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Product Info Summary
| SKU: | M00137-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IHC-P |
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Product info
Product Name
Anti-Hu CD14 Purified Monoclonal Antibody
SKU/Catalog Number
M00137-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Hu CD14 Purified Monoclonal Antibody (Catalog# M00137-1). Tested in ELISA, IHC-P, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Hu CD14 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00137-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
B-A8
Isotype
Mouse IgG1
Immunogen
Human monocytes. The mouse monoclonal antibody B-A8 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked extracellular membrane glycoprotein expressed on the surface of monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00137-1 is reactive to CD14 in Human
Applications
M00137-1 is guaranteed for ELISA, Flow Cytometry, IHC-P Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
40.076kDa
Background of CD14
CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where it serves as a multifunctional lipopolysaccharide receptor. It is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: The antibody B-A8 has been tested as the capture antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody MEM-18 (cat. no. 1B-212-C100). Flow cytometry: 1 μg/ml; Immunohistochemistry (paraffin sections): 2-10 μg/ml, positive tissue: PML brain sections; positive staining on human PML brain sections was mainly observed on monocytes in the lumenal side of brain blood vessels, and on some perivascular cells adjacent to medium-sized vessels. Heat retrieval of antigen is recommeded.
Validation Images & Assay Conditions
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Protein Target Info & Infographic
Gene/Protein Information For CD14 (Source: Uniprot.org, NCBI)
Gene Name
CD14
Full Name
Monocyte differentiation antigen CD14
Weight
40.076kDa
Alternative Names
CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein CD14 CD14 molecule monocyte differentiation antigen CD14|myeloid cell-specific leucine-rich glycoprotein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD14, check out the CD14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Hu CD14 Purified Monoclonal Antibody (M00137-1)
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No publications found for M00137-1
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