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Product Info Summary
| SKU: | MA1021 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | ICC, WB |
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Product info
Product Name
Anti-CDK6 Antibody (Monoclonal, DCS-90)
SKU/Catalog Number
MA1021
BM1616 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CDK6 Antibody (Monoclonal, DCS-90) catalog # MA1021. Tested in ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CDK6 Antibody (Monoclonal, DCS-90) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1021)
Host
Mouse
Contents
Mouse IgG in stabilizing components, 1.2% sodium acetate and 0.01mg NaN3.
Clonality
Monoclonal
Clone Number
DCS-90
Isotype
Mouse IgG1
Immunogen
Recombinant human Cdk6 protein.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
MA1021 is reactive to CDK6 in Human, Mouse, Rat
Observed Molecular Weight
37 kDa
Background of CDK6
The cyclin-dependent protein kinases (CDKs) regulate major cell cycle transitions in eukaryotic cells. Cyclin-dependent kinase-6 (CDK6) is the earliest inducible member of the CDK family in human T lymphocytes, involved in growth factor stimulation and cell cycle progression. Germline mutations in CDK6 do not make a significant contribution to melanoma predisposition. CDK6 gene is mapped at 7q21-q22.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
MA1021 is guaranteed for ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Western blot, 0.5-1μg/ml, Human, mouse, rat
Positive Control
WB: human Jurkat whole cell
Validation Images & Assay Conditions
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Western blot analysis of CDK6 using anti-CDK6 antibody (MA1021).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CDK6 antigen affinity purified monoclonal antibody (Catalog # MA1021) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CDK6 at approximately 37 kDa. The expected band size for CDK6 is at 37 kDa.
Specific Publications For Anti-CDK6 Antibody (Monoclonal, DCS-90) (MA1021)
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Customer Q&As
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15 Customer Q&As for Anti-CDK6 Antibody (Monoclonal, DCS-90)
Question
Here is the WB image, lot number and protocol we used for leukemic t-cell using anti-CDK6 antibody (Monoclonal, DCS-90) MA1021. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-05-07
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-05-07
Question
Does MA1021 anti-CDK6 antibody (Monoclonal, DCS-90) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
You can see on the product datasheet, MA1021 anti-CDK6 antibody (Monoclonal, DCS-90) as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-23
Question
We have seen staining in mouse brain. Do you have any suggestions? Is anti-CDK6 antibody (Monoclonal, DCS-90) supposed to stain brain positively?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
According to literature brain does express CDK6. According to Uniprot.org, CDK6 is expressed in adrenal tissue, tongue, brain, leukemic t-cell, among other tissues. Regarding which tissues have CDK6 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-03-02
Question
Is a blocking peptide available for product anti-CDK6 antibody (Monoclonal, DCS-90) (MA1021)?
Verified Customer
Verified customer
Asked: 2020-02-06
Answer
We do provide the blocking peptide for product anti-CDK6 antibody (Monoclonal, DCS-90) (MA1021). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-02-06
Question
I am looking for to test anti-CDK6 antibody (Monoclonal, DCS-90) MA1021 on human leukemic t-cell for research purposes, then I may be interested in using anti-CDK6 antibody (Monoclonal, DCS-90) MA1021 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
The products we sell, including anti-CDK6 antibody (Monoclonal, DCS-90) MA1021, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-12-05
Question
Is this MA1021 anti-CDK6 antibody (Monoclonal, DCS-90) reactive to the isotypes of CDK6?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
The immunogen of MA1021 anti-CDK6 antibody (Monoclonal, DCS-90) is Recombinant human Cdk6 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-26
Question
We purchased anti-CDK6 antibody (Monoclonal, DCS-90) for ICC on tongue in a previous project. I am using rat, and We intend to use the antibody for WB next. you antibody examining tongue as well as brain in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
I viewed the website and datasheets of our anti-CDK6 antibody (Monoclonal, DCS-90) and I see that MA1021 has been validated on rat in both ICC and WB. Thus MA1021 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-09
Question
My question regarding product MA1021, anti-CDK6 antibody (Monoclonal, DCS-90). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Edwards
Verified customer
Asked: 2019-01-29
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1021 anti-CDK6 antibody (Monoclonal, DCS-90), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-01-29
Question
My colleagues were satisfied with the WB result of your anti-CDK6 antibody (Monoclonal, DCS-90). However we have observed positive staining in brain ruffle. using this antibody. Is that expected? Could you tell me where is CDK6 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-06-15
Answer
According to literature, brain does express CDK6. Generally CDK6 expresses in cytoplasm. nucleus. cell projection, ruffle. Regarding which tissues have CDK6 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Tongue, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-06-15
Question
We are currently using anti-CDK6 antibody (Monoclonal, DCS-90) MA1021 for mouse tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-31
Answer
The anti-CDK6 antibody (Monoclonal, DCS-90) (MA1021) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-31
Question
Do you have a BSA free version of anti-CDK6 antibody (Monoclonal, DCS-90) MA1021 available?
Verified Customer
Verified customer
Asked: 2017-08-24
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CDK6 antibody (Monoclonal, DCS-90) MA1021 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-08-24
Question
I see that the anti-CDK6 antibody (Monoclonal, DCS-90) MA1021 works with ICC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-05-23
Answer
You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-05-23
Question
I was wanting to use your anti-CDK6 antibody (Monoclonal, DCS-90) for ICC for human leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukemic t-cell identification?
P. Jones
Verified customer
Asked: 2015-05-06
Answer
As indicated on the product datasheet, MA1021 anti-CDK6 antibody (Monoclonal, DCS-90) has been tested for ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-05-06
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-CDK6 antibody (Monoclonal, DCS-90) MA1021. Let me know if you need anything else.
K. Miller
Verified customer
Asked: 2014-03-27
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-03-27
Question
Would anti-CDK6 antibody (Monoclonal, DCS-90) MA1021 work for ICC with leukemic t-cell?
K. Mitchell
Verified customer
Asked: 2013-12-17
Answer
According to the expression profile of leukemic t-cell, CDK6 is highly expressed in leukemic t-cell. So, it is likely that anti-CDK6 antibody (Monoclonal, DCS-90) MA1021 will work for ICC with leukemic t-cell.
Boster Scientific Support
Answered: 2013-12-17
