Anti-CDK6 Antibody (Monoclonal, DCS-90)

Boster Bio Anti-CDK6 Antibody (Monoclonal, DCS-90) catalog # MA1021. Tested in ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 4 publication(s).

Product Info Summary

SKU: MA1021
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Mouse
Application: ICC, WB

Product Name

Anti-CDK6 Antibody (Monoclonal, DCS-90)

See all CDK6 products

SKU/Catalog Number

MA1021

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CDK6 Antibody (Monoclonal, DCS-90) catalog # MA1021. Tested in ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CDK6 Antibody (Monoclonal, DCS-90) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1021)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

DCS-90

Isotype

Mouse IgG1

Immunogen

Recombinant human Cdk6 protein.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

MA1021 is reactive to CDK6 in Human, Mouse, Rat

Applications

MA1021 is guaranteed for ICC, WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunocytochemistry , 1μg/ml, Human, mouse, rat, -
Western blot, 0.5-1μg/ml, Human, mouse, rat

Validation Images & Assay Conditions

Gene/Protein Information For CDK6 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q00534

Gene ID

1021

Gene Name

CDK6

Full Name

Cyclin-dependent kinase 6

Weight

36.938kDa

Superfamily

protein kinase superfamily

Alternative Names

Cell division protein kinase 6; cyclin-dependent kinase 6; EC 2.7.11; EC 2.7.11.22; PLSTIREMGC59692; Serine/threonine-protein kinase PLSTIRE CDK6 MCPH12, PLSTIRE cyclin dependent kinase 6 cyclin-dependent kinase 6|cell division protein kinase 6|serine/threonine-protein kinase PLSTIRE

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CDK6, check out the CDK6 Infographic

CDK6 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CDK6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

MA1021 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Sun D,Zhao T,Long K,Wu M,Zhang Z.Triclosan down-regulates fatty acid synthase through microRNAs in HepG2 cells.Eur J Pharmacol .2021 Jun 15:174261.doi:10.1016/j.ejphar.2021.174261.Epub ahead of print.PMID:34144025.
Species: Human
MA1021 usage in article: APP:WB, SAMPLE:HEPG2 CELL, DILUTION:1:400

Long noncoding RNA NEAT1 promotes laryngeal squamous cell cancer through regulating miR-107/CDK6 pathway

Ren J, Huang Q, Xu Y, Yang M, Yang J, Hu K. Anticancer Drugs. 2015 Jul;26(6):599-611. Doi: 10.1097/Cad.0000000000000224. Isoflavone Lupiwighteone Induces Cytotoxic, Apoptotic, And Antiangiogenic Activities In Du-145 Prostate Cancer Cells.

Zhou R, Lu Z, Liu K, Guo J, Liu J, Zhou Y, Yang J, Mi M, Xu H. Curr Cancer Drug Targets. 2015;14(9):860-71. Platycodin D Induces Tumor Growth Arrest By Activating Foxo3A Expression In Prostate Cancer In Vitro And In Vivo.

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15 Customer Q&As for Anti-CDK6 Antibody (Monoclonal, DCS-90)

Question

Here is the WB image, lot number and protocol we used for leukemic t-cell using anti-CDK6 antibody (Monoclonal, DCS-90) MA1021. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-05-07

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-05-07

Question

Does MA1021 anti-CDK6 antibody (Monoclonal, DCS-90) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

You can see on the product datasheet, MA1021 anti-CDK6 antibody (Monoclonal, DCS-90) as been validated on ICC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-04-23

Question

We have seen staining in mouse brain. Do you have any suggestions? Is anti-CDK6 antibody (Monoclonal, DCS-90) supposed to stain brain positively?

Verified Customer

Verified customer

Asked: 2020-03-02

Answer

According to literature brain does express CDK6. According to Uniprot.org, CDK6 is expressed in adrenal tissue, tongue, brain, leukemic t-cell, among other tissues. Regarding which tissues have CDK6 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Tongue, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2020-03-02

Question

Is a blocking peptide available for product anti-CDK6 antibody (Monoclonal, DCS-90) (MA1021)?

Verified Customer

Verified customer

Asked: 2020-02-06

Answer

We do provide the blocking peptide for product anti-CDK6 antibody (Monoclonal, DCS-90) (MA1021). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-02-06

Question

I am looking for to test anti-CDK6 antibody (Monoclonal, DCS-90) MA1021 on human leukemic t-cell for research purposes, then I may be interested in using anti-CDK6 antibody (Monoclonal, DCS-90) MA1021 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-05

Answer

The products we sell, including anti-CDK6 antibody (Monoclonal, DCS-90) MA1021, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-05

Question

Is this MA1021 anti-CDK6 antibody (Monoclonal, DCS-90) reactive to the isotypes of CDK6?

Verified Customer

Verified customer

Asked: 2019-07-26

Answer

The immunogen of MA1021 anti-CDK6 antibody (Monoclonal, DCS-90) is Recombinant human Cdk6 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-07-26

Question

We purchased anti-CDK6 antibody (Monoclonal, DCS-90) for ICC on tongue in a previous project. I am using rat, and We intend to use the antibody for WB next. you antibody examining tongue as well as brain in our next experiment. Could give a recommendation on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-07-09

Answer

I viewed the website and datasheets of our anti-CDK6 antibody (Monoclonal, DCS-90) and I see that MA1021 has been validated on rat in both ICC and WB. Thus MA1021 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-07-09

Question

My question regarding product MA1021, anti-CDK6 antibody (Monoclonal, DCS-90). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

T. Edwards

Verified customer

Asked: 2019-01-29

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free MA1021 anti-CDK6 antibody (Monoclonal, DCS-90), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-01-29

Question

My colleagues were satisfied with the WB result of your anti-CDK6 antibody (Monoclonal, DCS-90). However we have observed positive staining in brain ruffle. using this antibody. Is that expected? Could you tell me where is CDK6 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-06-15

Answer

According to literature, brain does express CDK6. Generally CDK6 expresses in cytoplasm. nucleus. cell projection, ruffle. Regarding which tissues have CDK6 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Tongue, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2018-06-15

Question

We are currently using anti-CDK6 antibody (Monoclonal, DCS-90) MA1021 for mouse tissue, and we are happy with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2018-01-31

Answer

The anti-CDK6 antibody (Monoclonal, DCS-90) (MA1021) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-01-31

Question

Do you have a BSA free version of anti-CDK6 antibody (Monoclonal, DCS-90) MA1021 available?

Verified Customer

Verified customer

Asked: 2017-08-24

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CDK6 antibody (Monoclonal, DCS-90) MA1021 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-08-24

Question

I see that the anti-CDK6 antibody (Monoclonal, DCS-90) MA1021 works with ICC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2017-05-23

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2017-05-23

Question

I was wanting to use your anti-CDK6 antibody (Monoclonal, DCS-90) for ICC for human leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukemic t-cell identification?

P. Jones

Verified customer

Asked: 2015-05-06

Answer

As indicated on the product datasheet, MA1021 anti-CDK6 antibody (Monoclonal, DCS-90) has been tested for ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2015-05-06

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-CDK6 antibody (Monoclonal, DCS-90) MA1021. Let me know if you need anything else.

K. Miller

Verified customer

Asked: 2014-03-27

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2014-03-27

Question

Would anti-CDK6 antibody (Monoclonal, DCS-90) MA1021 work for ICC with leukemic t-cell?

K. Mitchell

Verified customer

Asked: 2013-12-17

Answer

According to the expression profile of leukemic t-cell, CDK6 is highly expressed in leukemic t-cell. So, it is likely that anti-CDK6 antibody (Monoclonal, DCS-90) MA1021 will work for ICC with leukemic t-cell.

Boster Scientific Support

Answered: 2013-12-17

Size

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MA1021
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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