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Product Info Summary
| SKU: | PA2012 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Hamartin/TSC1 Antibody
SKU/Catalog Number
PA2012
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Hamartin/TSC1 Antibody catalog # PA2012. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hamartin/TSC1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2012)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Hamartin, identical to the related rat sequence, and different from the related mouse sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2012 is reactive to TSC1 in Human, Mouse, Rat
Applications
PA2012 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
160 kDa
Calculated molecular weight
129.767kDa
Background of TSC1
TSC1 (Tuberous Sclerosis 1), also called HAMARTIN or TSC, is a human protein and gene. As part of a comprehensive strategy to identify the gene mutant in tuberous sclerosis-1, van Slegtenhorst et al. (1997) developed an overlapping contig of clones from the 1.4-Mb TSC1 region on chromosome 9. Benvenuto et al. (2000) showed that overexpression of the TSC1 gene in rat fibroblasts inhibits growth and causes changes in cell morphology. Van Slegtenhorst et al. (1998) showed that hamartin and tuberin associate physically in vivo, however, and that the interaction is mediated by predicted coiled-coil domains.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Validation Images & Assay Conditions
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Anti-Hamartin antibody, PA2012, Western blotting
All lanes: Anti Hamartin (PA2012) at 0.5ug/ml
Lane 1: Rat Skeletal Muscle Tissue Lysate at 50ug
Lane 2: Rat Heart Tissue Lysate at 50ug
Lane 3: Rat Brain Tissue Lysate at 50ug
Lane 4: Rat Lung Tissue Lysate at 50ug
Lane 5: 293T Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Lane 7: HT1080 Whole Cell Lysate at 40ug
Lane 8: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 130KD
Observed bind size: 160KD
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Anti-Hamartin antibody, PA2012, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Anti-Hamartin antibody, PA2012, IHC(P)
IHC(P): Rat Brain Tissue
Protein Target Info & Infographic
Gene/Protein Information For TSC1 (Source: Uniprot.org, NCBI)
Gene Name
TSC1
Full Name
Hamartin
Weight
129.767kDa
Alternative Names
Hamartin; KIAA0243MGC86987; LAMhamartin; TSC; TSC1; Tuberous sclerosis 1 protein; tuberous sclerosis 1; tumor suppressor TSC1 LAM, TSC TSC complex subunit 1 hamartin|truncated hemartin|tuberous sclerosis 1 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TSC1, check out the TSC1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TSC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hamartin/TSC1 Antibody (PA2012)
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No publications found for PA2012
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Hamartin/TSC1 Antibody
Question
We were well pleased with the WB result of your anti-Hamartin/TSC1 antibody. However we have seen positive staining in cerebellum cytoplasm. using this antibody. Is that expected? Could you tell me where is TSC1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
According to literature, cerebellum does express TSC1. Generally TSC1 expresses in cytoplasm. Regarding which tissues have TSC1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 9924605, 10570911
Blood, and Lymphoblast, Pubmed ID: 10533067
Bone marrow, Pubmed ID: 9039502
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Peripheral blood, Pubmed ID: 9328481, 10227394
Peripheral blood leukocyte, Pubmed ID: 10607950, 10874311, 12112044
Platelet, Pubmed ID: 18088087
Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-10-15
Question
We have been able to see staining in human cervix carcinoma. Are there any suggestions? Is anti-Hamartin/TSC1 antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2018-07-05
Answer
From literature cervix carcinoma does express TSC1. From Uniprot.org, TSC1 is expressed in cerebellum, testis, bone marrow, embryonic kidney, platelet, cervix carcinoma, leukemic t-cell, cervix carcinoma erythroleukemia, liver, peripheral blood, blood, blood lymphoblast, peripheral blood leukocyte, among other tissues. Regarding which tissues have TSC1 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 9924605, 10570911
Blood, and Lymphoblast, Pubmed ID: 10533067
Bone marrow, Pubmed ID: 9039502
Cervix carcinoma, Pubmed ID: 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Peripheral blood, Pubmed ID: 9328481, 10227394
Peripheral blood leukocyte, Pubmed ID: 10607950, 10874311, 12112044
Platelet, Pubmed ID: 18088087
Testis, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-07-05
Question
We are currently using anti-Hamartin/TSC1 antibody PA2012 for human tissue, and we are content with the IHC-P results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2018-05-17
Answer
The anti-Hamartin/TSC1 antibody (PA2012) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-05-17
Question
I would like using your anti-Hamartin/TSC1 antibody for protein stabilization studies. Has this antibody been tested with western blotting on 293t whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-03-28
Answer
I appreciate your inquiry. This PA2012 anti-Hamartin/TSC1 antibody is validated on rat heart tissue, skeletal muscle tissue, tissue lysate, brain tissue, lung tissue, 293t whole cell lysate, hela whole cell lysate, ht1080 whole cell lysate, smmc whole cell lysate, mammary cancer tissue. It is guaranteed to work for IHC-P, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-03-28
Question
We ordered your anti-Hamartin/TSC1 antibody for WB on cerebellum in the past. I am using mouse, and We intend to use the antibody for IHC-P next. We need examining cerebellum as well as bone marrow in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?
K. Baker
Verified customer
Asked: 2018-02-05
Answer
I took a look at the website and datasheets of our anti-Hamartin/TSC1 antibody and it appears that PA2012 has been validated on mouse in both WB and IHC-P. Thus PA2012 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-02-05
