Anti-14-3-3 Rabbit Monoclonal Antibody


SKU M02431-1
Size 100ug/vial
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number HHO-25
Host Rabbit
Applications WB

Overview

Product Name Anti-14-3-3 Rabbit Monoclonal Antibody
SKU/Catalog Number M02431-1
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100ug/vial
Description Anti-14-3-3 Rabbit Monoclonal Antibodytested for Flow Cytometry, IHC, WB in Human, Mouse, Rat
Cite This Product Anti-14-3-3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02431-1)
Host Rabbit
Contents/Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Immunogen A synthesized peptide derived from human 41701
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
WB 1:500-1:1000
IHC 1:50-1:200
Flow Cytometry 1:50

Images And Assay Conditions

Western blot analysis of 14-3-3 expression in Hela lysate (M02431-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YWHAB monoclonal antibody (Catalog # M02431-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YWHAB

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P31946
Gene Name YWHAB
Protein Name 14-3-3 protein beta/alpha
Alternative Names 14-3-3 protein beta/alpha;Protein 1054;Protein kinase C inhibitor protein 1;KCIP-1;14-3-3 protein beta/alpha, N-terminally processed;YWHAB;
Subcellular Localization Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Molecular Weight 28082 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activatation of MAP kinases via AKAP13. .
Research Areas Human, Mouse, Rat

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Monoclonal antibody for 14 3 3 BETA/YWHAB detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IHC, WB. Reactive species: Human, Mouse, Rat 14 3 3 BETA/YWHAB information: Molecular Weight: 28082 MW; Subcellular Localization: Cytoplasm . Melanosome . Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
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In stock
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M02431-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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