Click image to see more details
Product Info Summary
| SKU: | A04792 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Bif SH3GLB1 Antibody
View all Endophilin B1/Bif-1 Antibodies
SKU/Catalog Number
A04792
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Bif SH3GLB1 Antibody (Catalog # A04792). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Bif antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Bif SH3GLB1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04792)
Host
Rabbit
Contents
Bif Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
BIF antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human BIF. The immunogen is located within the last 50 amino acids of Bif.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms are known to exist. This antibody will recognize both isoforms. POU3F2 antibody is predicted to not cross-react with other members of the POU domain class 3 family.
Reactive Species
A04792 is reactive to SH3GLB1 in Human, Mouse
Applications
A04792 is guaranteed for ELISA, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
40.796kDa
Background of Endophilin B1/Bif-1
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells and is caused by the activation of proteolytic enzymes termed caspases. Proteins that comprise the Bcl-2 family such as Bax appear to control the activation of these enzymes. Bax activity was found to be regulated by its association with Bax-interacting factor 1 (BIF), a member of the endophilin B family that is associated with intracellular membranes. Following this interaction, Bax undergoes a conformational change and translocates to mitochondrial membranes. The Bax/BIF interaction appears to be enhanced by apoptotic stimuli, suggesting that BIF acts as the trigger to activate Bax, and as suppression of BIF promoted HeLa cell colony formation in soft agar, it may have a role in the suppression of cancer progression. At least two isoforms of BIF are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
Submit A Review
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
BIF antibody can be used for the detection of BIF by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of BIF in HeLa cell lysate with BIF antibody at (A) 1 and (B) 2 μg/mL.
Click image to see more details
Immunocytochemistry of BIF in HeLa with BIF antibody at 10 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For SH3GLB1 (Source: Uniprot.org, NCBI)
Gene Name
SH3GLB1
Full Name
Endophilin-B1
Weight
40.796kDa
Superfamily
endophilin family
Alternative Names
Bax-interacting factor 1; Bif1; Bif-1; Bif-1dJ612B15.2; CGI-61; Endophilin B1; endophilin-B1; KIAA0491endophilin B1; SH3 domain-containing GRB2-like protein B1; SH3-containing protein SH3GLB1; SH3-domain GRB2-like endophilin B1; SH3-domain, GRB2-like, endophilin B1; SH3GLB1 SH3GLB1 Bif-1, CGI-61, PPP1R70, dJ612B15.2 SH3 domain containing GRB2 like, endophilin B1 endophilin-B1|Bax-interacting factor 1|SH3 domain-containing GRB2-like protein B1|SH3-containing protein SH3GLB1|SH3-domain GRB2 like endophilin B1|protein phosphatase 1, regulatory subunit 70|testicular tissue protein Li 172
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SH3GLB1, check out the SH3GLB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SH3GLB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bif SH3GLB1 Antibody (A04792)
Hello CJ!
No publications found for A04792
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Bif SH3GLB1 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
Be the first to review Anti-Bif SH3GLB1 Antibody
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
