Anti-14-3-3 Rabbit Monoclonal Antibody
|Reactivity||Human, Mouse, Rat|
|Product Name||Anti-14-3-3 Rabbit Monoclonal Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.|
|Description||Anti-14-3-3 Rabbit Monoclonal Antibodytested for Flow Cytometry, IHC, WB in Human, Mouse, Rat|
|Cite This Product||Anti-14-3-3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02431-1)|
|Contents/Buffer||Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.|
|Immunogen||A synthesized peptide derived from human 41701|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Flow Cytometry 1:50
Images And Assay Conditions
Western blot analysis of 14-3-3 expression in Hela lysate (M02431-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YWHAB monoclonal antibody (Catalog # M02431-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YWHAB
Protein Target Info (Source: Uniprot.org)
|Protein Name||14-3-3 protein beta/alpha|
|Alternative Names||14-3-3 protein beta/alpha;Protein 1054;Protein kinase C inhibitor protein 1;KCIP-1;14-3-3 protein beta/alpha, N-terminally processed;YWHAB;|
|Subcellular Localization||Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.|
|Molecular Weight||28082 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activatation of MAP kinases via AKAP13. .|
|Research Areas||Human, Mouse, Rat
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