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Product Info Summary
|Reactive Species:||Human, Monkey, Mouse, Rat|
|Application:||ELISA, Flow Cytometry, IF, IHC, WB|
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Anti-ACTA2/Alpha Smooth Muscle Actin Monoclonal Antibody
Boster Bio Anti-ACTA2/Alpha Smooth Muscle Actin Monoclonal Antibody catalog # A01072. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ACTA2/Alpha Smooth Muscle Actin Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01072)
Ascitic fluid containing 0.03% sodium azide.
Synthesized peptide of human ACTA2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
A01072 is reactive to ACTA2 in Human, Monkey, Mouse, Rat
A01072 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Validation Images & Assay Conditions
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Western Blot (WB) analysis using ACTA2 Monoclonal antibody against HeLa (1), A431 (2), Jurkat (3), K562 (4), HEK293 (5), HepG2 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.
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Immunohistochemistry (IHC) analysis of paraffin-embedded human duodenum tissues (left) and human esophagus tissues (right) with DAB staining using ACTA2 Monoclonal antibody.
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Flow cytometric (FCM) analysis of HeLa cells using ACTA2 Monoclonal antibody (green) and negative control (purple).
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Immunofluorescence (IF) analysis of HepG2 cells using ACTA2 Monoclonal antibody (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin. Blue: DRAQ5 fluorescent DNA dye.
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ELISA analysis of ACTA2 antibody.
Gene/Protein Information For ACTA2 (Source: Uniprot.Org, NCBI)
Actin, aortic smooth muscle
1a4 sma; 1a4 smooth muscle actin; AAT6; ACTA2; Actin alpha 2; actin, alpha 2, smooth muscle, aorta; actin, aortic smooth muscle; ACTSA; ACTVS; alpha 2 actin; alpha sma 1a4; alpha SMA; alpha smooth muscle actin; alpha-actin-2; alpha-SMA; alphaSmooth Muscle Actin; alpha-Smooth Muscle Actin; a-sma; Cell growth-inhibiting gene 46 protein; growth-inhibiting gene 46; MYMY5; sma 1a4; SMA; smooth muscle actin ACTA2 ACTSA actin alpha 2, smooth muscle actin, aortic smooth muscle|actin, alpha 2, smooth muscle, aorta|alpha-cardiac actin|cell growth-inhibiting gene 46 protein*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ACTA2, check out the ACTA2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ACTA2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
A01072 has been cited in 70 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Fang L,Wang KK,Zhang PF,Li T,Xiao ZL,Yang M,Yu ZX.Nucleolin promotes Ang II-induced phenotypic transformation of vascular smooth muscle cells by regulating EGF and PDGF-BB.J Cell Mol Med.2020 Jan;24(2) :1917-1933.doi:10.1111/jcmm.14888.Epub 2020 Jan 1.PMI
A01072 usage in article: APP:WB, SAMPLE:VSMCS, DILUTION:NA
Shen J,Wei W,Wang X,Yang J,Lu L,Lv X,Xue X.Proliferation of Vascular Smooth Muscle Cells under ox-LDL Is Regulated by Alismatis rhizoma Decoction via InhibitingERK1/2 and miR-17∼92a Cluster Activation.Evid Based Complement Alternat Med.2020 Aug 22;2020:72
A01072 usage in article: APP:IHC, SAMPLE:VSMCS, DILUTION:1:1000
Xu T,Pan L,Li L,Hu S,Zhou H,Yang C,Yang J,Li H,Liu Y,Meng X,Li J.MicroRNA-708 modulates Hepatic Stellate Cells activation and enhances extracellular matrix accumulation via direct targeting TMEM88. J Cell Mol Med. 2020 Jul;24(13):7127-7140.doi: 10.1111/jc
A01072 usage in article: APP:WB, SAMPLE:LX-2 CELLS, DILUTION:1:1000
Wang R, Wu G, Dai T, Lang Y, Chi Z, Yang S, Dong D. Naringin attenuates renal interstitial fibrosis by regulating the TGF-β/Smad signaling pathway and inflammation. Exp Ther Med. 2021 Jan;21(1):66. doi: 10.3892/etm.2020.9498. Epub 2020 Nov 23. PMID: 33365
A01072 usage in article: APP:IF, SAMPLE:KIDNEY TISSUE, DILUTION:1:70
Liao J, Wu Q, Qian C, Zhao N, Zhao Z, Lu K, Zhang S, Dong Q, Chen L, Li Q, Du Y. TRPV4 blockade suppresses atrial fibrillation in sterile pericarditis rats. JCI Insight. 2020 Oct 29:137528. doi: 10.1172/jci.insight.137528. Epub ahead of print. PMID: 33119
A01072 usage in article: APP:IHC, SAMPLA:ATRIA TISSUE, DILUTION:1:50
Yang J, Li J, Tan R, He X, Lin X, Zhong X, Fan J, Wang L. Protocatechualdehyde attenuates obstructive nephropathy through inhibiting lncRNA9884 induced inflammation. Phytother Res. 2020 Oct 28. doi:10.1002/ptr.6 919. Epub ahead of print. PMID: 33118280.
A01072 usage in article: APP:WB, SAMPLE:MOUSE KIDNEY TISSUES, DILUTION:1:1000
Extracorporeal shock wave therapy with low-energy flux density inhibits hypertrophic scar formation in an animal model
The Role and Mechanism of ?-Klotho in the Calcification of Rat Aortic Vascular Smooth Muscle Cells
The rhythmic expression of clock genes attenuated in human plaque-derived vascular smooth muscle cells
Treatment of diabetes mellitus-induced erectile dysfunction using endothelial progenitor cells genetically modified with human telomerase reverse transcriptase
Inhibitory effects of dimethyl ?-ketoglutarate in hepatic stellate cell activation
The protective role of vitamin D3 in a murine model of asthma via the suppression of TGF-?/Smad signaling and activation of the Nrf2/HO-1 pathway
Functional and Proteomic Investigations Reveal Major Royal Jelly Protein 1 Associated with Anti-hypertension Activity in Mouse Vascular Smooth MuscleCells
Signal Transducer and Activator of Transcription 3/MicroRNA-21 Feedback Loop Contributes to Atrial Fibrillation by Promoting Atrial Fibrosis in a Rat Sterile Pericarditis Model
Imperatorin derivative OW1 inhibits the upregulation of TGF-? and MMP-2 in renovascular hypertension-induced cardiac remodeling
Macrophage migration inhibitory factor promotes cardiac stem cell proliferation and endothelial differentiation through the activation of the PI3K/Akt/mTOR and AMPK pathways
The Endothelial Prolyl-4-Hydroxylase Domain 2/Hypoxia-Inducible Factor 2 Axis Regulates Pulmonary Artery Pressure in Mice
Blockade of Orai1 Store-Operated Calcium Entry Protects against Renal Fibrosis
Effect of Huai Qi Huang on Epithelial-Mesenchymal Transition of Renal Tubular Epithelial Cells through miR-200a
Digoxin reduces atherosclerosis in apolipoprotein E-deficient mice
Polarization-sensitive optical projection tomography for muscle fiber imaging
Wnt/?-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells
Identification of cardiac-related circulating microRNA profile in human chronic heart failure
Effect of P2X4R on airway inflammation and airway remodeling in allergic airway challenge in mice
Mesenchymal stem cell-laden anti-inflammatory hydrogel enhances diabetic wound healing.
RAC1 overexpression promotes the proliferation, migration and epithelial-mesenchymal transition of lens epithelial cells
Plumbagin Ameliorates CCl4-Induced Hepatic Fibrosis in Rats via the Epidermal Growth Factor Receptor Signaling Pathway
Involvement of epithelial-to-mesenchymal transition and associated transforming growth factor-?/Smad signaling in paraquat-induced pulmonary fibrosis
Modulation of fatty acid metabolism is involved in the alleviation of isoproterenol-induced rat heart failure by fenofibrate.
Glycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in rats
Tissue Transglutaminase-Regulated Transformed Growth Factor-?1 in the Parasite Links Schistosoma japonicum Infection with Liver Fibrosis
Overexpression of monocarboxylate anion transporter 1 and 4 in T24-induced cancer-associated fibroblasts regulates the progression of bladder cancer cells in a 3D microfluidic device
Dynamic Contrast-Enhanced Magnetic Resonance Imaging with Gd-EOB-DTPA for the Evaluation of Liver Fibrosis Induced by Carbon Tetrachloride in Rats
Mig-6Gene Knockout Induces Neointimal Hyperplasia in the Vascular Smooth Muscle Cell
Rho-Associated Protein Kinases Play an Important Role in the Differentiation of Rat Adipose-Derived Stromal Cells into Cardiomyocytes In Vitro
Clinicopathological and prognostic significance of epithelial mesenchymal transition-related protein expression in intrahepatic cholangiocarcinoma
Suppression of CB1 Cannabinoid Receptor by Lentivirus Mediated Small Interfering RNA Ameliorates Hepatic Fibrosis in Rats
Effects of PPARg agonist pioglitazone on rat hepatic fibrosis
Delta-like 1 Serves as a New Target and Contributor to Liver Fibrosis Down-regulated by Mesenchymal Stem Cell Transplantation
Anti-heat shock protein 70 autoantibody epitope changes and BD091 promotes atherosclerosis in rats
Regulation of smooth muscle contractility by the epithelium in rat vas deferens: role of ATP-induced release of PGE2
RNA interference targeting the platelet-derived growth factor receptor ? subunit ameliorates experimental hepatic fibrosis in rats
TGF-?1 promotes scar fibroblasts proliferation and transdifferentiation via up-regulating MicroRNA-21
Reduced Expression of the Extracellular Calcium-Sensing Receptor (CaSR) Is Associated with Activation of the Renin-Angiotensin System (RAS) to Promote Vascular Remodeling in the Pathogenesis of Essential Hypertension
Preventive Effects of Rhodiola rosea L on Bleomycin-Induced Pulmonary Fibrosis in Rats
BMSCs Interactions with Adventitial Fibroblasts Display Smooth Muscle Cell Lineage Potential in Differentiation and Migration That Contributes to Neointimal Formation
Salidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-?B and TGF-?1/Smad-2/-3 pathways
Silica promotes the transdifferentiation of rat circulating fibrocytes in vitro
Pan Y, Liu L, Li S, Wang K, Ke R, Shi W, Wang J, Yan X, Zhang Q, Wang Q, Chai L, Xie X, Li M. Sci Rep. 2018 Feb 26;8(1):3624. doi: 10.1038/s41598-018-21812-0. Activation of AMPK inhibits TGF-β1-induced airway smooth muscle cells proliferation and...
Liu Z,, Xu S,, Huang X, Wang J, Gao S, Li H, Zhou C, Ye J, Chen S, Jin Zg, Liu P. Br J Pharmacol. 2015 Dec;172(23):5661-75. Doi: 10.1111/Bph.13068. Epub 2015 Feb 27. Cryptotanshinone, An Orally Bioactive Herbal Compound From Danshen, Attenuates At...
Li J, Ye Y, Zhang R, Zhang L, Hu X, Han D, Chen J, He X, Wang G, Yang X, Wang L. Sci Rep. 2015 May 19;5:9720. Doi: 10.1038/Srep09720. Robo1/2 Regulate Follicle Atresia Through Manipulating Granulosa Cell Apoptosis In Mice.
Yuan W, Yang D, Sun X, Liu W, Wang L, Li X, Man X, Fu Q. Int J Clin Exp Pathol. 2014 Aug 15;7(9):5772-81. Ecollection 2014. Effects Of Hydroxysafflor Yellow A On Proliferation And Collagen Synthesis Of Rat Vascular Adventitial Fibroblasts Induced ...
Yang L, Ni L, Duan Q, Wang X, Chen C, Chen S, Chaugai S, Zeldin Dc, Tang Jr, Wang Dw. Prostaglandins Other Lipid Mediat. 2015 Jan-Mar;116-117:64-75. Doi: 10.1016/J.Prostaglandins.2015.01.004. Epub 2015 Feb 14. Cyp Epoxygenase 2J2 Prevents Cardiac ...
Hu J, Zhou Z, Shi S, Zhu X, Wang X, Zhang W, Hu S, Qian H, Xu W. Oncol Lett. 2013 Jan;5(1):179-184. Epub 2012 Oct 30. Mesenchymal Stem-Like Cells Isolated From Human Esophageal Carcinoma And Adjacent Non-Cancerous Tissues.
Tang J, Huang H, Ji X, Zhu X, Li Y, She M, Yan S, Fung M, Li Z. Mediators Inflamm. 2014;2014:753483. Doi: 10.1155/2014/753483. Epub 2014 Jul 3. Involvement Of Il-13 And Tissue Transglutaminase In Liver Granuloma And Fibrosis After Schistosoma Japo...
Zhang H, Ren Y, Tang X, Wang K, Liu Y, Zhang L, Li X, Liu P, Zhao C, He J. Sci Rep. 2015 Mar 9;5:8888. Doi: 10.1038/Srep08888. Vascular Normalization Induced By Sinomenine Hydrochloride Results In Suppressed Mammary Tumor Growth And Metastasis.
Song Y, Zhan L, Yu M, Huang C, Meng X, Ma T, Zhang L, Li J. Plos One. 2014 Jul 11;9(7):E101179. Doi: 10.1371/Journal.Pone.0101179. Ecollection 2014. Trpv4 Channel Inhibits Tgf-??1-Induced Proliferation Of Hepatic Stellate Cells.
You H, Wei L, Sun Wl, Wang L, Yang Zl, Liu Y, Zheng K, Wang Y, Zhang Wj. Int J Mol Med. 2014 Jul;34(1):92-102. Doi: 10.3892/Ijmm.2014.1745. Epub 2014 Apr 16. The Green Tea Extract Epigallocatechin-3-Gallate Inhibits Irradiation-Induced Pulmonary F...
Song Y, Zhu L, Li M. Clinics (Sao Paulo). 2013 Oct;68(10):1350-7. Doi: 10.6061/Clinics/2013(10)10. Antifibrotic Effects Of Crocetin In Scleroderma Fibroblasts And In Bleomycin-Induced Sclerotic Mice.
Jiang B, Li D, Deng Y, Teng F, Chen J, Xue S, Kong X, Luo C, Shen X, Jiang H, Xu F, Yang W, Yin J, Wang Y, Chen H, Wu W, Liu X, Guo Da. Plos One. 2013;8(3):E59621. Doi: 10.1371/Journal.Pone.0059621. Epub 2013 Mar 22. Salvianolic Acid A, A Novel Ma...
Hu H, Xuan Y, Wang Y, Xue M, Suo F, Li X, Cheng W, Li X, Yin J, Liu J, Yan S. Plos One. 2014 Apr 22;9(4):E95106. Doi: 10.1371/Journal.Pone.0095106. Ecollection 2014. Targeted Ngf Sirna Delivery Attenuates Sympathetic Nerve Sprouting And Deteriorat...
Wang Lp, Dong Jz, Xiong Lj, Shi Kq, Zou Zl, Zhang Sn, Cao St, Lin Z, Chen Yp. Int J Clin Exp Pathol. 2014 Jun 15;7(7):3537-47. Ecollection 2014. Bmp-7 Attenuates Liver Fibrosis Via Regulation Of Epidermal Growth Factor Receptor.
Tang J, Jiang X, Zhou Y, Xia B, Dai Y. Exp Ther Med. 2015 Mar;9(3):737-743. Epub 2015 Jan 13. Increased Adenosine Levels Contribute To Ischemic Kidney Fibrosis In The Unilateral Ureteral Obstruction Model.
Liu Zh, Fan W, Chen Rc. Int J Clin Exp Pathol. 2015 Apr 1;8(4):3441-50. Ecollection 2015. 3,4-Dihydroxyphenylethanol Suppresses Irradiation-Induced Pulmonary Fibrosis In Adult Rats.
Qiu X,,, Fu K,, Zhao X,, Zhang Y, Yuan Y, Zhang S, Gu X, Guo H. J Transl Med. 2015 Jan 27;13:28. Doi: 10.1186/S12967-015-0388-1. Protective Effects Of Astaxanthin Against Ischemia/Reperfusion Induced Renal Injury In Mice.
Chen Jl, Shang Qh, Hu W, Liu C, Mao Wh, Liu Hq. J Geriatr Cardiol. 2012 Jun;9(2):185-91. Doi: 10.3724/Sp.J.1263.2012.04011. Role Of Tgf-??1/Smads Pathway In Carotid Artery Remodeling In Renovascular Hypertensive Rats And Prevention By Enalapril An...
Bai J, Liu H, Li D, Cui L, Wu X. Plos One. 2012;7(11):E49673. Doi: 10.1371/Journal.Pone.0049673. Epub 2012 Nov 20. Duodenum Clamping Trauma Induces Significant Postoperative Intraperitoneal Adhesions On A Rat Model.
Zhou L, Yang Y, Tian D, Wang Y. Oncol Rep. 2013 Mar;29(3):875-84. Doi: 10.3892/Or.2013.2227. Epub 2013 Jan 4. Oxidative Stress-Induced 1, N6-Ethenodeoxyadenosine Adduct Formation Contributes To Hepatocarcinogenesis.
Zhang R, Zhou Sj, Li Cj, Wang Xn, Tang Yz, Chen R, Lv L, Zhao Q, Xing Ql, Yu Dm, Yu P. Lipids Health Dis. 2013 Mar 26;12:42. Doi: 10.1186/1476-511X-12-42. C-Reactive Protein/Oxidised Low-Density Lipoprotein/??2-Glycoprotein I Complex Promotes Athe...
Shan Zy, Wu Ys, Li X, Shen Xh, Wang Zd, Liu Zh, Shen Jl, Lei L. Cell Reprogram. 2014 Feb;16(1):77-83. Doi: 10.1089/Cell.2013.0067. Epub 2014 Jan 4. Continuous Passages Accelerate The Reprogramming Of Mouse Induced Pluripotent Stem Cells.
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