|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-CDK6 Antibody (Monoclonal, DCS-90)|
|Description||Mouse IgG monoclonal antibody for CDK6, cyclin-dependent kinase 6 (CDK6) detection. Tested with WB, ICC in Human;mouse;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-CDK6 Antibody (Monoclonal, DCS-90) DCS-90 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1021)|
|Replacement Item||This antibody may replace the following items: sc-32501|sc-7180|sc-177|sc-56362|sc-7181|sc-271364|sc-56282|sc-53638|sc-7961|sc-390493|sc-177-G from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||Recombinant human Cdk6 protein.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.|
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Cyclin-dependent kinase 6|
|Molecular Weight||36952 MW|
|Protein Function||CDK6: Serine/threonine-protein kinase involved in the controlof the cell cycle and differentiation; promotes G1/S transition.Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclinsduring interphase at G1 to form a pRB/RB1 kinase and controls theentrance into the cell cycle. Involved in initiation andmaintenance of cell cycle exit during cell differentiation;prevents cell proliferation and regulates negatively celldifferentiation, but is required for the proliferation of specificcell types (e.g. erythroid and hematopoietic cells). Essential forcell proliferation within the dentate gyrus of the hippocampus andthe subventricular zone of the lateral ventricles. Required duringthymocyte development. Promotes the production of newborn neurons,probably by modulating G1 length. Promotes, at least inastrocytes, changes in patterns of gene expression, changes in theactin cytoskeleton including loss of stress fibers, and enhancedmotility during cell differentiation. Prevents myeloiddifferentiation by interfering with RUNX1 and reducing itstranscription transactivation activity, but promotes proliferationof normal myeloid progenitors. Delays senescence. Promotes theproliferation of beta-cells in pancreatic islets of Langerhans.May play a role in the centrosome organization during the cellcycle phases (PubMed:23918663).|
|Tissue Specificity||CDK6: Expressed ubiquitously. Accumulates insquamous cell carcinomas, proliferating hematopoietic progenitorcells, beta-cells of pancreatic islets of Langerhans, andneuroblastomas. Reduced levels in differentiating cells.|
|Sequence Similarities||Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.|
|Subcellular Localization||CDK6: Cytoplasm. Nucleus. Cell projection, ruffle.Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
|Alternative Names||Cyclin-dependent kinase 6 ;Protein Cdk6 ;Cdk6 ;rCG_41083 ;|
|Research Areas|||cell biology|cell cycle|kinases/phosphatases|cdks| epigenetics and nuclear signaling| cancer||