Product Info Summary
SKU: | PA2004 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-DR3/TNFRSF25 Antibody
View all DR3/TNFRSF25 Antibodies
SKU/Catalog Number
PA2004
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-DR3/TNFRSF25 Antibody catalog # PA2004. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DR3/TNFRSF25 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2004)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human DR3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2004 is reactive to TNFRSF25 in Human
Applications
PA2004 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
59 kDa
Calculated molecular weight
45.385kDa
Background of DR3/TNFRSF25
TNFRSF25 (Tumor Necrosis Factor Receptor Superfamily Member 25), also known as LARD, APO3, DR3 or TNFR25, is a protein that in humans is encoded by the TNFRSF25 gene. Members of the mammalian tumor necrosis factor receptor (TNFR) family are cell-surface proteins that interact with a corresponding TNF-related ligand family. By fluorescence in situ hybridization, Marsters et al. (1996) mapped the Apo3 gene to 1p36.3. Marsters et al. (1996) showed that ectopic expression of Apo3 in mammalian cells triggered apoptosis and activated the transcription factor NF-kappa-B. They suggested that, like TNFR1, Apo3 may regulate distinct signaling pathways in different cellular contexts.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Anti-DR3 antibody, PA2004, Western blotting
All lanes: Anti DR3 (PA2004) at 0.5ug/ml
WB: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 59KD
Observed bind size: 59KD
Protein Target Info & Infographic
Gene/Protein Information For TNFRSF25 (Source: Uniprot.org, NCBI)
Gene Name
TNFRSF25
Full Name
Tumor necrosis factor receptor superfamily member 25
Weight
45.385kDa
Alternative Names
APO3; Apo-3; Apoptosis-inducing receptor AIR; Apoptosis-mediating receptor DR3; Apoptosis-mediating receptor TRAMP; Death receptor 3; DR3; DR3member 12 (translocatingchain-association membrane protein); LARD; Lymphocyte-associated receptor of death; Protein WSL; Protein WSL-1; TNFRSF25; TRAMP; tumor necrosis factor receptor superfamily, member 25; WSL; WSL1; WSL-1 TNFRSF25 APO-3, DDR3, DR3, GEF720, LARD, PLEKHG5, TNFRSF12, TR3, TRAMP, WSL-1, WSL-LR TNF receptor superfamily member 25 tumor necrosis factor receptor superfamily member 25|Guanine nucleotide exchange factor 720|PH domain-containing family G member 5|Pleckstrin homology domain-containing family G member 5|apoptosis inducing receptor|apoptosis-inducing receptor AIR|apoptosis-mediating receptor DR3|apoptosis-mediating receptor TRAMP|death domain receptor 3 soluble form|death receptor beta|lymphocyte-associated receptor of death|protein WSL-1|tumor necrosis factor receptor superfamily, member 12 (translocating chain-association membrane protein)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNFRSF25, check out the TNFRSF25 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNFRSF25: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-DR3/TNFRSF25 Antibody (PA2004)
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No publications found for PA2004
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-DR3/TNFRSF25 Antibody
Question
I was wanting to use your anti-DR3/TNFRSF25 antibody for WB for human heart on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human heart identification?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
It shows on the product datasheet, PA2004 anti-DR3/TNFRSF25 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human heart in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-06
Question
We are currently using anti-DR3/TNFRSF25 antibody PA2004 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
The anti-DR3/TNFRSF25 antibody (PA2004) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-10
Question
Here is the WB image, lot number and protocol we used for heart using anti-DR3/TNFRSF25 antibody PA2004. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-05
Question
Is this PA2004 anti-DR3/TNFRSF25 antibody reactive to the isotypes of TNFRSF25?
D. Roberts
Verified customer
Asked: 2014-02-26
Answer
The immunogen of PA2004 anti-DR3/TNFRSF25 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human DR3(73-94aa CPQDTFLAWENHHNSECARCQA). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-02-26
Question
Would PA2004 anti-DR3/TNFRSF25 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
L. Krishna
Verified customer
Asked: 2013-10-30
Answer
You can see on the product datasheet, PA2004 anti-DR3/TNFRSF25 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-10-30