Overview
| Product Name |
Rat TrkA ELISA Kit PicoKine™
See all TrkA primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
EK0847 |
| Storage & Handling |
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description |
Detect Rat P Trk/NTRK1 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates and cell lysates. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. P Trk/NTRK1 tissue specificity: Isoform Trka-II is primarily expressed in neuronal cells; isoform Trka-I is found in non-neuronal tissues. |
| Cite This Product |
Rat TrkA ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0847)
|
Product Specs
| Reactivity/Species |
Rat |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernates and cell lysates. |
| Sensitivity |
<10pg/ml |
| Assay Range |
31.2pg/ml-2000pg/ml |
| Immunogen/Standard |
Expression system for standard: NSO; Immunogen sequence: A33-P418 |
| Antibody Clonalities |
Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96wells/kit, with removable strips. |
Gene/Protein Basic Information For Ntrk1 (Source: Uniprot.org, NCBI)
| Uniprot Id | P35739 |
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| NCBI Gene Id | 59109 |
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| Species Of This Entry | Rat |
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| Gene Name | Ntrk1 |
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| Protein Name | High affinity nerve growth factor receptor |
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| Superfamily | protein kinase superfamily |
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| Alternative Names | TrkA|DKFZp781I14186; EC 2.7.10; EC 2.7.10.1; MTChigh affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; neurotrophic tyrosine kinase, receptor, type 1; NTRK1; NTRK-1; p140-TrkA; TRK1-transforming tyrosine kinase protein; TrkA; Trk-A; TRKAOncogene TRK; TRKTRK1; tyrosine kinase receptor A |
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| Molecular Weight | 87868 |
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| Post Tranlational Modifications | Phosphorylation; Methylation; Reduction; Glycosylation; Acetylation |
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| Gene Location | 2q34, on Chromasome 2, gene sequence: |
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*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Ntrk1 (Source: Uniprot.org, NCBI)
| Protein Function | Receptor tyrosine kinase involved in the development and the maturation of the central and peripheral nervous systems through regulation of proliferation, differentiation and survival of sympathetic and nervous neurons. High affinity receptor for NGF which is its primary ligand (PubMed:1312719, PubMed:1850821). Can also bind and be activated by NTF3/neurotrophin-3. However, NTF3 only supports axonal extension through NTRK1 but has no effect on neuron survival. Upon dimeric NGF ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades driving cell survival and differentiation. Through SHC1 and FRS2 activates a GRB2-Ras-MAPK cascade that regulates cell differentiation and survival. Through PLCG1 controls NF-Kappa-B activation and the transcription of genes involved in cell survival. Through SHC1 and SH2B1 controls a Ras-PI3 kinase-AKT1 signaling cascade that is also regulating survival. In absence of ligand and activation, may promote cell death, making the survival of neurons dependent on trophic factors. |
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| Tissue Specificity | Isoform Trka-II is primarily expressed in neuronal cells; isoform Trka-I is found in non-neuronal tissues. |
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| Related Diseases | Neoplasms; Pain; Thyroid Neoplasm; Malignant Neoplasms; Carcinoma; Anhidrosis; Hsan Type Iv; Hereditary Sensory And Autonomic Neuropathies; Malignant Neoplasm Of Thyroid; Carcinoma, Papillary; Papillary Thyroid Carcinoma; Neuroblastoma; Hypohidrosis; Nervousness; Hereditary Diseases; Congenital Pain Insensitivity; Cell Transformation, Neoplastic |
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| Related Pathways | Pathogenesis; Localization; Innervation; Cell Proliferation; Dna Methylation; Methylation; Interphase; Wound Healing; Oncogenesis; Dedifferentiation; Neural Crest Cell Differentiation; Cell Migration; Gene Silencing; Eating Behavior; Tube Morphogenesis; Reflex; Myelination; Inflammatory Response; Reverse Transcription; Mrna Splicing |
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| Background | Tropomyosin receptor kinase A(TrkA) are efficacious in attenuating skeletal pain.1 TrkA mutants were able to activate signaling cascades and were even more efficient in promoting neurite outgrowth than the wild-type receptor.2 TrkA is part of a sub-family of protein kinases which includes TrkB and TrkC. Also, there are other neurotrophic factors structurally related to NGF: BDNF(for Brain-Derived Neurotrophic Factor), NT-3(for Neurotrophin-3) and NT-4(for Neurotrophin-4). While TrkA mediates the effects of NGF, TrkB is bound and activated by BDNF, NT-4, and NT-3. Further, TrkC binds and is activated by NT-3.3 TrkA receptor was found in keratoconus-affected corneas, along with an increased level of repressor isoform of Sp3 transcription factor.4 The standard product used in this kit is extracellular part(A33-P418) of recombinant rat TRKA, consisting of 386 amino acids with the molecular weight of 69KDa. |
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| Scientific References | PMID: 1312719 by Meakin S.O., et al. The rat trk protooncogene product exhibits properties characteristic of the slow nerve growth factor receptor.
PMID: 8325889 by Barker P.A., et al. Tissue-specific alternative splicing generates two isoforms of the trkA receptor. |
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Kit Components And Assay QC Data Details Of Rat TrkA ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0847-CAP | 96-well plate precoated with anti-Rat TrkA antibody | 1 |
| EK0847-ST | lyophilized recombinant Rat TrkA standard | 10ng/tube |
| EK0847-DA | biotinylated anti-Rat TrkA antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0847 Rat TrkA ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.006 | 0.146 | 0.252 | 0.509 | 0.948 | 1.353 | 1.903 | 2.378 |
We measured random samples of Rat TrkA ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 51 | 251 | 890 | 52 | 254 | 917 |
| Standard deviation | 2.14 | 10.79 | 36.49 | 3.43 | 17.27 | 65.11 |
| CV(%) | 4.2% | 4.3% | 4.1% | 6.6% | 6.8% | 7.1% |
We measured the performance consistency of Rat TrkA ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 51 | 52 | 58 | 53 | 53 | 2.69 | 5.0% |
| Sample 2 | 251 | 252 | 244 | 229 | 244 | 9.19 | 3.7% |
| Sample 3 | 890 | 904 | 955 | 901 | 912 | 25.08 | 2.7% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all TrkA primary antibodies,
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