Anti-APHC ACER3 Antibody

Boster Bio Anti-APHC ACER3 Antibody catalog # A10457-1. Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A10457-1
Size: 100μl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

Product Name

Anti-APHC ACER3 Antibody

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SKU/Catalog Number

A10457-1

Size

100μl

Form

Liquid

Description

Boster Bio Anti-APHC ACER3 Antibody catalog # A10457-1. Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-APHC ACER3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10457-1)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human APHC. at AA rangle: 140-220

Reactive Species

A10457-1 is reactive to ACER3 in Human, Mouse

Applications

A10457-1 is guaranteed for WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For ACER3 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9NUN7

Gene ID

55331

Gene Name

ACER3

Full Name

Alkaline ceramidase 3

Weight

31.552kDa

Superfamily

alkaline ceramidase family

Alternative Names

Alkaline CDase 3; alkaline ceramidase 3; Alkaline dihydroceramidase SB89; Alkaline phytoceramidase; aPHC; APHCAlkCDase 3; EC 3.5.1.-; FLJ11238; PHCA; phytoceramidase, alkaline ACER3 APHC, PHCA, PLDECO alkaline ceramidase 3 alkaline ceramidase 3|alkCDase 3|alkaline CDase 3|alkaline dihydroceramidase SB89|alkaline phytoceramidase|phytoceramidase, alkaline

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ACER3, check out the ACER3 Infographic

ACER3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ACER3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Anti-APHC ACER3 Antibody

Question

We are currently using anti-APHC antibody A10457-1 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2020-03-26

Answer

The anti-APHC antibody (A10457-1) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-03-26

Question

See attached the WB image, lot number and protocol we used for eye using anti-APHC antibody A10457-1. Please let me know if you require anything else.

F. Gonzalez

Verified customer

Asked: 2020-03-04

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-04

Question

We need to test anti-APHC antibody A10457-1 on human eye for research purposes, then I may be interested in using anti-APHC antibody A10457-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-07-23

Answer

The products we sell, including anti-APHC antibody A10457-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-07-23

Question

I have a question about product A10457-1, anti-APHC antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

A. Roberts

Verified customer

Asked: 2019-07-04

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10457-1 anti-APHC antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-04

Question

I was wanting to use your anti-APHC antibody for WB for human eye on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human eye identification?

Verified Customer

Verified customer

Asked: 2019-06-06

Answer

You can see on the product datasheet, A10457-1 anti-APHC antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human eye in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-06

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for eye using anti-APHC antibody A10457-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-10-24

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-10-24

Question

Would anti-APHC antibody A10457-1 work for WB with eye?

Verified Customer

Verified customer

Asked: 2017-10-09

Answer

According to the expression profile of eye, ACER3 is highly expressed in eye. So, it is likely that anti-APHC antibody A10457-1 will work for WB with eye.

Boster Scientific Support

Answered: 2017-10-09

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
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A10457-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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