Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1)

Product Name

Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1)

View all Cyclin A2 Antibodies

SKU/Catalog Number

MA1032

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1) catalog # MA1032. Tested in ICC, WB applications. This antibody reacts with Bovine, Human, Mouse.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cyclin A Ccna2 Antibody (Monoclonal, CY-A1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1032)

Host

Mouse

Contents

Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.

Clonality

Monoclonal

Clone Number

CY-A1

Isotype

Mouse IgG2a

Immunogen

Recombinant bovine cyclin A.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

MA1032 is reactive to CCNA2 in Bovine, Human, Mouse

Applications

MA1032 is guaranteed for ICC, WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

48.551kDa

Background of Cyclin A2

The mammalian A-type cyclin family consists of 2 members, cyclin A1 (CCNA1) and cyclin A2 (CCNA2). Human cyclin A gene is assigned to 4q26-q27. Cyclin A protein is synthesized and localized into the nucleus at the onset of S phase in nontransformed mammalian fibroblasts.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 1ml of PBS buffer will yield a concentration of 100ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunocytochemistry , 1μg/ml, Human, bovine , mouse, -
Western blot, 0.5-1μg/ml, Human, bovine , mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Cyclin A using anti-Cyclin A antibody (MA1032).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cyclin A antigen affinity purified monoclonal antibody (Catalog # MA1032) at 1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cyclin A at approximately 55 kDa. The expected band size for Cyclin A is at 49 kDa.

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Figure 2. ICC analysis of Cyclin A using anti-Cyclin A antibody (MA1032).
Cyclin A was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml mouse anti-Cyclin A Antibody (MA1032) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

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Figure 3. ICC analysis of Cyclin A using anti-Cyclin A antibody (MA1032).
Cyclin A was detected in an immunocytochemical section of SMMC-7721 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 μg/ml mouse anti-Cyclin A Antibody (MA1032) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Gene/Protein Information For CCNA2 (Source: Uniprot.org, NCBI)

Alternative Names

CCN1CCNAcyclin-A; CCNA; CCNA2; Cyclin A2; Cyclin-A; cyclin-A2 CCNA2 CCN1, CCNA cyclin A2 cyclin-A2|cyclin-A

For more info on CCNA2, check out the CCNA2 Infographic

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In this infographic, you will see the following information for CCNA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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