Anti-CD14 Antibody Picoband™

CD14 antibody

Boster Bio Anti-CD14 Antibody Picoband™ catalog # A00137-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC applications. This antibody reacts with Human. Cited in 2 publication(s).

Product Info Summary

SKU: A00137-2
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: ELISA, Flow Cytometry, IF, IHC, ICC

Product Name

Anti-CD14 Antibody Picoband™

View all CD14 Antibodies

SKU/Catalog Number

A00137-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CD14 Antibody Picoband™ catalog # A00137-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-CD14 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00137-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CD14 recombinant protein (Position: T20-N259).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00137-2 is reactive to CD14 in Human

Applications

A00137-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC Boster Guarantee

Observed Molecular Weight

50 kDa

Calculated molecular weight

40.076kDa

Background of CD14

CD14 is a single-copy gene encoding 2 protein forms: a 50- to 55-kD glycosylphosphatidylinositol-anchored membrane protein (mCD14) and a monocyte or liver-derived soluble serum protein (sCD14) that lacks the anchor. This gene is located at bands 5q23-q31. The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry, 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CD14 (Source: Uniprot.org, NCBI)

Gene Name

CD14

Full Name

Monocyte differentiation antigen CD14

Weight

40.076kDa

Alternative Names

CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein CD14 CD14 molecule monocyte differentiation antigen CD14|myeloid cell-specific leucine-rich glycoprotein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CD14, check out the CD14 Infographic

CD14 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CD14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00137-2 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Expression and clinical significance of extracellular matrix metalloproteinase inducer, EMMPRIN/CD147, in human osteosarcoma

Enhanced expression of the human CD14 protein in tobacco using a 22-kDa alpha-zein signal peptide

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Order DetailsPrice
A00137-2

100μg

$370
A00137-2-10ug

10μg sample (liquid)

$99
A00137-2-Biotin

100 μg Biotin conjugated

$570
A00137-2-Cy3

100 μg Cy3 conjugated

$570
A00137-2-Dylight488

100 μg Dylight488 conjugated

$570
A00137-2-Dylight550

100 μg Dylight550 conjugated

$570
A00137-2-Dylight594

100 μg Dylight594 conjugated

$570
A00137-2-FITC

100 μg FITC conjugated

$570
A00137-2-HRP

100 μg HRP conjugated

$570
A00137-2-APC

100 μg APC conjugated

$670
A00137-2-PE

100 μg PE conjugated

$670
A00137-2-carrier-free

Carrier Free

$370

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A00137-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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