Anti-Apc11 ANAPC11 Antibody

Product Name

Anti-Apc11 ANAPC11 Antibody

View all Apc11 Antibodies

SKU/Catalog Number

A08555

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Apc11 ANAPC11 Antibody (Catalog # A08555). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Apc11 ANAPC11 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08555)

Host

Rabbit

Contents

0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 76-84 of Human APC11 (C-terminal) coupled to KLH.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A08555 is reactive to ANAPC11 in Human

Applications

A08555 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

9.841kDa

Background of Apc11

APC11 is also known as Anaphase promoting complex subunit 11, APC11, Cyclosome subunit 11, Hepatocellular carcinoma associated RING finger protein, and HSPC214. APC11 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G₁ phase of the cell cycle. APC11 may function to recruit the E2 ubiquitin-conjugating enzymes to the complex. APC11 interacts with the cullin domain of ANAPC2 and also interacts with UBE2D2. APC11 shows both a cytoplasmic and nuclear localization. APC11 is expressed at high levels in skeletal muscle and heart; in moderate levels in brain, kidney, and liver; and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte. APC11 is a member of the RING-type zinc finger family and is auto-ubiquitinylated.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:10,000
IHC: User optimized
IP: User optimized
WB: 1:500 - 1:1,000

Validation Images & Assay Conditions

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Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.Data contributed by S.Jentsch.

Gene/Protein Information For ANAPC11 (Source: Uniprot.org, NCBI)

Alternative Names

anaphase promoting complex subunit 11 (yeast APC11 homolog); anaphase promoting complex subunit 11; anaphase-promoting complex subunit 11; APC11 anaphase promoting complex subunit 11 homolog; APC11Hepatocellular carcinoma-associated RING finger protein; Apc11p; Cyclosome subunit 11; HSPC214; MGC882 ANAPC11 APC11, Apc11p, HSPC214 anaphase promoting complex subunit 11 anaphase-promoting complex subunit 11|APC11 anaphase promoting complex subunit 11 homolog|anaphase promoting complex subunit 11 (yeast APC11 homolog)|cyclosome subunit 11|hepatocellular carcinoma-associated RING finger protein

For more info on ANAPC11, check out the ANAPC11 Infographic

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In this infographic, you will see the following information for ANAPC11: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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