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Product Info Summary
| SKU: | A00371-3 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ATF4 Antibody
SKU/Catalog Number
A00371-3
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ATF4 Antibody catalog # A00371-3. Tested in WB,ICC/IF,IHC,IP,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATF4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00371-3)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human NOS2 around the phosphorylation site of Y151.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with eNOS or nNOS.
Reactive Species
A00371-3 is reactive to ATF4 in Human, Mouse, Rat
Applications
A00371-3 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
38.59kDa
Background of ATF4
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
David A. Jans, J. Biol. Chem., Nov 1996; 271: 30781.
Eugene Estella, Diabetes, Aug 2006; 55: 2212 - 2219.
Yong Gu, J. Biol. Chem., May 1996; 271: 10816.
Jiuru Sun, J. Biol. Chem., Nov 1996; 271: 27802.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
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Figure 2. Immunocytochemistry staining of ATF4 using Anti-ATF4 Antibody (A00371-3).
ICC staining ATF4 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 1. Western blotting validation for Anti-ATF4 Antibody A00371-3
Western blot analysis of ATF4 on different lysates using anti-ATF4 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For ATF4 (Source: Uniprot.org, NCBI)
Gene Name
ATF4
Full Name
Cyclic AMP-dependent transcription factor ATF-4
Weight
38.59kDa
Superfamily
bZIP family
Alternative Names
activating transcription factor 4 (tax-responsive enhancer element B67); Activating transcription factor 4; ATF4; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB-2DNA-binding protein TAXREB67; cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; TAXREB67; TAXREB67CREB2cAMP response element-binding protein 2; Tax-responsive enhancer element-binding protein 67; TXREB ATF4 CREB-2, CREB2, TAXREB67, TXREB activating transcription factor 4 cyclic AMP-dependent transcription factor ATF-4|DNA-binding protein TAXREB67|cAMP response element-binding protein 2|cAMP-dependent transcription factor ATF-4|cAMP-responsive element-binding protein 2|cyclic AMP-responsive element-binding protein 2|tax-responsive enhancer element B67|tax-responsive enhancer element-binding protein 67
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATF4, check out the ATF4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATF4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATF4 Antibody (A00371-3)
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No publications found for A00371-3
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