|Reactivity||Human, Mouse, Rat|
|Ig Isotype||Mouse IgG1|
|Product Name||Anti-CDK6 Antibody (Monoclonal, DCS-90)|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Mouse IgG monoclonal antibody for CDK6, cyclin-dependent kinase 6 (CDK6) detection. Tested with WB, ICC in Human;mouse;rat. No cross reactivity with other proteins.|
|Cite This Product||Anti-CDK6 Antibody (Monoclonal, DCS-90) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1021)|
|Immunogen||Recombinant human Cdk6 protein.|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Western blot, 0.5-1μg/ml, Human, mouse, rat
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.
Protein Target Info (Source: Uniprot.org)
|Protein Name||cyclin-dependent kinase 6|
|Tissue Specificity||CDK6: Expressed ubiquitously. Accumulates insquamous cell carcinomas, proliferating hematopoietic progenitorcells, beta-cells of pancreatic islets of Langerhans, andneuroblastomas. Reduced levels in differentiating cells.|
|Alternative Names||Cyclin-dependent kinase 6 ;Protein Cdk6 ;Cdk6 ;rCG_41083 ;|
|Subcellular Localization||CDK6: Cytoplasm. Nucleus. Cell projection, ruffle.Cytoplasm, cytoskeleton, microtubule organizing center, centrosome|Cdk6: Cytoplasm|
|Molecular Weight||36952 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||CDK6: Serine/threonine-protein kinase involved in the controlof the cell cycle and differentiation; promotes G1/S transition.Phosphorylates pRB/RB1 and NPM1. Interacts with D-type G1 cyclinsduring interphase at G1 to form a pRB/RB1 kinase and controls theentrance into the cell cycle. Involved in initiation andmaintenance of cell cycle exit during cell differentiation;prevents cell proliferation and regulates negatively celldifferentiation, but is required for the proliferation of specificcell types (e.g. erythroid and hematopoietic cells). Essential forcell proliferation within the dentate gyrus of the hippocampus andthe subventricular zone of the lateral ventricles. Required duringthymocyte development. Promotes the production of newborn neurons,probably by modulating G1 length. Promotes, at least inastrocytes, changes in patterns of gene expression, changes in theactin cytoskeleton including loss of stress fibers, and enhancedmotility during cell differentiation. Prevents myeloiddifferentiation by interfering with RUNX1 and reducing itstranscription transactivation activity, but promotes proliferationof normal myeloid progenitors. Delays senescence. Promotes theproliferation of beta-cells in pancreatic islets of Langerhans.May play a role in the centrosome organization during the cellcycle phases (PubMed:23918663).|
|Background||The cyclin-dependent protein kinases(CDKs) regulate major cell cycle transitions in eukaryotic cells. Cyclin-dependent kinase-6(CDK6) is the earliest inducible member of the CDK family in human T lymphocytes, involved in growth factor stimulation and cell cycle progression. Germline mutations in CDK6 do not make a significant contribution to melanoma predisposition. CDK6 gene is mapped at 7q21-q22.|
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