Anti-CD54 / ICAM-1 Monoclonal Antibody

Product Name

Anti-CD54 / ICAM-1 Monoclonal Antibody

View all ICAM-1/CD54 Antibodies

SKU/Catalog Number

M00171

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-CD54 / ICAM-1 Monoclonal Antibody (Catalog # M00171). Tested in Functional Assay, Flow Cytometry, IHC, IF applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-CD54 / ICAM-1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00171)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: W-CAM-1; same as Wehi-CAM-1 or 1H4

Isotype

IgG2b, kappa

Immunogen

Raji Burkitt lymphoma cells

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00171 is reactive to ICAM1 in Human

Applications

M00171 is guaranteed for Flow Cytometry, IF, IHC, Functional Assay Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

57.825kDa

Background of ICAM-1/CD54

Recognizes an 85-115kDa protein (variation with cell type), identified as intercellular adhesion molecule (ICAM-1) (Workshop IV). It has 7 potential N-linked glycosylation sites. ICAM-1 is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1). This interaction enhances antigen-specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected RBCs. W-CAM-1 MAb blocks aggregation of cell lines mediated by the ICAM-1 and blocks homotypic binding of Purified populations of activated T- and B-lymphocytes and also aggregation of mixed T- and B-cell blasts. It inhibits T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T-cell mediated B-cell activation) is significantly inhibited. This monoclonal antibody blocks elements of both effector arms of immune system (cytotoxic cell function and Ig production).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Functional Studies (Order Ab without BSA & Azide)
Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Melanoma stained with Anti-CD54 Monoclonal Antibody (W-CAM-1).

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SDS-PAGE analysis purified Anti-CD54 Monoclonal Antibody (W-CAM-1). Confirmation of integrity and purity of antibody.

Gene/Protein Information For ICAM1 (Source: Uniprot.org, NCBI)

Alternative Names

BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58 ICAM1 BB2, CD54, P3.58 intercellular adhesion molecule 1 intercellular adhesion molecule 1|ICAM-1|cell surface glycoprotein P3.58|epididymis secretory sperm binding protein|intercellular adhesion molecule 1 (CD54), human rhinovirus receptor|major group rhinovirus receptor

For more info on ICAM1, check out the ICAM1 Infographic

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In this infographic, you will see the following information for ICAM1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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