Product Info Summary
SKU: | A00522-3 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-alpha 1 Fetoprotein/AFP Antibody Picoband™
View all alpha-Fetoprotein/AFP Antibodies
SKU/Catalog Number
A00522-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-alpha 1 Fetoprotein/AFP Antibody Picoband™ catalog # A00522-3. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-alpha 1 Fetoprotein/AFP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00522-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human alpha 1 Fetoprotein/AFP recombinant protein (Position: R19-L258).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00522-3 is reactive to AFP in Human
Applications
A00522-3 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
69 kDa
Calculated molecular weight
68.678kDa
Background of alpha-Fetoprotein/AFP
AFP, also called Alpha-fetoprotein; alpha-fetoprotein, is a protein that in humans is encoded by the AFP gene. It is mapped to 4q13.3. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. In rodents AFP binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. Moreover, it has an important role as a diagnostic marker, especially in certain tumors and liver diseases of childhood. AFP is also used to test the potential usefulness of plasma alpha fetoprotein determination as a diagnostic marker in biliary atresia, hepatitis, and yolk sac derived tumours.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of alpha 1 Fetoprotein/AFP using anti-alpha 1 Fetoprotein/AFP antibody (A00522-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human HCCP tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-alpha 1 Fetoprotein/AFP antigen affinity purified polyclonal antibody (Catalog # A00522-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for alpha 1 Fetoprotein/AFP at approximately 69 kDa. The expected band size for alpha 1 Fetoprotein/AFP is at 69 kDa.
Protein Target Info & Infographic
Gene/Protein Information For AFP (Source: Uniprot.org, NCBI)
Gene Name
AFP
Full Name
Alpha-fetoprotein
Weight
68.678kDa
Superfamily
ALB/AFP/VDB family
Alternative Names
AFP; Alpha-1-fetoprotein; alpha-fetoglobulin; alpha-Fetoprotein; DSCAM2; FETA; HP; HPAFP AFP AFPD, FETA, HPAFP alpha fetoprotein alpha-fetoprotein|alpha-1-fetoprotein|alpha-fetoglobulin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AFP, check out the AFP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AFP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-alpha 1 Fetoprotein/AFP Antibody Picoband™ (A00522-3)
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Customer Reviews
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