Anti-Beta Amyloid APP Antibody

Product Name

Anti-Beta Amyloid APP Antibody

View all APP Antibodies

SKU/Catalog Number

A00081-2

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Beta Amyloid APP Antibody (Catalog # A00081-2). Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Beta Amyloid APP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00081-2)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This antibody was affinity purified from whole rabbit serum prepared by repeated immunizations with a synthetic peptide corresponding to an extracellular region of human beta amyloid conjugated to KLH using maleimide.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A00081-2 is reactive to APP in Human, Mouse

Applications

A00081-2 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

86.943kDa

Background of APP

Beta amyloid, often abbreviated as A-beta, is a protein that builds up in the brains of persons with Alzheimer's disease, collecting in clumps called plaques or senile plaques. While some researchers question whether beta amyloid is the cause of the dementia, most agree that it is involved in the disruption of thinking that is a hallmark of the disease. In some cases of familial Alzheimer's disease, mutations in genes for the proteins called the presenilins lead to increased production of amyloid. Researchers have been looking at how presenilin-1 in particular contributes to the excess buildup of beta amyloid. Presenilin-1 apparently acts to increase the activity of gamma-secretase, an enzyme that changes a normal protein (amyloid precursor protein or APP) into beta amyloid itself. Furthermore, presenilin-1 might be gamma-secretase. Anti-Beta Amyloid Antibody is useful for researchers interested in TLR signaling and Alzheimer's research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:30,000
IHC: 1:50-1:200
IF Microscopy: 1:50-1:200
WB: 1:1,000-1:5000

Validation Images & Assay Conditions

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Beta Amyloid was detected in immunohistochemical frozen sections of TG APP23 mouse brain cortex tissues using rabbit anti-Beta Amyloid affinity purified polyclonal antibody (Catalog # A00081-2) at 1:200. Carl Hobbs, King`s College London, United Kingdom.

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Beta Amyloid was detected in paraffin-embedded sections of human heart tissues using rabbit anti-Beta Amyloid affinity purified polyclonal antibody (Catalog # A00081-2) at 5

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Western blot analysis of Beta Amyloid expression in HEK293 whole cell lysates (lane 1), mouse brain whole cell lysates (lane 2) and A-172 whole cell lysates (lane 3). Beta Amyloid at 40 kDa was detected using rabbit anti-Beta Amyloid affinity purified polyclonal antibody (Catalog # A00081-2) at 1:1,000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

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ELISA results of purified Rabbit anti-Beta Amyloid Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1

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Western blot analysis of Beta Amyloid expression in mouse brain extract (lane 1) and mouse spinal chord (lane 2). A 40-50 kDa band consistent with a higher MW precursor form of beta amyloid was detected using rabbit anti-Beta Amyloid affinity purified polyclonal antibody (Catalog # A00081-2) at 1:1000. A secondary Ab only control (Shown right) showed no detectable signal.

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Western blot analysis of Beta Amyloid expression in HEK293 whole cell lysates (lane 1), HeLa Lysate whole cell lysates (lane 2), MCF-7 cell lysates (lane 3), Jurkat cell lysates (lane 4), A431 cell lysates (lane 5), LNCaP cell lysates (lane 6), A-172 cell lysates (lane 7) and NIH/3T3 cell lysates (lane 8). Beta Amyloid at 40 kDa was detected using rabbit anti-Beta Amyloid affinity purified polyclonal antibody (Catalog # A00081-2) at 1:5,000. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).

Gene/Protein Information For APP (Source: Uniprot.org, NCBI)

Alternative Names

Abeta; Amyloid beta; beta-Amyloid Peptide APP AAA, ABETA, ABPP, AD1I, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4, APP amyloid beta precursor protein amyloid-beta precursor protein|alzheimer disease amyloid protein|amyloid beta (A4) precursor protein|amyloid beta A4 protein|amyloid precursor protein|beta-amyloid peptide|beta-amyloid peptide(1-40)|beta-amyloid peptide(1-42)|beta-amyloid precursor protein|cerebral vascular amyloid peptide|peptidase nexin-II|protease nexin-II|testicular tissue protein Li 2

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In this infographic, you will see the following information for APP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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