Anti-CDK4 Antibody (Monoclonal, DCS-31)

Product Name

Anti-CDK4 Antibody (Monoclonal, DCS-31)

View all CDK4 Antibodies

SKU/Catalog Number

MA1020

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CDK4 Antibody (Monoclonal, DCS-31) catalog # MA1020. Tested in ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CDK4 Antibody (Monoclonal, DCS-31) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1020)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

DCS-31

Isotype

Mouse IgG2a

Immunogen

Recombinant human Cdk4 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1020 is reactive to CDK4 in Human, Mouse, Rat

Applications

MA1020 is guaranteed for ICC, WB Boster Guarantee

Observed Molecular Weight

34 kDa

Calculated molecular weight

33.73kDa

Background of CDK4

Cyclin-dependent kinase-4 (CDK4) is a protein-serine kinase involved in the cell cycle. Human cell division is regulated primarily at the G1-to-S or the G2-to-M boundaries within the cell cycle. The complexes formed by CDK4 and the D-type cyclins are involved in the control of cell proliferation during the G1 phase. CDK4 is inhibited by p16, also known as cyclin-dependent kinase inhibitor-2. CDK4 is mapped to 12q14. CDK4 expression and activity are required for cytokine responsiveness in T cells.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Western blot, 0.5-1μg/ml, Human, mouse, rat

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of CDK4 using anti-CDK4 antibody (MA1020).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: rat PC-12 whole cell lysates,
Lane 4: mosue NIH/3T3 whole cell lysates,
Lane 5: mosue RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CDK4 antigen affinity purified monoclonal antibody (Catalog # MA1020) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CDK4 at approximately 34 kDa. The expected band size for CDK4 is at 34 kDa.

Gene/Protein Information For CDK4 (Source: Uniprot.org, NCBI)

Alternative Names

CDK4; Cell division protein kinase 4; CMM3; cyclin-dependent kinase 4; EC 2.7.11; EC 2.7.11.22; melanoma cutaneous malignant, 3; MGC14458; PSK-J3; PSK-J3cell division kinase 4 CDK4 CMM3, PSK-J3 cyclin dependent kinase 4 cyclin-dependent kinase 4|cell division protein kinase 4

For more info on CDK4, check out the CDK4 Infographic

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In this infographic, you will see the following information for CDK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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