Click image to see more details
Product Info Summary
| SKU: | M00063-3 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-E-Cadherin 1 (CDH1) Rabbit Monoclonal Antibody, Clone#RM244
SKU/Catalog Number
M00063-3
Size
100ul
Form
Liquid
Description
Boster Bio Anti-E-Cadherin 1 (CDH1) Rabbit Monoclonal Antibody, Clone#RM244 (Catalog # M00063-3). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-E-Cadherin 1 (CDH1) Rabbit Monoclonal Antibody, Clone#RM244 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00063-3)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM244
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to Cadherin-1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human E-cadherin (Cadherin-1).
Reactive Species
M00063-3 is reactive to CDH1 in Human
Applications
M00063-3 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
97.456kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:500-1:1000 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of MCF-7 cell lysates using Anti-E-cadherin RM244 at a 1:1000 dilution, showed a band of E-cadherin (~120 kDa) expressed in MCF-7 cells.
Click image to see more details
Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-E-cadherin RM244 at a 1:1000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CDH1 (Source: Uniprot.org, NCBI)
Gene Name
CDH1
Full Name
Cadherin-1
Weight
97.456kDa
Alternative Names
Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin CDH1 Arc-1, BCDS1, CD324, CDHE, ECAD, LCAM, UVO cadherin 1 cadherin-1|CAM 120/80|E-cadherin 1|cadherin 1, E-cadherin (epithelial)|cadherin 1, type 1, E-cadherin (epithelial)|calcium-dependent adhesion protein, epithelial|cell-CAM 120/80|epididymis secretory sperm binding protein|epithelial cadherin|uvomorulin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDH1, check out the CDH1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDH1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-E-Cadherin 1 (CDH1) Rabbit Monoclonal Antibody, Clone#RM244 (M00063-3)
Hello CJ!
M00063-3 has been cited in 13 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Hsa_circ_0137008 suppresses the malignant phenotype in colorectal cancer by acting as a microRNA-338-5p sponge
Piao HY,Guo S,Wang Y,Zhang J.Exosome-transmitted lncRNA PCGEM1 promotes invasive and metastasis in gastric cancer by maintaining the stability of SNAI1.Clin Transl Oncol.2020 Jun 9.doi:10.1007/s12094-020-02412-9.Epub ahead of print.PMID:32519176.
Species: Human
Yang Z,Zhang J,Lu D,Sun Y, Zhao X,Wang X,Zhou W,He Q,Jiang Z.Hsa_circ_0137008 suppresses the malignant phenotype in colorectal cancer by acting as a microRNA-338-5p sponge. Cancer Cell Int.2020 Mar 4;20:67. doi:10.1186/s1293 5-020-1150-1.PMID:32158357;PMC
Species: Human
M00063-3 usage in article: APP:WB, SAMPLE:SW480 AND HCT116 CELLS, DILUTION:NA
Detection of epithelial to mesenchymal transition in airways of a bleomycin induced pulmonary fibrosis model derived from an %u03B1-smooth muscle actin-Cre %u2026
HIF%u20101%u03B1 binding to AEG%u20101 promoter induced upregulated AEG%u20101 expression associated with metastasis in ovarian cancer
Frizzled2 mediates the migration and invasion of human oral squamous cell carcinoma cells through the regulation of the signal transducer and activator of %u2026
Overexpression of SDF-1 activates the NF-%u03BAB pathway to induce epithelial to mesenchymal transition and cancer stem cell-like phenotypes of breast cancer cells
RhoC is essential in TGF-?1 induced epithelial-mesenchymal transition in cervical cancer cells.
Connective tissue growth factor is overexpressed in human hepatocellular carcinoma and promotes cell invasion and growth
Overexpression of Livin promotes migration and invasion of colorectal cancer cells by induction of epithelial-mesenchymal transition via NF-?B activation
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-E-Cadherin 1 (CDH1) Rabbit Monoclonal Antibody, Clone#RM244
Question
Does anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 M00063-3 work for WB with pancreas?
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
According to the expression profile of pancreas, CDH1 is highly expressed in pancreas. So, it is likely that anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 M00063-3 will work for WB with pancreas.
Boster Scientific Support
Answered: 2019-11-29
Question
I have attached the WB image, lot number and protocol we used for pancreas using anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 M00063-3. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-01
Question
Is this M00063-3 anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 reactive to the isotypes of CDH1?
Verified Customer
Verified customer
Asked: 2019-09-18
Answer
The immunogen of M00063-3 anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 is A peptide corresponding to Cadherin-1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-18
Question
We ordered your anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-07-02
Answer
I took a look at the website and datasheets of our anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2019-07-02
Question
Is a blocking peptide available for product anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 (M00063-3)?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
We do provide the blocking peptide for product anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 (M00063-3). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-05
Question
I have a question about product M00063-3, anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00063-3 anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-24
Question
Do you have a BSA free version of anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 M00063-3 available?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 M00063-3 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-05-15
Question
My team were satisfied with the WB result of your anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2018-10-10
Answer
Based on literature, brain does express CDH1. Generally CDH1 expresses in cell junction, adherens junction. Regarding which tissues have CDH1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14595118
Epidermal carcinoma, Pubmed ID: 10597309
Hippocampus, and Tongue, Pubmed ID: 14702039
Liver, Pubmed ID: 3263290, 8185635, 24275569, 8033105, 11953314
Pancreas, Pubmed ID: 8459805
Placenta, Pubmed ID: 7543680, 7601454
Boster Scientific Support
Answered: 2018-10-10
Question
We have observed staining in human jejunal mucosa. Any tips? Is anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 supposed to stain jejunal mucosa positively?
C. Thomas
Verified customer
Asked: 2018-08-30
Answer
From literature jejunal mucosa does express CDH1. From Uniprot.org, CDH1 is expressed in jejunal mucosa, pancreas, liver, epidermal carcinoma, hippocampus tongue, placenta, brain, among other tissues. Regarding which tissues have CDH1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 14595118
Epidermal carcinoma, Pubmed ID: 10597309
Hippocampus, and Tongue, Pubmed ID: 14702039
Liver, Pubmed ID: 3263290, 8185635, 24275569, 8033105, 11953314
Pancreas, Pubmed ID: 8459805
Placenta, Pubmed ID: 7543680, 7601454
Boster Scientific Support
Answered: 2018-08-30
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreas using anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 M00063-3. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-02-09
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-02-09
Question
We need to test anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 M00063-3 on human pancreas for research purposes, then I may be interested in using anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 M00063-3 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-10-11
Answer
The products we sell, including anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 M00063-3, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-11
Question
I see that the anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 M00063-3 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-08-09
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-08-09
Question
I was wanting to use your anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 for WB for human pancreas on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human pancreas identification?
M. Anderson
Verified customer
Asked: 2017-06-26
Answer
As indicated on the product datasheet, M00063-3 anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human pancreas in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-06-26
Question
My lab would like using your anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 for immunoregulatory interactions between a lymphoid and a non-lymphoid cell studies. Has this antibody been tested with western blotting on breast cancer tissue? We would like to see some validation images before ordering.
J. Lewis
Verified customer
Asked: 2017-01-12
Answer
We appreciate your inquiry. This M00063-3 anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 is tested on breast cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-01-12
Question
We are currently using anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 M00063-3 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?
V. Johnson
Verified customer
Asked: 2016-03-03
Answer
The anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 (M00063-3) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-03-03
Question
Will M00063-3 anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
P. Miller
Verified customer
Asked: 2014-08-06
Answer
As indicated on the product datasheet, M00063-3 anti-E-Cadherin (CDH1) Rabbit Monoclonal antibody, Clone#RM244 as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-08-06
