Click image to see more details
Product Info Summary
| SKU: | A00946 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P |
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Product info
Product Name
Anti-ENA-78 CXCL5 Antibody
SKU/Catalog Number
A00946
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ENA-78 CXCL5 Antibody catalog # A00946. Tested in IHC-P applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ENA-78 CXCL5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00946)
Host
Rabbit
Contents
PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic peptide from human protein at AA range: 65-114.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00946 is reactive to CXCL5 in Human
Applications
A00946 is guaranteed for IHC-P Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
11.972kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC-P, 1:50-300
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human-pancreas-cancer, antibody was diluted at 1:200.
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Immunohistochemical analysis of paraffin-embedded human-pancreas-cancer, antibody was diluted at 1:200.
Protein Target Info & Infographic
Gene/Protein Information For CXCL5 (Source: Uniprot.org, NCBI)
Gene Name
CXCL5
Full Name
C-X-C motif chemokine 5
Weight
11.972kDa
Superfamily
intercrine alpha (chemokine CxC) family
Alternative Names
LIX CXCL5 ENA-78, SCYB5 C-X-C motif chemokine ligand 5 C-X-C motif chemokine 5|chemokine (C-X-C motif) ligand 5|epithelial-derived neutrophil-activating protein 78|neutrophil-activating peptide ENA-78|neutrophil-activating protein 78|small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CXCL5, check out the CXCL5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CXCL5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ENA-78 CXCL5 Antibody (A00946)
Hello CJ!
No publications found for A00946
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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15 Customer Q&As for Anti-ENA-78 CXCL5 Antibody
Question
I was wanting to use your anti-ENA-78 antibody for IHC-P for human smooth muscle tissue on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human smooth muscle tissue identification?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
As indicated on the product datasheet, A00946 anti-ENA-78 antibody has been validated for IHC-P on human tissues. We have an innovator award program that if you test this antibody and show it works in human smooth muscle tissue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-05-06
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for smooth muscle tissue using anti-ENA-78 antibody A00946. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-27
Question
My question regarding product A00946, anti-ENA-78 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00946 anti-ENA-78 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-27
Question
Does A00946 anti-ENA-78 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-14
Answer
As indicated on the product datasheet, A00946 anti-ENA-78 antibody as been tested on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-14
Question
Is this A00946 anti-ENA-78 antibody reactive to the isotypes of CXCL5?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
The immunogen of A00946 anti-ENA-78 antibody is Synthetic peptide from human protein at AA range: 65-114. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-02
Question
See attached the WB image, lot number and protocol we used for smooth muscle tissue using anti-ENA-78 antibody A00946. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-11
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-11
Question
Would anti-ENA-78 antibody A00946 work on canine IHC-P with smooth muscle tissue?
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
Our lab technicians have not validated anti-ENA-78 antibody A00946 on canine. You can run a BLAST between canine and the immunogen sequence of anti-ENA-78 antibody A00946 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine smooth muscle tissue in IHC-P, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-07
Question
Is there a BSA free version of anti-ENA-78 antibody A00946 available?
Verified Customer
Verified customer
Asked: 2019-03-07
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-ENA-78 antibody A00946 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-03-07
Question
We have been able to see staining in human epithelium. Are there any suggestions? Is anti-ENA-78 antibody supposed to stain epithelium positively?
Verified Customer
Verified customer
Asked: 2019-02-07
Answer
Based on literature epithelium does express CXCL5. Based on Uniprot.org, CXCL5 is expressed in smooth muscle tissue, kidney, epithelium, peripheral blood monocyte, platelet, among other tissues. Regarding which tissues have CXCL5 expression, here are a few articles citing expression in various tissues:
Epithelium, Pubmed ID: 1744577
Kidney, Pubmed ID: 15489334
Peripheral blood monocyte, Pubmed ID: 10095777
Platelet, Pubmed ID: 12665801
Boster Scientific Support
Answered: 2019-02-07
Question
My question regards to test anti-ENA-78 antibody A00946 on human smooth muscle tissue for research purposes, then I may be interested in using anti-ENA-78 antibody A00946 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
V. Huang
Verified customer
Asked: 2019-01-18
Answer
The products we sell, including anti-ENA-78 antibody A00946, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-01-18
Question
Will anti-ENA-78 antibody A00946 work for IHC-P with smooth muscle tissue?
Verified Customer
Verified customer
Asked: 2018-06-15
Answer
According to the expression profile of smooth muscle tissue, CXCL5 is highly expressed in smooth muscle tissue. So, it is likely that anti-ENA-78 antibody A00946 will work for IHC-P with smooth muscle tissue.
Boster Scientific Support
Answered: 2018-06-15
Question
We are currently using anti-ENA-78 antibody A00946 for human tissue, and we are well pleased with the IHC-P results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
R. Thomas
Verified customer
Asked: 2017-09-25
Answer
The anti-ENA-78 antibody (A00946) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-25
Question
I see that the anti-ENA-78 antibody A00946 works with IHC-P, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-06-09
Answer
You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-06-09
Question
My boss were satisfied with the WB result of your anti-ENA-78 antibody. However we have been able to see positive staining in kidney secreted. using this antibody. Is that expected? Could you tell me where is CXCL5 supposed to be expressed?
R. Edwards
Verified customer
Asked: 2015-01-12
Answer
From what I have seen in literature, kidney does express CXCL5. Generally CXCL5 expresses in secreted. Regarding which tissues have CXCL5 expression, here are a few articles citing expression in various tissues:
Epithelium, Pubmed ID: 1744577
Kidney, Pubmed ID: 15489334
Peripheral blood monocyte, Pubmed ID: 10095777
Platelet, Pubmed ID: 12665801
Boster Scientific Support
Answered: 2015-01-12
Question
Is a blocking peptide available for product anti-ENA-78 antibody (A00946)?
T. Dhar
Verified customer
Asked: 2013-01-03
Answer
We do provide the blocking peptide for product anti-ENA-78 antibody (A00946). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2013-01-03
