Anti-Caspase-9 CASP9 Antibody

Product Name

Anti-Caspase-9 CASP9 Antibody

View all Caspase-9 Antibodies

SKU/Catalog Number

A00080-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Caspase-9 CASP9 Antibody (Catalog # A00080-1). Tested in ELISA, WB, ICC, IF, IP applications. This antibody reacts with Human, Mouse.

Storage & Handling

Caspase-9 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Caspase-9 CASP9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00080-1)

Host

Rabbit

Contents

Caspase-9 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Anti-Caspase 9 antibody was raised against a peptide corresponding to 20 amino acids near the center of human Caspase 9. The immunogen is located within amino acids 290-340 of Caspase 9.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Caspase-9 antibody is predicted to have no cross reactivity to other members in the caspase family.

Reactive Species

A00080-1 is reactive to CASP9 in Human, Mouse

Applications

A00080-1 is guaranteed for ELISA, IP, IF, ICC, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

46.281kDa

Background of Caspase-9

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1 μg/mL; ICC: 2- 5 μg/mL; IF: 5-20 μg/mL.
Antibody validated: Western Blot in human and mouse samples, Immunocytochemistry, Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

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Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: Caspase 9, (1 μg/mL), Caspase 9, A00080-1 (1 μg/mL) and beta-actin (1.5 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Western Blot Validation in Human Cell Lines
Loading: 15 μg of lysates per lane.Antibodies: : Caspase 9, A00080-1 (1 μg/mL)), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Western Blot Validation in Mouse Cell Line
Loading: 15 μg of 3T3/NIH cell lysate. Antibodies: : Caspase 9, A00080-1 (1 μg/mL)), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Immunofluorescence Validation of Caspase 9 in K562 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Caspase 9 with A00080-1 at 20 μg/mL

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Immunofluorescence Validation of Caspase 9 in HeLa Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Caspase 9 with A00080-1 at 5 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

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Immunocytochemistry Validation of Caspase 9 in HeLa Cells
Immunocytochemical analysis of HeLa cells using anti-Caspase 9 antibody (A00080-1) at 5 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Gene/Protein Information For CASP9 (Source: Uniprot.org, NCBI)

Alternative Names

APAF-3APAF3; apoptotic protease activating factor 3; Apoptotic protease Mch-6; Apoptotic protease-activating factor 3; CASP9; CASP-9; caspase 9, apoptosis-related cysteine peptidase; Caspase9; Caspase-9; EC 3.4.22.62; ICE-LAP6CASPASE-9c; ICE-like apoptotic protease 6; MCH6apoptosis-related cysteine protease CASP9 APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56 caspase 9 caspase-9|ICE-like apoptotic protease 6|apoptotic protease MCH-6|apoptotic protease activating factor 3|caspase 9, apoptosis-related cysteine peptidase|protein phosphatase 1, regulatory subunit 56

For more info on CASP9, check out the CASP9 Infographic

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In this infographic, you will see the following information for CASP9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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