Anti-Estrogen Receptor beta Rabbit Monoclonal Antibody

SKU M00786-1
Size 100ug/vial
Reactivity Human
Clonality Monoclonal
Clone Number IDI-5
Host Rabbit
Applications IHC, WB

Overview

Product Name Anti-Estrogen Receptor beta Rabbit Monoclonal Antibody
SKU/Catalog Number M00786-1
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
Size 100ug/vial
Description Anti-Estrogen Receptor beta Rabbit Monoclonal Antibodytested for IHC, WB in Human
Cite This Product Anti-Estrogen Receptor beta Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00786-1)
Host Rabbit
Immunogen A synthesized peptide derived from human Estrogen Receptor beta
Reactivity Human

Assay Details

Assay Dilutions Overview

WB 1:500-1:2000
IHC 1:50-1:200

Images And Assay Conditions

/M/0/M00786 1 ESR2 primary antibodies WB testing 1.jpg

Western blot analysis of Estrogen Receptor beta expression in MCF-7 Cell Lysate (M00786-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESR2 monoclonal antibody (Catalog # M00786-1) overnight at 4℃, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESR2

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q92731
Gene Name ESR2
Protein Name Estrogen receptor beta
Tissue Specificity Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.
Alternative Names Estrogen receptor beta;ER-beta;Nuclear receptor subfamily 3 group A member 2;ESR2;ESTRB, NR3A2;
Subcellular Localization Nucleus .
Molecular Weight 59216 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. .

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Buy primary get secondary antibody for free.
Option Price
100ug $299
100ug+Free HRP Secondary BA1054 $299
100ug+Free Biotin Secondary BA1003 $299
30ug sample size $99

USD $299

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