Product Info Summary
SKU: | A00655-1 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-ATF6 Antibody
SKU/Catalog Number
A00655-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ATF6 Antibody (Catalog # A00655-1). Tested in ELISA, WB, IHC-P, ICC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
ATF6 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ATF6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00655-1)
Host
Rabbit
Contents
ATF6 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ATF6 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ATF6. The immunogen is located within amino acids 470 - 520 of ATF6.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
JAG1 antibody is human, mouse and rat reactive. At least two isoforms of JAG1 are known to exist; this antibody will detect both isoforms.
Reactive Species
A00655-1 is reactive to ATF6 in Human, Mouse
Applications
A00655-1 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
74.585kDa
Background of ATF6
Disruptions of protein folding and maturation in the endoplasmic reticulum (ER) result in the activation of the unfolded protein response (UPR), an integrated cellular signaling pathway that transmits information from the ER lumen to the cytoplasm and nucleus. Activating transcription factor 6 (ATF6) as well as the ER-transmembrane protein kinases IRE1p and PERK are the major transducers of the UPR. ATF6 is an ER transmembrane protein that is normally bound to the ER chaperone GRP78, but upon ER stress is released from GRP78 and proteolytically cleaved to yield a cytosolic fragment which then migrates to the nucleus, and together with the transcription factor XBP-1, activates transcription of UPR-responsive genes. ATF6 has two isoforms (ATF6α and ATF6β); only ATF6α is recognized by this antibody.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ATF6 antibody can be used for the detection of ATF6 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ATF6 in EL4 cell lysate with ATF6 antibody at (A) 0.5 and (B) 1 μg/mL.
Click image to see more details
Immunocytochemical staining of MCF7 cells using ATF6 antibody at 10 μg/mL.
Click image to see more details
Immunofluorescence of ATF6 in MCF7 cells with ATF6 antibody at 10 μg/mL.
Click image to see more details
Immunohistochemistry of ATF6 in human breast tissue with ATF6 antibody at 5 μg/ml.
Click image to see more details
Immunofluorescence of ATF6 in human breast tissue with ATF6 antibody at 20 μg/ml.
Green: ATF6 Antibody (A00655-1)
Blue: DAPI staining
Click image to see more details
Immunohistochemistry of ATF6 in human pancreas tissue with ATF6 antibody at 5 μg/ml.
Click image to see more details
Immunofluorescence of ATF6 in human pancreas tissue with ATF6 antibody at 20 μg/ml.
Green: ATF6 Antibody (A00655-1)
Blue: DAPI staining
Protein Target Info & Infographic
Gene/Protein Information For ATF6 (Source: Uniprot.org, NCBI)
Gene Name
ATF6
Full Name
Cyclic AMP-dependent transcription factor ATF-6 alpha
Weight
74.585kDa
Superfamily
bZIP family
Alternative Names
ACHM7; Activating transcription factor 6 alpha; activating transcription factor 6; atf6 a; ATF6 alpha; ATF6; ATF6A; ATF6-alpha; cAMP-dependent transcription factor ATF-6 alpha; cyclic AMP-dependent transcription factor ATF-6 alpha ATF6 ACHM7A, ATF6 activating transcription factor 6 cyclic AMP-dependent transcription factor ATF-6 alpha|cAMP-dependent transcription factor ATF-6 alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATF6, check out the ATF6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATF6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATF6 Antibody (A00655-1)
Hello CJ!
No publications found for A00655-1
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-ATF6 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-ATF6 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question