Anti-DKK1 Rabbit Monoclonal Antibody

Product Name

Anti-DKK1 Rabbit Monoclonal Antibody

View all Dkk-1 Antibodies

SKU/Catalog Number

M00632

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-DKK1 Rabbit Monoclonal Antibody catalog # M00632. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DKK1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00632)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

FHG-4

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human DKK1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00632 is reactive to Dkk1 in Human, Rat

Applications

M00632 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

26 kDa

Calculated molecular weight

29.298kDa

Background of Dkk-1

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.

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Immunohistochemical analysis of paraffin-embedded human placenta, using DKK1 Antibody.

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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.

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Western blot analysis of DKK1 expression in HeLa cell lysate.

Gene/Protein Information For Dkk1 (Source: Uniprot.org, NCBI)

Alternative Names

dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; Dkk1; Dkk-1; hDkk-1; SKdickkopf-1 like Dkk1|mdkk-1|dickkopf WNT signaling pathway inhibitor 1|dickkopf-related protein 1|dickkopf homolog 1|dickkopf-1|dickkopf-like protein 1|dkk-1

For more info on Dkk1, check out the Dkk1 Infographic

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In this infographic, you will see the following information for Dkk1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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