Product Info Summary
SKU: | M00546 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ALDH2 Monoclonal Antibody
SKU/Catalog Number
M00546
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-ALDH2 Monoclonal Antibody catalog # M00546. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ALDH2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00546)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFOI-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human ALDH2 Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00546 is reactive to ALDH2 in Human, Mouse, Rat
Applications
M00546 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
52 kDa
Calculated molecular weight
56.381kDa
Background of ALDH2
Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Figure 1. Western blot analysis of CDH2 using anti-CDH2 antibody (M01577-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: rat heart tissue lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDH2 antigen affinity purified monoclonal antibody (Catalog # M01577-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDH2 at approximately 140 kDa. The expected band size for CDH2 is at 100 kDa.
Click image to see more details
Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
Protein Target Info & Infographic
Gene/Protein Information For ALDH2 (Source: Uniprot.org, NCBI)
Gene Name
ALDH2
Full Name
Aldehyde dehydrogenase, mitochondrial
Weight
56.381kDa
Superfamily
aldehyde dehydrogenase family
Alternative Names
acetaldehyde dehydrogenase 2; aldehyde dehydrogenase 2 family (mitochondrial); aldehyde dehydrogenase, mitochondrial; ALDH class 2; ALDH2; ALDH-E2; ALDHI; ALDM; EC 1.2.1; EC 1.2.1.3; liver mitochondrial ALDH; MGC1806; nucleus-encoded mitochondrial aldehyde dehydrogenase 2 ALDH2 ALDH-E2, ALDHI, ALDM aldehyde dehydrogenase 2 family member aldehyde dehydrogenase, mitochondrial|ALDH class 2|acetaldehyde dehydrogenase 2|aldehyde dehydrogenase 2 family (mitochondrial)|epididymis secretory sperm binding protein|liver mitochondrial ALDH|nucleus-encoded mitochondrial aldehyde dehydrogenase 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ALDH2, check out the ALDH2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ALDH2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ALDH2 Monoclonal Antibody (M00546)
Hello CJ!
M00546 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li Y,Chen F,Chen J,Chan S,He Y,Liu W,Zhang G.Disulfiram/Copper Induces Antitumor Activity against Both Nasopharyngeal Cancer Cells and Cancer-Associated Fibroblasts through ROS/MAPK and Ferroptosis Pathways.Cancers (Basel).2020 Jan 6;12(1):138.doi:10.3390
Species: Human,Mouse
M00546 usage in article: APP:WB, SAMPLE:NPC CELL LINES AND NP69 CELL, DILUTION:1:100
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