Anti-Alpha SNAP/NAPA Antibody Picoband™

Product Name

Anti-Alpha SNAP/NAPA Antibody Picoband™

View all Napa Antibodies

SKU/Catalog Number

A05153-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-Alpha SNAP/NAPA Antibody Picoband™ catalog # A05153-1. Tested in WB, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-Alpha SNAP/NAPA Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05153-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Alpha SNAP/NAPA, which shares 95.7% amino acid (aa) sequence identity with mouse and rat NAPA.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05153-1 is reactive to NAPA in Human

Applications

A05153-1 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

33 kDa

Calculated molecular weight

33.233kDa

Background of Napa

N-ethylmaleimide-sensitive factor Attachment Protein Alpha, also known as SNAP-α, is a SNAP protein that is involved in the intra-cellular trafficking and fusing of vesicles to target membranes in cells. This gene encodes a member of the soluble NSF attachment protein (SNAP) family. SNAP proteins play a critical role in the docking and fusion of vesicles to target membranes as part of the 20S NSF-SNAP-SNARE complex. The encoded protein plays a role in the completion of membrane fusion by mediating the interaction of N-ethylmaleimide-sensitive factor (NSF) with the vesicle-associated and membrane-associated SNAP receptor (SNARE) complex, and stimulating the ATPase activity of NSF. Alternatively spliced transcript variants have been observed for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Alpha SNAP/NAPA using anti-Alpha SNAP/NAPA antibody (A05153-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human THP-1 whole cell lysates,
Lane 6: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Alpha SNAP/NAPA antigen affinity purified polyclonal antibody (Catalog # A05153-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Alpha SNAP/NAPA at approximately 33 kDa. The expected band size for Alpha SNAP/NAPA is at 33 kDa.

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Figure 2. Flow Cytometry analysis of 293T cells using anti-Alpha SNAP/NAPA antibody (A05153-1).
Overlay histogram showing 293T cells stained with A05153-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Alpha SNAP/NAPA Antibody (A05153-1, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For NAPA (Source: Uniprot.org, NCBI)

Alternative Names

alpha-SNAP; alpha-soluble NSF attachment protein; N-ethylmaleimide-sensitive factor attachment protein alpha; N-ethylmaleimide-sensitive factor attachment protein, alpha; S; SNAPA; SNAP-alpha NAPA SNAPA NSF attachment protein alpha alpha-soluble NSF attachment protein|N-ethylmaleimide-sensitive factor attachment protein, alpha|alpha-SNAP

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