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Product Info Summary
| SKU: | A09728 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Cysteine protease ATG4C ATG4C Antibody
SKU/Catalog Number
A09728
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Cysteine protease ATG4C ATG4C Antibody (Catalog # A09728). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ATG4C antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Cysteine protease ATG4C ATG4C Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09728)
Host
Rabbit
Contents
ATG4C antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
ATG4C antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ATG4C. The immunogen is located within the last 50 amino acids of ATG4C.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
ATG4C antibody is human, mouse and rat reactive. ATG4C is predicted to not cross-react with other ATG4 proteins.
Reactive Species
A09728 is reactive to ATG4C in Human, Mouse, Rat
Applications
A09728 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
52.497kDa
Background of ATG4C
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). ATG4C, also known as autophagin-3, is one of four mammalian orthologs of the yeast ATG4 protein; all four are cysteine proteases (3). Recent studies have shown that ATG4C and Beclin-1 are regulated by the microRNA miR-376b; overexpression of miR-376b led to decreased mRNA and protein levels, thereby blocking starvation and TOR inhibition-related autophagy (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ATG4C antibody can be used for detection of ATG4C by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ATG4C in A549 cell lysate with ATG4C antibody at 1 μg/ml.
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Immunohistochemistry of ATG4C in human lung tissue with ATG4C antibody at 5 μg/ml.
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Immunofluorescence of ATG4C in human lung tissue with ATG4C antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For ATG4C (Source: Uniprot.org, NCBI)
Gene Name
ATG4C
Full Name
Cysteine protease ATG4C
Weight
52.497kDa
Superfamily
peptidase C54 family
Alternative Names
APG4 autophagy 4 homolog C (S. cerevisiae); APG4 autophagy 4 homolog C; APG4C; ATG4 autophagy related 4 homolog C (S. cerevisiae); AUT (S. cerevisiae)-like 1, cysteine endopeptidase; AUT-like 1, cysteineendopeptidase (S. cerevisiae); AUTL1; AUTL3APG4-C; AUT-like 1, cysteine endopeptidase; AUT-like 3 cysteine endopeptidase; autophagin-3; Autophagy-related cysteine endopeptidase 3; Autophagy-related protein 4 homolog C; cysteine protease ATG4C; EC 3.4.22; EC 3.4.22.-; FLJ14867 ATG4C APG4-C, APG4C, AUTL1, AUTL3 autophagy related 4C cysteine peptidase cysteine protease ATG4C|APG4 autophagy 4 homolog C|ATG4 autophagy related 4 homolog C|AUT-like 1, cysteine endopeptidase|AUT-like 3 cysteine endopeptidase|autophagin-3|autophagy-related cysteine endopeptidase 3|autophagy-related protein 4 homolog C|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATG4C, check out the ATG4C Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATG4C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cysteine protease ATG4C ATG4C Antibody (A09728)
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No publications found for A09728
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