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Product Info Summary
| SKU: | A01072 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-ACTA2/Alpha Smooth Muscle Actin Monoclonal Antibody
SKU/Catalog Number
A01072
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ACTA2/Alpha Smooth Muscle Actin Monoclonal Antibody catalog # A01072. Tested in WB, IHC, IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACTA2/Alpha Smooth Muscle Actin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01072)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
4A4
Isotype
IgG
Immunogen
Synthesized peptide of human ACTA2.
Reactive Species
A01072 is reactive to ACTA2 in Human, Monkey, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
42.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01072 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
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Flow cytometric analysis of Hela cells using ACTA2 Monoclonal Antibody (green) and negative control (purple).
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Western Blot analysis using ACTA2 Monoclonal Antibody against HeLa (1), A431 (2), Jurkat (3), K562 (4), HEK293 (5), HepG2 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.
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Immunohistochemistry analysis of paraffin-embedded human duodenum tissues (left) and human esophagus tissues (right) with DAB staining using ACTA2 Monoclonal Antibody.
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Immunofluorescence analysis of HepG2 cells using ACTA2 Monoclonal Antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
Specific Publications For Anti-ACTA2/Alpha Smooth Muscle Actin Monoclonal Antibody (A01072)
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