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Product Info Summary
| SKU: | PA1398 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Pro-epidermal growth factor EGF Antibody
SKU/Catalog Number
PA1398
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Pro-epidermal growth factor EGF Antibody catalog # PA1398. Tested in IHC, WB applications. This antibody reacts with Mouse.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Pro-epidermal growth factor EGF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1398)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse EGF.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1398 is reactive to EGF in Mouse
Applications
PA1398 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
40 kDa
Calculated molecular weight
133.994kDa
Background of EGF
Epidermal growth factor (EGF) is a growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation by binding to its receptor EGFR. EGF locus is mapped to 4q21-4qter. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bones. EGF results in cellular proliferation, differentiation, and survival while plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Mouse
Validation Images & Assay Conditions
Click image to see more details
Anti-EGF antibody, PA1398, Western blotting
Lane 1: Recombinant Mouse EGF Protein 10ng
Lane 2: Recombinant Mouse EGF Protein 5ng
Lane 3: Recombinant Mouse EGF Protein 2.5ng
Click image to see more details
Anti-EGF antibody, PA1398, IHC(P)
IHC(P): Mouse Intestine Tissue
Protein Target Info & Infographic
Gene/Protein Information For EGF (Source: Uniprot.org, NCBI)
Gene Name
EGF
Full Name
Pro-epidermal growth factor
Weight
133.994kDa
Alternative Names
beta-urogastrone; EGF; epidermal growth factor (beta-urogastrone); epidermal growth factor; HOMG4; pro-epidermal growth factor; URG; Urogastrone EGF HOMG4, URG epidermal growth factor pro-epidermal growth factor|beta-urogastrone
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EGF, check out the EGF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EGF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Pro-epidermal growth factor EGF Antibody (PA1398)
Hello CJ!
PA1398 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of IL-10 on the expression of HSC growth factors in hepatic fibrosis rat
Ruan R, Jin P, Zhang L, Wang C, Chen C, Ding W, Wen L. Mol Pharm. 2014 Nov 3;11(11):4015-22. Doi: 10.1021/Mp500277G. Epub 2014 Oct 13. Peptide-Chaperone-Directed Transdermal Protein Delivery Requires Energy.
Ge C, Yu M, Petitte Jn, Zhang C. Biol Reprod. 2009 Mar;80(3):528-36. Doi: 10.1095/Biolreprod.108.072728. Epub 2008 Nov 12. Epidermal Growth Factor-Induced Proliferation Of Chicken Primordial Germ Cells: Involvement Of Calcium/Protein Kinase C And ...
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
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Find answers in Q&As, reviews.
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17 Customer Q&As for Anti-Pro-epidermal growth factor EGF Antibody
Question
For PA1398, It seems that the customer is wondering if this antibody could be used for IHC for fresh cells or frozen sections and not paraffin embedded samples? Do you have any information?
Verified Customer
Verified customer
Asked: 2020-05-01
Answer
Our lab technicians failed to get positive staining in IHC for fresh cells and frozen sections with PA1398.
Boster Scientific Support
Answered: 2020-05-01
Question
Is this PA1398 anti-EGF antibody reactive to the isotypes of EGF?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
The immunogen of PA1398 anti-EGF antibody is A synthetic peptide corresponding to a sequence at the C-terminus of mouse EGF(1013-1029aa YSGDRCQTRDLRWWELR). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-03-25
Question
We are currently using anti-EGF antibody PA1398 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse. Is it true that the antibody can work on dog tissues as well?
J. Miller
Verified customer
Asked: 2019-12-17
Answer
The anti-EGF antibody (PA1398) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-17
Question
My question regards using your anti-EGF antibody for branching morphogenesis of an epithelial tube studies. Has this antibody been tested with western blotting on mouse intestine tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
We appreciate your inquiry. This PA1398 anti-EGF antibody is validated on mouse intestine tissue. It is guaranteed to work for IHC, WB in mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-13
Question
We ordered your anti-EGF antibody for IHC on kidney last year. I am using mouse, and We are going to use the antibody for WB next. My lab would like examining kidney as well as colon in our next experiment. Could give a recommendation on which antibody would work the best for WB?
R. Jones
Verified customer
Asked: 2019-09-24
Answer
I have checked the website and datasheets of our anti-EGF antibody and it seems that PA1398 has been tested on mouse in both IHC and WB. Thus PA1398 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-09-24
Question
See attached the WB image, lot number and protocol we used for renal medulla using anti-EGF antibody PA1398. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-09
Question
My question regarding product PA1398, anti-EGF antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1398 anti-EGF antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-03
Question
Is a blocking peptide available for product anti-EGF antibody (PA1398)?
Verified Customer
Verified customer
Asked: 2019-08-20
Answer
We do provide the blocking peptide for product anti-EGF antibody (PA1398). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-20
Question
We have seen staining in mouse teratocarcinoma. What should we do? Is anti-EGF antibody supposed to stain teratocarcinoma positively?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
From literature teratocarcinoma does express EGF. From Uniprot.org, EGF is expressed in renal medulla, kidney, teratocarcinoma, colon, among other tissues. Regarding which tissues have EGF expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Kidney, Pubmed ID: 3491360
Teratocarcinoma, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-06-26
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for renal medulla using anti-EGF antibody PA1398. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-10
Question
Do you have a BSA free version of anti-EGF antibody PA1398 available?
Verified Customer
Verified customer
Asked: 2018-09-24
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-EGF antibody PA1398 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-09-24
Question
I see that the anti-EGF antibody PA1398 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-04-03
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-04-03
Question
I am interested in to test anti-EGF antibody PA1398 on mouse renal medulla for research purposes, then I may be interested in using anti-EGF antibody PA1398 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2017-11-14
Answer
The products we sell, including anti-EGF antibody PA1398, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-11-14
Question
I was wanting to use your anti-EGF antibody for IHC for mouse renal medulla on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse renal medulla identification?
N. Johnson
Verified customer
Asked: 2017-10-26
Answer
It shows on the product datasheet, PA1398 anti-EGF antibody has been tested for IHC, WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse renal medulla in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-26
Question
My boss were content with the WB result of your anti-EGF antibody. However we have observed positive staining in kidney membrane using this antibody. Is that expected? Could you tell me where is EGF supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-06-05
Answer
According to literature, kidney does express EGF. Generally EGF expresses in membrane. Regarding which tissues have EGF expression, here are a few articles citing expression in various tissues:
Colon, Pubmed ID: 15489334
Kidney, Pubmed ID: 3491360
Teratocarcinoma, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-06-05
Question
Does anti-EGF antibody PA1398 work for IHC with renal medulla?
N. Huang
Verified customer
Asked: 2015-09-18
Answer
According to the expression profile of renal medulla, EGF is highly expressed in renal medulla. So, it is likely that anti-EGF antibody PA1398 will work for IHC with renal medulla.
Boster Scientific Support
Answered: 2015-09-18
Question
Will PA1398 anti-EGF antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
L. Rodriguez
Verified customer
Asked: 2013-12-06
Answer
As indicated on the product datasheet, PA1398 anti-EGF antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-12-06
