Anti-Glutaminase GLS Rabbit Monoclonal Antibody

Product Name

Anti-Glutaminase GLS Rabbit Monoclonal Antibody

View all Glutaminase Antibodies

SKU/Catalog Number

M01272

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Glutaminase GLS Rabbit Monoclonal Antibody catalog # M01272. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Glutaminase GLS Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01272)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

FIA-7

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Glutaminase

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M01272 is reactive to GLS in Human, Mouse, Rat

Applications

M01272 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

70 kDa

Calculated molecular weight

73.461kDa

Background of Glutaminase

Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

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Western blot analysis of Glutaminase expression in 293T cell lysate.

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Immunohistochemical analysis of paraffin-embedded human brain, using Glutaminase Antibody.

Gene/Protein Information For GLS (Source: Uniprot.org, NCBI)

Alternative Names

AAD20; EC 3.5.1.2; GLS; GLS1DKFZp686O15119; glutaminase C; glutaminase kidney isoform, mitochondrial; Glutaminase; glutaminase, phosphate-activated; K-Glutaminase; KIAA0838FLJ10358; L-Glutamine Amidohydrolase GLS AAD20, CASGID, DEE71, EIEE71, GAC, GAM, GDPAG1, KGA, GLS glutaminase glutaminase kidney isoform, mitochondrial|K-glutaminase|L-glutamine amidohydrolase|glutaminase C|glutaminase, phosphate-activated

For more info on GLS, check out the GLS Infographic

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In this infographic, you will see the following information for GLS: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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