Anti-GAD65/GAD2 Antibody

GAD2/GAD65 antibody

Boster Bio Anti-GAD65/GAD2 Antibody catalog # PA1403. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: PA1403
Size: 100 μg/vial
Reactive Species: Mouse, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-GAD65/GAD2 Antibody

View all GAD2/GAD65 Antibodies

SKU/Catalog Number

PA1403

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GAD65/GAD2 Antibody catalog # PA1403. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GAD65/GAD2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1403)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human GAD65, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1403 is reactive to GAD2 in Mouse, Rat

Applications

PA1403 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

65 kDa

Calculated molecular weight

65.411kDa

Background of GAD2/GAD65

Glutamate decarboxylase 2, also known as GAD65, is an enzyme that in humans is encoded by the GAD2 gene. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Rat, By Heat

Validation Images & Assay Conditions

Gene/Protein Information For GAD2 (Source: Uniprot.org, NCBI)

Gene Name

GAD2

Full Name

Glutamate decarboxylase 2

Weight

65.411kDa

Superfamily

group II decarboxylase family

Alternative Names

EC 4.1.1; EC 4.1.1.15; GAD2; GAD65; GAD-65; GAD65MGC161605; glutamate decarboxylase 2 (pancreatic islets and brain, 65kD); glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); glutamate decarboxylase 2; Glutamate decarboxylase 65 kDa isoform; Glutamate decarboxylase-2 (pancreas); MGC161607,65 kDa glutamic acid decarboxylase GAD2 GAD65 glutamate decarboxylase 2 glutamate decarboxylase 2|65 kDa glutamic acid decarboxylase|GAD-65|Glutamate decarboxylase-2 (pancreas)|glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GAD2, check out the GAD2 Infographic

GAD2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GAD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1403 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Acrylamide neurotoxicity on the cerebrum of weaning rats

Shi J, Ma Y, Zheng M, Ruan Z, Liu J, Tian S, Zhang D, He X, Li G. Toxicol Ind Health. 2012 Feb;28(1):10-20. Doi: 10.1177/0748233711401264. Epub 2011 Mar 28. Effect Of Sub-Acute Exposure To Acrylamide On Gabaergic Neurons And Astrocytes In Weaning ...

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6 Customer Q&As for Anti-GAD65/GAD2 Antibody

Question

Is this PA1403 anti-GAD65/GAD2 antibody reactive to the isotypes of GAD2?

Verified Customer

Verified customer

Asked: 2019-09-25

Answer

The immunogen of PA1403 anti-GAD65/GAD2 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human GAD65(21-37aa ENPGTARAWCQVAQKFT), identical to the related mouse and rat sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-09-25

Question

We are currently using anti-GAD65/GAD2 antibody PA1403 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-30

Answer

The anti-GAD65/GAD2 antibody (PA1403) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-30

Question

Is a blocking peptide available for product anti-GAD65/GAD2 antibody (PA1403)?

Verified Customer

Verified customer

Asked: 2019-07-02

Answer

We do provide the blocking peptide for product anti-GAD65/GAD2 antibody (PA1403). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-02

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for pancreatic islet using anti-GAD65/GAD2 antibody PA1403. Let me know if you need anything else.

E. Edwards

Verified customer

Asked: 2015-12-04

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-12-04

Question

Can you help my question with product PA1403, anti-GAD65/GAD2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

M. Anderson

Verified customer

Asked: 2014-01-23

Answer

It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1403 anti-GAD65/GAD2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-01-23

Question

I was wanting to use your anti-GAD65/GAD2 antibody for WB for human pancreatic islet on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human pancreatic islet identification?

O. Dhar

Verified customer

Asked: 2013-10-04

Answer

You can see on the product datasheet, PA1403 anti-GAD65/GAD2 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human pancreatic islet in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-10-04

Order DetailsPrice
PA1403

100μg

$370
PA1403-10ug

10μg sample (liquid)

$99
PA1403-Biotin

100 μg Biotin conjugated

$570
PA1403-Cy3

100 μg Cy3 conjugated

$570
PA1403-Dylight488

100 μg Dylight488 conjugated

$570
PA1403-Dylight550

100 μg Dylight550 conjugated

$570
PA1403-Dylight594

100 μg Dylight594 conjugated

$570
PA1403-FITC

100 μg FITC conjugated

$570
PA1403-HRP

100 μg HRP conjugated

$570
PA1403-APC

100 μg APC conjugated

$670
PA1403-PE

100 μg PE conjugated

$670
PA1403-carrier-free

Carrier Free

$370

More conjugates/formats

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PA1403
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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