Overview
| Product Name |
Anti-Estrogen Receptor beta ESR2 Rabbit Monoclonal Antibody
See all ER beta/NR3A2 primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
M00786-1 |
| Pack Size |
100ug/vial |
| Storage & Handling |
At -20°C for one year. Avoid repeated freezing and thawing. |
| Description |
Monoclonal antibody for ESTROGEN RECEPTOR BETA/ESR2 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC, WB. Reactive species: Human ESTROGEN RECEPTOR BETA/ESR2 information: Molecular Weight: 59216 MW; Subcellular Localization: Nucleus ; Tissue Specificity: Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expresse |
| Cite This Product |
Anti-Estrogen Receptor beta ESR2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00786-1)
|
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Anti-Estrogen Receptor beta ESR2 Rabbit Monoclonal Antibody may replace the following items: sc 79772|sc 79769. |
Product Specs
| Host |
Rabbit |
| Reactivity/Species |
Human |
| Applications |
WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Immunogen |
A synthesized peptide derived from human Estrogen Receptor beta |
| Cross Reactivity |
No cross reactivity with other proteins |
Gene/Protein Basic Information For ESR2 (Source: Uniprot.org, NCBI)
| Uniprot Id | Q92731 |
|---|
| NCBI Gene Id | 2100 |
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| Species Of This Entry | Human |
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| Gene Name | ESR2 |
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| Protein Name | Estrogen receptor beta |
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| Superfamily | nuclear hormone receptor family |
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| Alternative Names | ER beta/NR3A2|ER beta; Erb; ER-beta; ESR2; ESR-BETA; ESTRB; estrogen receptor 2 (ER beta); estrogen receptor beta 4; estrogen receptor beta; NR3A2; NR3A2ESRB; Nuclear receptor subfamily 3 group A member 2 |
|---|
| Molecular Weight | 59216 |
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| Post Tranlational Modifications | Phosphorylation; Methylation; Demethylation; Hydroxylation |
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| Subcellular Localization | Nucleus. |
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| Gene Location | 14q23.2-q23.3, on Chromasome 14, gene sequence: NC_000014.9 (64226707..64338631, complement) |
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*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For ESR2 (Source: Uniprot.org, NCBI)
| Protein Function | Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA-binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual. |
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| Tissue Specificity | Isoform 1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform 2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform 4 is found in testis. Isoform 5 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform 6 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain. |
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| Interacting Proteins | BRCA1; CCDC62; CCNDBP1; CTGF; EGFR; FOXO3; MDFI; Ncor1; PLSCR1; PPP5C; TFCP2 |
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| Research Areas | Breast Cancer; Cancer; GPCR |
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| Related Diseases | Malignant Neoplasms; Malignant Neoplasm Of Breast; Mammary Neoplasms; Neoplasms; Infertility; Cardiovascular Diseases; Osteoporosis; Endometriosis, Site Unspecified; Dysequilibrium Syndrome; Pituitary Diseases; Malignant Neoplasm Of Prostate; Fracture; Cholangiocarcinoma; Colorectal Cancer; Hypertensive Disease; Carcinogenesis; Osteoporosis, Postmenopausal; Insulin Resistance; Obesity; Endometrial Polyp |
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| Related Pathways | Pathogenesis; Cell Proliferation; Localization; Ovulation; Secretion; Spermatogenesis; Aging; Menopause; Methylation; Transport; Menarche; Cell Division; Cotyledon Development; Dna Methylation; Regeneration; Sertoli Cell Proliferation; Cell Growth; Angiogenesis; Sex Determination; Cognition |
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| Scientific References | PMID: 9473491 by Ogawa S., et al. The complete primary structure of human estrogen receptor beta (hERbeta) and its heterodimerization with ER alpha in vivo and in vitro.
PMID: 9636657 by Moore J.T., et al. Cloning and characterization of human estrogen receptor beta isoforms. |
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Our Boster Quality Guarantee for Anti-Estrogen Receptor beta ESR2 Rabbit Monoclonal Antibody covers its use in the following applications.
WB 1:500-1:2000
IHC 1:50-1:200
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Western blot analysis of Estrogen Receptor beta expression in MCF-7 Cell Lysate (M00786-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ESR2 monoclonal antibody (Catalog # M00786-1) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ESR2
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all ER beta/NR3A2 primary antibodies,
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