Click image to see more details
Product Info Summary
| SKU: | M00327 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Beclin 1 BECN1 Monoclonal Antibody
SKU/Catalog Number
M00327
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Beclin 1 BECN1 Monoclonal Antibody catalog # M00327. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Beclin 1 BECN1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00327)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADA-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Beclin 1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00327 is reactive to BECN1 in Human, Mouse, Rat
Applications
M00327 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
47 kDa
Calculated molecular weight
51.896kDa
Background of Beclin 1
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of Beclin 1 expression in (1) HEK293 cell lysate; (2) NIH/3T3 cell lysate; (3) C6 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human breast cancer, using Beclin 1 Antibody.
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Immunofluorescent analysis of Hela cells, using Beclin 1 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For BECN1 (Source: Uniprot.org, NCBI)
Gene Name
BECN1
Full Name
Beclin-1
Weight
51.896kDa
Superfamily
beclin family
Alternative Names
ATG6 autophagy related 6 homolog; ATG6; beclin 1 (coiled-coil, moesin-like BCL2 interacting protein); beclin 1 (coiled-coil, moesin-like BCL2-interacting protein); Beclin 1; beclin 1, autophagy related; beclin1; beclin-1; BECN1; Coiled-coil myosin-like BCL2-interacting protein; GT197; Protein GT197; VPS30 BECN1 ATG6, VPS30, beclin1 beclin 1 beclin-1|ATG6 autophagy related 6 homolog|beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)|beclin 1, autophagy related|coiled-coil myosin-like BCL2-interacting protein|testis secretory sperm-binding protein Li 215e
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BECN1, check out the BECN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BECN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Beclin 1 BECN1 Monoclonal Antibody (M00327)
Hello CJ!
M00327 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ni K,Hua Y.Hydrogen sulfide exacerbated periodontal inflammation and induced autophagy in experimental periodontitis.Int Immunopharmacol.2021 Jan 30;93:107399.doi:10.1016/j.intimp.2021.107399.Epub ahead of print.PMID:33529908.
Species: Human,Rat
M00327 usage in article: APP:IF, SAMPLE:ALVEOLAR BONE SPECIMEN, DILUTION:1:500
Song L,Yao L,Zhang L,Piao Z,Lu Y.Schizandrol A protects against Aβ1-42-induced autophagy via activation of PI3K/AKT/mTOR pathway in SH-SY5Y cells and primary hippocampal neurons.Naunyn Schmiedebergs Arch Pharmacol.2020 Sep;393(9):1739-1752.doi:10.1007/s00
Species: Human
M00327 usage in article: APP:WB, SAMPLE:SH-SY5Y CELLS, DILUTION:1:400
Bifidobacterial recombinant thymidine kinase-ganciclovir gene therapy system induces FasL and TNFR2 mediated antitumor apoptosis in solid tumors
Recombinant Newcastle disease virus (rL-RVG) triggers autophagy and apoptosis in gastric carcinoma cells by inducing ER stress
Song J, Hu Y, Li J, Zheng H, Wang J, Guo L, Shi H, Liu L. Arch Virol. 2018 Jan;163(1):135-144. doi: 10.1007/s00705-017-3592-x. Epub 2017 Oct 19. Suppression of the toll-like receptor 7-dependent type I interferon production pathway by autophagy re...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-Beclin 1 BECN1 Monoclonal Antibody
Question
We purchased anti-Beclin 1 Monoclonal antibody for ICC on mammary gland in the past. I am using human, and We are going to use the antibody for IP next. you antibody examining mammary gland as well as liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for IP?
Verified Customer
Verified customer
Asked: 2020-03-30
Answer
I looked at the website and datasheets of our anti-Beclin 1 Monoclonal antibody and it appears that M00327 has been tested on human in both ICC and IP. Thus M00327 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-30
Question
Our lab want to know about using your anti-Beclin 1 Monoclonal antibody for negative regulation of autophagosome assembly studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
Thanks for your inquiry. This M00327 anti-Beclin 1 Monoclonal antibody is tested on hela cells. It is guaranteed to work for IP, IF, IHC, ICC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-08
Question
My lab would like to test anti-Beclin 1 Monoclonal antibody M00327 on human brain for research purposes, then I may be interested in using anti-Beclin 1 Monoclonal antibody M00327 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
The products we sell, including anti-Beclin 1 Monoclonal antibody M00327, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-08
Question
I see that the anti-Beclin 1 Monoclonal antibody M00327 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-31
Question
Does M00327 anti-Beclin 1 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
As indicated on the product datasheet, M00327 anti-Beclin 1 Monoclonal antibody as been tested on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-25
Question
I was wanting to use your anti-Beclin 1 Monoclonal antibody for ICC for human brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
As indicated on the product datasheet, M00327 anti-Beclin 1 Monoclonal antibody has been tested for IP, IF, IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-26
Question
Is this M00327 anti-Beclin 1 Monoclonal antibody reactive to the isotypes of BECN1?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
The immunogen of M00327 anti-Beclin 1 Monoclonal antibody is A synthesized peptide derived from human Beclin 1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-28
Question
Our lab were satisfied with the WB result of your anti-Beclin 1 Monoclonal antibody. However we have observed positive staining in cervix carcinoma cytoplasm using this antibody. Is that expected? Could you tell me where is BECN1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-10-10
Answer
According to literature, cervix carcinoma does express BECN1. Generally BECN1 expresses in cytoplasm. Regarding which tissues have BECN1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9765397, 10395800
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 14702039
Mammary gland, Pubmed ID: 7490091
Boster Scientific Support
Answered: 2018-10-10
Question
Can you help my question with product M00327, anti-Beclin 1 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-07-23
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00327 anti-Beclin 1 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-23
Question
Is there a BSA free version of anti-Beclin 1 Monoclonal antibody M00327 available?
T. Mangal
Verified customer
Asked: 2018-04-20
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Beclin 1 Monoclonal antibody M00327 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-04-20
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-Beclin 1 Monoclonal antibody M00327. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-04-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-04-17
Question
Is a blocking peptide available for product anti-Beclin 1 Monoclonal antibody (M00327)?
Verified Customer
Verified customer
Asked: 2018-02-05
Answer
We do provide the blocking peptide for product anti-Beclin 1 Monoclonal antibody (M00327). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-02-05
Question
I have attached the WB image, lot number and protocol we used for brain using anti-Beclin 1 Monoclonal antibody M00327. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-01-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-01
Question
We are currently using anti-Beclin 1 Monoclonal antibody M00327 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
V. Krishna
Verified customer
Asked: 2017-07-14
Answer
The anti-Beclin 1 Monoclonal antibody (M00327) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-14
Question
Will anti-Beclin 1 Monoclonal antibody M00327 work for ICC with brain?
G. Bhatt
Verified customer
Asked: 2015-06-30
Answer
According to the expression profile of brain, BECN1 is highly expressed in brain. So, it is likely that anti-Beclin 1 Monoclonal antibody M00327 will work for ICC with brain.
Boster Scientific Support
Answered: 2015-06-30
Question
We have observed staining in rat brain. Do you have any suggestions? Is anti-Beclin 1 Monoclonal antibody supposed to stain brain positively?
P. Rodriguez
Verified customer
Asked: 2013-11-21
Answer
From what I have seen in literature brain does express BECN1. From what I have seen in Uniprot.org, BECN1 is expressed in frontal cortex, brain, liver, cervix, mammary gland, cervix carcinoma, erythroleukemia, among other tissues. Regarding which tissues have BECN1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9765397, 10395800
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 14702039
Mammary gland, Pubmed ID: 7490091
Boster Scientific Support
Answered: 2013-11-21
