Anti-Enolase 1 ENO1 Monoclonal Antibody

Product Name

Anti-Enolase 1 ENO1 Monoclonal Antibody

View all Enolase 1 Antibodies

SKU/Catalog Number

M01250-1

Size

100ul

Description

Boster Bio Anti-Enolase 1 ENO1 Monoclonal Antibody catalog # M01250-1. Tested in IF, IHC, WB applications. This antibody reacts with Bovine, Equine, Human, Mouse, Pig, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Enolase 1 ENO1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01250-1)

Host

Mouse

Contents

Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN₃

Clonality

Monoclonal

Immunogen

N-terminal 12 amino acids of bovine enolase 1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01250-1 is reactive to ENO1 in Bovine, Equine, Human, Mouse, Pig, Rat

Applications

M01250-1 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

47.169kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:5,000-1:10,000 IF/ICC and IHC: 1:2,000-1:5,000.

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of different cell lysates using mouse mAb to -enolase, M01250-1, dilution 1:10,000 in green: [1] protein standard (red), [2] NIH-3T3 l, [3] C6, [4] HEK293, [5] HeLa, and [6] SH-SY5Y cells. A strong single band at 47kDa corresponds to the -enolase protein.

Click image to see more details

Immunofluorescent analysis of HeLa cells stained with mouse mAb to -enolase, M01250-1, dilution 1:500 in green and costained with chicken pAb to HSP60, dilution 1:5,000, in red. The blue is DAPI staining of nuclear DNA. The M01250-1 antibody reveals strong cytoplasmic staining while the other antibody specifically labels mitochondria in these cells.

Gene/Protein Information For ENO1 (Source: Uniprot.org, NCBI)

Alternative Names

2-phospho-D-glycerate hydro-lyase; Alpha enolase; C-myc promoter-binding protein; c-myc promoter-binding protein-1; EC 4.2.1; EC 4.2.1.11; ENO1L1; Enolase 1; enolase 1, (alpha); MBP-1; MBPB1; MPB-1; MPB1c-myc promoter-binding protein 1; MYC promoter-binding protein 1; NNE; Non-neural enolase; Phosphopyruvate hydratase; Plasminogen-binding protein; PPHalpha enolase like 1; tau-crystallin ENO1 ENO1L1, HEL-S-17, MPB1, NNE, PPH enolase 1 alpha-enolase|c-myc promoter-binding protein-1|2-phospho-D-glycerate hydro-lyase|MYC promoter-binding protein 1|alpha enolase like 1|enolase 1, (alpha)|enolase-alpha|epididymis secretory protein Li 17|non-neural enolase|phosphopyruvate hydratase|plasminogen-binding protein|tau-crystallin

For more info on ENO1, check out the ENO1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ENO1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the ENO1 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis