Product Info Summary
SKU: | A05155 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-ASCL2/Mash2 Antibody
View all ASCL2/Mash2 Antibodies
SKU/Catalog Number
A05155
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-ASCL2/Mash2 Antibody (Catalog # A05155). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ASCL2/Mash2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05155)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from Internal of human ASCL2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05155 is reactive to ASCL2 in Human
Applications
A05155 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
111 kDa
Calculated molecular weight
20.185kDa
Background of ASCL2/Mash2
AS-C proteins are involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system.
Alders M., Hum. Mol. Genet. 6:859-867(1997).
Westerman B.A., Placenta 22:511-518(2001).
Miyamoto T., Cytogenet. Cell Genet. 73:312-314(1996).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from K562 cells, using ASCL2 antibody A05155.
Protein Target Info & Infographic
Gene/Protein Information For ASCL2 (Source: Uniprot.org, NCBI)
Gene Name
ASCL2
Full Name
Achaete-scute homolog 2
Weight
20.185kDa
Alternative Names
achaete-scute complex (Drosophila) homolog-like 2; achaete-scute complex homolog 2 (Drosophila); achaete-scute complex-like 2 (Drosophila); ASCL2; ASH2; ASH-2; BHLHA45; bHLHa45achaete-scute homolog 2; Class A basic helix-loop-helix protein 45; HASH2; HASH2achaete-scute complex-like 2; mammalian achaete/scute homologue 2; Mash2 ASCL2 ASH2, HASH2, MASH2, bHLHa45 achaete-scute family bHLH transcription factor 2 achaete-scute homolog 2|achaete-scute complex homolog 2|achaete-scute complex-like 2|class A basic helix-loop-helix protein 45|mammalian achaete/scute homologue 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ASCL2, check out the ASCL2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ASCL2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ASCL2/Mash2 Antibody (A05155)
Hello CJ!
No publications found for A05155
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-ASCL2/Mash2 Antibody
Question
I see that the anti-ASCL2/Mash2 antibody A05155 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-21
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-21
Question
Is a blocking peptide available for product anti-ASCL2/Mash2 antibody (A05155)?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
We do provide the blocking peptide for product anti-ASCL2/Mash2 antibody (A05155). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-23
Question
Please see the WB image, lot number and protocol we used for parotid gland using anti-ASCL2/Mash2 antibody A05155. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-07
Question
Would anti-ASCL2/Mash2 antibody A05155 work on primate WB with parotid gland?
T. Baker
Verified customer
Asked: 2018-09-03
Answer
Our lab technicians have not tested anti-ASCL2/Mash2 antibody A05155 on primate. You can run a BLAST between primate and the immunogen sequence of anti-ASCL2/Mash2 antibody A05155 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate parotid gland in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-03
Question
We are currently using anti-ASCL2/Mash2 antibody A05155 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2018-02-08
Answer
The anti-ASCL2/Mash2 antibody (A05155) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for parotid gland using anti-ASCL2/Mash2 antibody A05155. Let me know if you need anything else.
V. Mitchell
Verified customer
Asked: 2016-04-25
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-04-25
Question
I was wanting to use your anti-ASCL2/Mash2 antibody for WB for human parotid gland on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human parotid gland identification?
M. Parker
Verified customer
Asked: 2013-07-19
Answer
As indicated on the product datasheet, A05155 anti-ASCL2/Mash2 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human parotid gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-07-19