Anti-BDNF Antibody Picoband™

Product Name

Anti-BDNF Antibody Picoband™

View all Bdnf Antibodies

SKU/Catalog Number

PB9075

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BDNF Antibody Picoband™ catalog # PB9075. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BDNF Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9075)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

Anti-BDNF Picoband™ Antibody (PB9075) was raised against E.coli-derived human BDNF recombinant protein (Position: H129-R247). Human BDNF shares 100% amino acid (aa) sequence identity with both mouse and rat BDNF.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9075 is reactive to BDNF in Human, Mouse, Rat

Applications

PB9075 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

28 kDa

Calculated molecular weight

27.818kDa

Background of Bdnf

Brain-derived neurotrophic factor, also known as BDNF, is a secreted protein that, in humans, is encoded by the BDNF gene. BDNF is a member of the neurotrophin family of growth factors, which are related to the canonical nerve growth factor. It is mapped to 11p14.1. BDNF is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is expressed within peripheral ganglia and is not restricted to neuronal target fields. BDNF has been purified and shown to reduce the amount of naturally occurring neuronal cell death in portions of the peripheral nervous system.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, Human, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x10⁶ cells, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. IHC analysis of BDNF using anti-BDNF antibody (PB9075).
BDNF was detected in paraffin-embedded section of Rat Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BDNF Antibody (PB9075) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 2. IHC analysis of BDNF using anti-BDNF antibody (PB9075).
BDNF was detected in paraffin-embedded section of Mouse Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-BDNF Antibody (PB9075) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 3. Western blot analysis of BDNF using anti-BDNF antibody (PB9075).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Brain Tissue Lysate
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BDNF antigen affinity purified polyclonal antibody (Catalog # PB9075) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BDNF at approximately 28KD. The expected band size for BDNF is at 28KD.

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Figure 4. Flow Cytometry analysis of U-87 cells using anti-BDNF antibody (PB9075).
Overlay histogram showing U-87 cells stained with PB9075 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BDNF Antibody (PB9075,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For BDNF (Source: Uniprot.org, NCBI)

Alternative Names

Abrineurin; ANON2; BDNF; brain-derived neurotrophic factor; BULN2; MGC34632; Neurotrophin BDNF ANON2, BULN2 brain derived neurotrophic factor brain-derived neurotrophic factor|abrineurin|neurotrophin

For more info on BDNF, check out the BDNF Infographic

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In this infographic, you will see the following information for BDNF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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