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Product Info Summary
| SKU: | M00356-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CEA (CD66e) CEACAM5 Rabbit Monoclonal Antibody
View all CEACAM5/CD66e Antibodies
SKU/Catalog Number
M00356-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CEA (CD66e) CEACAM5 Rabbit Monoclonal Antibody catalog # M00356-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CEA (CD66e) CEACAM5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00356-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AAOA-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CEA (CD66e)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00356-1 is reactive to CEACAM5 in Human
Applications
M00356-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
23 kDa
Calculated molecular weight
76.795kDa
Background of CEACAM5/CD66e
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Validation Images & Assay Conditions
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Western blot analysis of CEA(CD66e) expression in Human colon cancer lysate.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using CEA(CD66e) Antibody.
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Immunofluorescent analysis using the Antibody at 1:150 dilution.
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Immunofluorescent analysis of MCF-7 cells, using CEA(CD66e) Antibody.
Protein Target Info & Infographic
Gene/Protein Information For CEACAM5 (Source: Uniprot.org, NCBI)
Gene Name
CEACAM5
Full Name
Carcinoembryonic antigen-related cell adhesion molecule 5
Weight
76.795kDa
Superfamily
immunoglobulin superfamily
Alternative Names
Carcinoembryonic antigen; carcinoembryonic antigen-related cell adhesion molecule 5; CD66e antigen; CD66e; CEA; CEACAM5; CEACAM-5; CEACD66e; DKFZp781M2392; Meconium antigen 100 CEACAM5 CD66e, CEA CEA cell adhesion molecule 5 carcinoembryonic antigen-related cell adhesion molecule 5|carcinoembryonic antigen related cell adhesion molecule 5|meconium antigen 100
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CEACAM5, check out the CEACAM5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CEACAM5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CEA (CD66e) CEACAM5 Rabbit Monoclonal Antibody (M00356-1)
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No publications found for M00356-1
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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15 Customer Q&As for Anti-CEA (CD66e) CEACAM5 Rabbit Monoclonal Antibody
Question
We have seen staining in human colonic mucosa. Are there any suggestions? Is anti-CEA (CD66e) Rabbit Monoclonal antibody supposed to stain colonic mucosa positively?
Verified Customer
Verified customer
Asked: 2020-02-20
Answer
According to literature colonic mucosa does express CEACAM5. According to Uniprot.org, CEACAM5 is expressed in colonic mucosa, saliva, liver, among other tissues. Regarding which tissues have CEACAM5 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Saliva, Pubmed ID: 16740002
Boster Scientific Support
Answered: 2020-02-20
Question
We are currently using anti-CEA (CD66e) Rabbit Monoclonal antibody M00356-1 for human tissue, and we are content with the ICC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
The anti-CEA (CD66e) Rabbit Monoclonal antibody (M00356-1) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-20
Question
Please see the WB image, lot number and protocol we used for colonic mucosa using anti-CEA (CD66e) Rabbit Monoclonal antibody M00356-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-04
Question
Is there a BSA free version of anti-CEA (CD66e) Rabbit Monoclonal antibody M00356-1 available?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CEA (CD66e) Rabbit Monoclonal antibody M00356-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-20
Question
I was wanting to use your anti-CEA (CD66e) Rabbit Monoclonal antibody for IHC for human colonic mucosa on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human colonic mucosa identification?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
As indicated on the product datasheet, M00356-1 anti-CEA (CD66e) Rabbit Monoclonal antibody has been tested for IF, IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human colonic mucosa in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-26
Question
Is a blocking peptide available for product anti-CEA (CD66e) Rabbit Monoclonal antibody (M00356-1)?
Verified Customer
Verified customer
Asked: 2019-06-14
Answer
We do provide the blocking peptide for product anti-CEA (CD66e) Rabbit Monoclonal antibody (M00356-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-14
Question
My question regarding product M00356-1, anti-CEA (CD66e) Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
L. Roberts
Verified customer
Asked: 2019-05-14
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00356-1 anti-CEA (CD66e) Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-14
Question
My team were satisfied with the WB result of your anti-CEA (CD66e) Rabbit Monoclonal antibody. However we have observed positive staining in saliva cell membrane using this antibody. Is that expected? Could you tell me where is CEACAM5 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-01-24
Answer
Based on literature, saliva does express CEACAM5. Generally CEACAM5 expresses in cell membrane. Regarding which tissues have CEACAM5 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Saliva, Pubmed ID: 16740002
Boster Scientific Support
Answered: 2019-01-24
Question
Would anti-CEA (CD66e) Rabbit Monoclonal antibody M00356-1 work for IHC with colonic mucosa?
Verified Customer
Verified customer
Asked: 2018-01-09
Answer
According to the expression profile of colonic mucosa, CEACAM5 is highly expressed in colonic mucosa. So, it is likely that anti-CEA (CD66e) Rabbit Monoclonal antibody M00356-1 will work for IHC with colonic mucosa.
Boster Scientific Support
Answered: 2018-01-09
Question
We have tried in the past anti-CEA (CD66e) Rabbit Monoclonal antibody for ICC on saliva a few years ago. I am using human, and We want to use the antibody for IHC next. My question regards examining saliva as well as colonic mucosa in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
A. Zhao
Verified customer
Asked: 2017-12-21
Answer
I have checked the website and datasheets of our anti-CEA (CD66e) Rabbit Monoclonal antibody and it appears that M00356-1 has been validated on human in both ICC and IHC. Thus M00356-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-12-21
Question
Is this M00356-1 anti-CEA (CD66e) Rabbit Monoclonal antibody reactive to the isotypes of CEACAM5?
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
The immunogen of M00356-1 anti-CEA (CD66e) Rabbit Monoclonal antibody is A synthesized peptide derived from human CEA(CD66e). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-10-02
Question
I see that the anti-CEA (CD66e) Rabbit Monoclonal antibody M00356-1 works with IHC, what is the protocol used to produce the result images on the product page?
M. Li
Verified customer
Asked: 2017-09-04
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-09-04
Question
I would like to test anti-CEA (CD66e) Rabbit Monoclonal antibody M00356-1 on human colonic mucosa for research purposes, then I may be interested in using anti-CEA (CD66e) Rabbit Monoclonal antibody M00356-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-07-25
Answer
The products we sell, including anti-CEA (CD66e) Rabbit Monoclonal antibody M00356-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-07-25
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colonic mucosa using anti-CEA (CD66e) Rabbit Monoclonal antibody M00356-1. Let me know if you need anything else.
D. Baker
Verified customer
Asked: 2013-09-25
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-09-25
Question
Would M00356-1 anti-CEA (CD66e) Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Krishna
Verified customer
Asked: 2013-07-10
Answer
You can see on the product datasheet, M00356-1 anti-CEA (CD66e) Rabbit Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-07-10
