Overview
| Product Name |
Anti-Estrogen Receptor beta ESR2 Rabbit Monoclonal Antibody
See more ER beta/NR3A2 products |
| Catalog# |
M00786-1 |
| Pack Size |
100ug/vial |
| Storage & Handling |
At -20°C for one year. Avoid repeated freezing and thawing. |
| Description |
Monoclonal antibody for ESTROGEN RECEPTOR BETA/ESR2 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC, WB. Reactive species: Human ESTROGEN RECEPTOR BETA/ESR2 information: Molecular Weight: 59216 MW; Subcellular Localization: Nucleus ; Tissue Specificity: Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expresse |
| Cite This Product |
Anti-Estrogen Receptor beta ESR2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00786-1)
|
| Antibodies Validation |
Antibodies Validation Information
|
Product Specs
| Host |
Rabbit |
| Reactivity/Species |
Human |
| Applications |
WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Immunogen |
A synthesized peptide derived from human Estrogen Receptor beta |
Gene/Protein Basic Information For ESR2 (Source: Uniprot.org, NCBI)
| Uniprot Id | Q92731 |
|---|
| NCBI Gene Id | 2100 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | ESR2 |
|---|
| Protein Name | Estrogen receptor beta |
|---|
| Superfamily | nuclear hormone receptor family |
|---|
| Alternative Names | ER beta/NR3A2|ER beta; Erb; ER-beta; ESR2; ESR-BETA; ESTRB; estrogen receptor 2 (ER beta); estrogen receptor beta 4; estrogen receptor beta; NR3A2; NR3A2ESRB; Nuclear receptor subfamily 3 group A member 2 |
|---|
| Molecular Weight | 59216 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ESR2, check out the ESR2 Infographic
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Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for ESR2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the ESR2 infographic
Our Boster Quality Guarantee for Anti-Estrogen Receptor beta ESR2 Rabbit Monoclonal Antibody covers its use in the following applications.
WB 1:500-1:2000
IHC 1:50-1:200
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Western blot analysis of Estrogen Receptor beta expression in MCF-7 Cell Lysate (M00786-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESR2 monoclonal antibody (Catalog # M00786-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESR2
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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