Product Info Summary
SKU: | M06179 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M06179
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal Antibody catalog # M06179. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06179)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GDD-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human AP2M1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06179 is reactive to AP2M1 in Human, Mouse, Rat
Applications
M06179 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
34 kDa
Calculated molecular weight
49.655kDa
Background of AP2M1
Putative transcription factor involved in pancreas development and function.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of AP2M1 expression in (1) HEK293 cell lysate; (2) MCF-7 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For AP2M1 (Source: Uniprot.org, NCBI)
Gene Name
AP2M1
Full Name
AP-2 complex subunit mu
Weight
49.655kDa
Superfamily
adaptor complexes medium subunit family
Alternative Names
Adaptin-mu2; adaptor-related protein complex 2, mu 1 subunit; AP-2 mu chain; Clathrin assembly protein complex 2 medium chain; clathrin coat adaptor protein AP50; Clathrin coat assembly protein AP50; Clathrin coat-associated protein AP50; clathrin-associated/assembly/adaptor protein, medium 1; HA2 50 kDa subunit; KIAA0109; mu subunit; Plasma membrane adaptor AP-2 50 kDa protein; plasma membrane adaptor AP-2 50kDA protein AP2M1 AP50, CLAPM1, MRD60, mu2 adaptor related protein complex 2 subunit mu 1 AP-2 complex subunit mu|AP-2 mu 2 chain|HA2 50 kDA subunit|adaptin-mu2|adaptor protein complex AP-2 subunit mu|adaptor related protein complex 2 mu 1 subunit|adaptor-related protein complex 2 subunit mu|clathrin adaptor complex AP2, mu subunit|clathrin assembly protein complex 2 medium chain|clathrin assembly protein complex 2 mu medium chain|clathrin coat adaptor protein AP50|clathrin coat assembly protein AP50|clathrin coat-associated protein AP50|clathrin-associated/assembly/adaptor protein, medium 1|plasma membrane adaptor AP-2 50 kDa protein|plasma membrane adaptor AP-2 50kDA protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AP2M1, check out the AP2M1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AP2M1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal Antibody (M06179)
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No publications found for M06179
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal Antibody
Question
Would M06179 anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
As indicated on the product datasheet, M06179 anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-08
Question
I was wanting to use to test anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal antibody M06179 on rat erythroleukemia for research purposes, then I may be interested in using anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal antibody M06179 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
The products we sell, including anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal antibody M06179, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-09
Question
Is this M06179 anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal antibody reactive to the isotypes of AP2M1?
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
The immunogen of M06179 anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal antibody is A synthesized peptide derived from human AP2M1 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-12
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal antibody M06179. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-01
Question
I see that the anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal antibody M06179 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-10
Question
I have a question about product M06179, anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-05-11
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M06179 anti-AP2M1/Ap 2 Mu 1 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-05-11