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Product Info Summary
| SKU: | CI1042 |
|---|---|
| Size: | 50μg/50μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ChIP, ChIP-seq, IF, WB |
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Product info
Product Name
Anti-EZH2/Kmt6 Antibody
SKU/Catalog Number
CI1042
Size
50μg/50μl
Form
Liquid
Description
Boster Bio Anti-EZH2/Kmt6 Antibody (Catalog# CI1042). Tested in ChIP, ChIP-seq, WB, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C. For long-term storage, store at -80°C. Avoid multiple freeze-thaw cycles.
Cite This Product
Anti-EZH2/Kmt6 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # CI1042)
Host
Rabbit
Contents
Protein G purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.
Clonality
Polyclonal
Immunogen
This antibody is raised in rabbit against the N-terminus (aa1-343) of the mouse EZH2 protein (Enhancer of zeste homolog 2).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
CI1042 is reactive to EZH2 in Human, Mouse
Applications
CI1042 is guaranteed for ChIP, ChIP-seq, IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
85.363kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
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ChIP assays were performed using K562 cells, Anti-EZH2 polyclonal antibody (Catalog # CI1042) and optimized PCR primer sets for qPCR. A titration of the antibody consisting of 1, 2, 5 and 10 μg per ChIP experiment was analysed. IgG (2 μg/IP) was used as negative IP control. Quantitative PCR was performed with primers for MYT1 and HOXA9, used as positive control targets, and for the coding regions of the active CCT5 and EIF2S3 genes, used as negative controls.
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ChIP was performed on sheared chromatin from 4 million K562 cells using 2 μg of Anti-EZH2 polyclonal antibody (Catalog # CI1042). The IP DNA was subsequently analysed on an Illumina HiSeq. Library preparation, cluster generation and sequencing were performed according to the manufacturer instructions. The 51 bp tags were aligned to the human genome using the BWA algorithm. Figure 2 shows the peak distribution along the short arm and a 6 Mb region containing several enriched regions of human chromosome 3 (figure 2A and B, respectively), and in two genomic regions containing the MYT1 gene on chromosome 20 and the HOX cluster on chromosome 7 (figure 2C and D).
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Western blot analysis of EZH2 expression in nuclear extracts from HeLa cells (40
Protein Target Info & Infographic
Gene/Protein Information For EZH2 (Source: Uniprot.org, NCBI)
Gene Name
EZH2
Full Name
Histone-lysine N-methyltransferase EZH2
Weight
85.363kDa
Superfamily
class V-like SAM-binding methyltransferase superfamily
Alternative Names
EC 2.1.1; EC 2.1.1.43; enhancer of zeste (Drosophila) homolog 2; enhancer of zeste 2; enhancer of zeste homolog 2 (Drosophila); Enhancer of zeste homolog 2; ENX1; ENX-1KMT6EZH1KMT6A; EZH2; histone-lysine N-methyltransferase EZH2; KMT6; Lysine N-methyltransferase 6; MGC9169 EZH2 ENX-1, ENX1b, KMT6, KMT6A, WVS, WVS2, EZH2 enhancer of zeste 2 polycomb repressive complex 2 subunit histone-lysine N-methyltransferase EZH2|enhancer of zeste homolog 2|lysine N-methyltransferase 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on EZH2, check out the EZH2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for EZH2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-EZH2/Kmt6 Antibody (CI1042)
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No publications found for CI1042
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-EZH2/Kmt6 Antibody
Question
Do you have a BSA free version of anti-EZH2/Kmt6 antibody CI1042 available?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-EZH2/Kmt6 antibody CI1042 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-09
Question
We have observed staining in mouse brain testis. Are there any suggestions? Is anti-EZH2/Kmt6 antibody supposed to stain brain testis positively?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
From what I have seen in literature brain testis does express EZH2. From what I have seen in Uniprot.org, EZH2 is expressed in lung, brain, brain testis, pancreas, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have EZH2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8954776
Brain, and Testis, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-04-06
Question
Would anti-EZH2/Kmt6 antibody CI1042 work for ChIP with lung?
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
According to the expression profile of lung, EZH2 is highly expressed in lung. So, it is likely that anti-EZH2/Kmt6 antibody CI1042 will work for ChIP with lung.
Boster Scientific Support
Answered: 2020-03-16
Question
I have a question about product CI1042, anti-EZH2/Kmt6 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free CI1042 anti-EZH2/Kmt6 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-21
Question
Is this CI1042 anti-EZH2/Kmt6 antibody reactive to the isotypes of EZH2?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
The immunogen of CI1042 anti-EZH2/Kmt6 antibody is This antibody is raised in rabbit against the N-terminus (aa1-343) of the mouse EZH2 protein (Enhancer of zeste homolog 2). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-15
Question
I see that the anti-EZH2/Kmt6 antibody CI1042 works with ChIP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
You can find protocols for ChIP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-11-07
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-EZH2/Kmt6 antibody CI1042. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-29
Question
My lab would like to test anti-EZH2/Kmt6 antibody CI1042 on human lung for research purposes, then I may be interested in using anti-EZH2/Kmt6 antibody CI1042 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
The products we sell, including anti-EZH2/Kmt6 antibody CI1042, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-30
Question
I was wanting to use your anti-EZH2/Kmt6 antibody for ChIP for human lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lung identification?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
You can see on the product datasheet, CI1042 anti-EZH2/Kmt6 antibody has been validated for ChIP on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-15
Question
Is a blocking peptide available for product anti-EZH2/Kmt6 antibody (CI1042)?
L. Johnson
Verified customer
Asked: 2019-07-16
Answer
We do provide the blocking peptide for product anti-EZH2/Kmt6 antibody (CI1042). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-16
Question
My boss were happy with the WB result of your anti-EZH2/Kmt6 antibody. However we have seen positive staining in brain testis nucleus using this antibody. Is that expected? Could you tell me where is EZH2 supposed to be expressed?
G. Huang
Verified customer
Asked: 2019-01-11
Answer
According to literature, brain testis does express EZH2. Generally EZH2 expresses in nucleus. Regarding which tissues have EZH2 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 8954776
Brain, and Testis, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 16964243, 17081983, 18220336, 18669648, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 19690332
Pancreas, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-01-11
Question
See attached the WB image, lot number and protocol we used for lung using anti-EZH2/Kmt6 antibody CI1042. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-10-08
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-08
Question
Does CI1042 anti-EZH2/Kmt6 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-01-23
Answer
As indicated on the product datasheet, CI1042 anti-EZH2/Kmt6 antibody as been tested on ChIP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-01-23
Question
I was wanting to use using your anti-EZH2/Kmt6 antibody for negative regulation of retinoic acid receptor signaling pathway studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
J. Baker
Verified customer
Asked: 2016-01-18
Answer
I appreciate your inquiry. This CI1042 anti-EZH2/Kmt6 antibody is tested on k562 cells, hela cells. It is guaranteed to work for ChIP in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-01-18
Question
We are currently using anti-EZH2/Kmt6 antibody CI1042 for human tissue, and we are content with the ChIP results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on dog tissues as well?
J. Jones
Verified customer
Asked: 2014-10-08
Answer
The anti-EZH2/Kmt6 antibody (CI1042) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-10-08
