answer
The Rat TrkA ELISA Kit PicoKine™ (EK0847) doesn't contain any native human materials.
Boster Scientific Support
Answered: 2019-08-28
