Product Info Summary
SKU: | M01718 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Monoclonal Antibody
View all Alkaline Phosphatase/ALPP Antibodies
SKU/Catalog Number
M01718
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Monoclonal Antibody (Catalog # M01718). Tested in WB, Flow Cytometry, IF, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01718)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: PL8-F6
Isotype
IgG2b, kappa
Immunogen
Purified human PLAP protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M01718 is reactive to ALPP in Human
Applications
M01718 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
57.954kDa
Background of Alkaline Phosphatase/ALPP
Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Reconsitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot (1-2ug/ml)
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Frozen & Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT)(No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues.)Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human Placenta stained with Anti-PLAP Monoclonal Antibody (PL8-F6).
Click image to see more details
Western Blot analysis of JEG-3 cell lysate using Anti-PLAP Monoclonal Antibody (PL8-F6).
Protein Target Info & Infographic
Gene/Protein Information For ALPP (Source: Uniprot.org, NCBI)
Gene Name
ALPP
Full Name
Alkaline phosphatase, placental type
Weight
57.954kDa
Superfamily
alkaline phosphatase family
Alternative Names
Alkaline phosphatase Regan isozyme; Alkaline Phosphatase; alkaline phosphatase, placental (Regan isozyme); alkaline phosphatase, placental type; alkaline phosphatase, placental; alkaline phosphomonoesterase; ALP; ALPP; EC 3.1.3.1; FLJ61142; glycerophosphatase; PALP; Placental alkaline phosphatase 1; PLAP; PLAP-1; Regan isozyme; SEAP ALPP ALP, ALPI, IAP, PALP, PLAP, PLAP-1 alkaline phosphatase, placental alkaline phosphatase, placental type|Intestinal alkaline phosphatase|Intestinal-type alkaline phosphatase|alkaline phosphatase Regan isozyme|alkaline phosphomonoesterase|glycerophosphatase|placental alkaline phosphatase 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ALPP, check out the ALPP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ALPP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Monoclonal Antibody (M01718)
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No publications found for M01718
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Monoclonal Antibody
Question
We are currently using anti-Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Monoclonal antibody M01718 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
K. Li
Verified customer
Asked: 2019-10-15
Answer
The anti-Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Monoclonal antibody (M01718) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-15
Question
My colleagues were well pleased with the WB result of your anti-Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Monoclonal antibody. However we have been able to see positive staining in cervix carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is ALPP supposed to be expressed?
D. Patel
Verified customer
Asked: 2016-09-16
Answer
According to literature, cervix carcinoma does express ALPP. Generally ALPP expresses in cell membrane. Regarding which tissues have ALPP expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix, and Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-09-16
Question
We have observed staining in human cervix placenta. Are there any suggestions? Is anti-Alkaline Phosphatase (Placental) / PLAP (Germ Cell Tumor Marker) Monoclonal antibody supposed to stain cervix placenta positively?
F. Taylor
Verified customer
Asked: 2014-10-16
Answer
From literature cervix placenta does express ALPP. From Uniprot.org, ALPP is expressed in placenta, cervix placenta, cervix carcinoma, among other tissues. Regarding which tissues have ALPP expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648
Cervix, and Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2014-10-16