Rat TrkA ELISA Kit PicoKine®

TrkA ELISA kit for Rat

Rat TrkA ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ntrk1 in cell culture supernatants and cell lysates. Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0847
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants and cell lysates.

Product Name

Rat TrkA ELISA Kit PicoKine®

View all TrkA ELISA kits

SKU/Catalog Number

EK0847

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat TrkA ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ntrk1 in cell culture supernatants and cell lysates. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Rat TrkA ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0847)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A33-P418

Sensitivity

<10 pg/ml

Assay Range

78 pg/ml - 5,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0847 is reactive to Ntrk1 in Rat samples

Validated Sample Types

cell culture supernatants and cell lysates.

Application Guarantee

EK0847 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of TrkA

Tropomyosin receptor kinase A (TrkA) are efficacious in attenuating skeletal pain.1 TrkA mutants were able to activate signaling cascades and were even more efficient in promoting neurite outgrowth than the wild-type receptor.2 TrkA is part of a sub-family of protein kinases which includes TrkB and TrkC. Also, there are other neurotrophic factors structurally related to NGF: BDNF (for Brain-Derived Neurotrophic Factor), NT-3 (for Neurotrophin-3) and NT-4 (for Neurotrophin-4). While TrkA mediates the effects of NGF, TrkB is bound and activated by BDNF, NT-4, and NT-3. Further, TrkC binds and is activated by NT-3.3 TrkA receptor was found in keratoconus-affected corneas, along with an increased level of repressor isoform of Sp3 transcription factor.4 The standard product used in this kit is extracellular part (A33-P418) of recombinant rat TRKA, consisting of 386 amino acids with the molecular weight of 69KDa.

Kit Components

Catalog Number Description Quantity
EK0847-CAP Anti-Rat Ntrk1 Pre-coated 96-well strip microplate 1
EK0847-ST Rat Ntrk1 Standard 2 vials, 10 ng/tube
EK0847-DA Rat Ntrk1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)078156312625125025005000
O.D.0.0060.0740.1460.2520.5090.9481.3531.903

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TrkA, Dilution ratio of 1:1, concentration in serum is around 1ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat TrkA ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)11586322411177962366
Standard deviation4.1427.6287.44.835.8287.54
CV(%)3.6%3.23.9%4.1%4.5%3.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TrkA in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11151231271291242.722.2%
Sample 286376975280679816.752.1%
Sample 32241224923692477233472.353.1%
*number of samples for each test n=16.

Gene/Protein Information For Ntrk1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P35739

Gene ID

59109

Gene Name

Ntrk1

Full Name

High affinity nerve growth factor receptor

Weight

87.868kDa

Superfamily

protein kinase superfamily

Alternative Names

DKFZp781I14186; EC 2.7.10; EC 2.7.10.1; MTChigh affinity nerve growth factor receptor; Neurotrophic tyrosine kinase receptor type 1; neurotrophic tyrosine kinase, receptor, type 1; NTRK1; NTRK-1; p140-TrkA; TRK1-transforming tyrosine kinase protein; TrkA; Trk-A; TRKAOncogene TRK; TRKTRK1; tyrosine kinase receptor A Ntrk1|Trk|neurotrophic receptor tyrosine kinase 1|high affinity nerve growth factor receptor|TrkA neurotrophin receptor|neurotrophic tyrosine kinase, receptor, type 1|p140-TrkA|slow nerve growth factor receptor|trk-A|trkA proto-oncogene receptor|tropomyosin-related kinase A

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ntrk1, check out the Ntrk1 Infographic

Ntrk1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ntrk1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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12 Customer Q&As for Rat TrkA ELISA Kit PicoKine®

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-03-14

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-03-14

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring TrkA using the EK0847 Rat TrkA Picokine® ELISA Kit?

K. Phillips

Verified customer

Asked: 2020-02-09

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2020-02-09

Question

Q: Can TrkA ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

T. Wright

Verified customer

Asked: 2019-12-09

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-12-09

Question

Q: how do I analyze ELISA data? I have obtained TrkA level in cell lysates.

Verified Customer

Verified customer

Asked: 2019-11-14

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-11-14

Question

Was a virus used to incorporate external gene for EK0847?

Verified customer

Asked: 2019-09-05

Answer

For the Rat TrkA ELISA Kit PicoKine™ (EK0847), no virus was used to incorporate external gene.

Boster Scientific Support

Answered: 2019-09-06

Question

What is the active ingredient of the stop solution and its concentration for EK0847?

Verified customer

Asked: 2019-09-03

Answer

For the Rat TrkA ELISA Kit PicoKine™ (EK0847), the active ingredient of the stop solution and its concentration is 2% H2SO4.

Boster Scientific Support

Answered: 2019-09-06

Question

What is the expression host of the standard for EK0847?

Verified customer

Asked: 2019-09-03

Answer

For the Rat TrkA ELISA Kit PicoKine™ (EK0847), the expression host of the standard Rat.

Boster Scientific Support

Answered: 2019-09-03

Question

What is the expression system of Lyophilized recombinant EK0847 standard? Do other components contain any recombinant proteins?

Verified customer

Asked: 2019-08-27

Answer

The expression system of Lyophilized recombinant rat TRKA standard is NSO. None of other components contain any recombinant proteins.

Boster Scientific Support

Answered: 2019-08-29

Question

What immunized animal and purification way of antibodies in EK0847?

Verified customer

Asked: 2019-08-27

Answer

For the Bovine Endothelin ELISA Kit PicoKine™ (EK0945-BV), Capture antibody: Goat polyclonal Affinity purified Detection antibody: Goat polyclonal Affinity purified

Boster Scientific Support

Answered: 2019-08-28

Question

Does EK0847 contain native human materials, is it purified or not?

Verified customer

Asked: 2019-08-27

Answer

The Rat TrkA ELISA Kit PicoKine™ (EK0847) doesn't contain any native human materials.

Boster Scientific Support

Answered: 2019-08-28

Question

Does EK0847 contain 2-tert-Butylphenol (CAS number: 88-18-6)?

Verified Customer

Verified customer

Asked: 2016-09-21

Answer

No, EK0847 does not contain 2-tert-Butylphenol (CAS number: 88-18-6).

Boster Scientific Support

Answered: 2016-09-21

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2015-04-26

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2015-04-26

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