Anti-Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Monoclonal Antibody

Alkaline Phosphatase/ALPP antibody

Boster Bio Anti-Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Monoclonal Antibody (Catalog # M01718). Tested in WB, Flow Cytometry, IF, IHC applications. This antibody reacts with Human.

Product Info Summary

SKU: M01718
Size: 100ug/vial
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, IF, IHC, WB

Product Name

Anti-Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Monoclonal Antibody

View all Alkaline Phosphatase/ALPP Antibodies

SKU/Catalog Number

M01718

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Monoclonal Antibody (Catalog # M01718). Tested in WB, Flow Cytometry, IF, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01718)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: PL8-F6

Isotype

IgG2b, kappa

Immunogen

Purified human PLAP protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01718 is reactive to ALPP in Human

Applications

M01718 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

57.954kDa

Background of Alkaline Phosphatase/ALPP

Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Frozen & Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues.)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For ALPP (Source: Uniprot.org, NCBI)

Gene Name

ALPP

Full Name

Alkaline phosphatase, placental type

Weight

57.954kDa

Superfamily

alkaline phosphatase family

Alternative Names

Alkaline phosphatase Regan isozyme; Alkaline Phosphatase; alkaline phosphatase, placental (Regan isozyme); alkaline phosphatase, placental type; alkaline phosphatase, placental; alkaline phosphomonoesterase; ALP; ALPP; EC 3.1.3.1; FLJ61142; glycerophosphatase; PALP; Placental alkaline phosphatase 1; PLAP; PLAP-1; Regan isozyme; SEAP ALPP ALP, ALPI, IAP, PALP, PLAP, PLAP-1 alkaline phosphatase, placental alkaline phosphatase, placental type|Intestinal alkaline phosphatase|Intestinal-type alkaline phosphatase|alkaline phosphatase Regan isozyme|alkaline phosphomonoesterase|glycerophosphatase|placental alkaline phosphatase 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ALPP, check out the ALPP Infographic

ALPP infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ALPP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for M01718

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Monoclonal Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Monoclonal Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

3 Customer Q&As for Anti-Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Monoclonal Antibody

Question

We are currently using anti-Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Monoclonal antibody M01718 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?

K. Li

Verified customer

Asked: 2019-10-15

Answer

The anti-Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Monoclonal antibody (M01718) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-15

Question

My colleagues were well pleased with the WB result of your anti-Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Monoclonal antibody. However we have been able to see positive staining in cervix carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is ALPP supposed to be expressed?

D. Patel

Verified customer

Asked: 2016-09-16

Answer

According to literature, cervix carcinoma does express ALPP. Generally ALPP expresses in cell membrane. Regarding which tissues have ALPP expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix, and Placenta, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2016-09-16

Question

We have observed staining in human cervix placenta. Are there any suggestions? Is anti-Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Monoclonal antibody supposed to stain cervix placenta positively?

F. Taylor

Verified customer

Asked: 2014-10-16

Answer

From literature cervix placenta does express ALPP. From Uniprot.org, ALPP is expressed in placenta, cervix placenta, cervix carcinoma, among other tissues. Regarding which tissues have ALPP expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix, and Placenta, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2014-10-16

Size

Total: $468

SKU:M01718

Backordered.

Lead time for this item is typically 6-8 days

Get A Quote
Eddy test
In stock
Order Product
M01718
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$468.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.