Anti-human CD14 Monoclonal Antibody APC Conjugated, Flow Validated

Boster Bio Anti-human CD14 Monoclonal Antibody APC Conjugated, Flow Validated catalog # FC00137-APC. Tested in Flow Cytometry applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: FC00137-APC
Size: 100 Tests, 200 Tests
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry

Product Name

Anti-human CD14 Monoclonal Antibody APC Conjugated, Flow Validated

See all CD14 products

SKU/Catalog Number

FC00137-APC

Size

100 Tests, 200 Tests

Form

Liquid

Description

Boster Bio Anti-human CD14 Monoclonal Antibody APC Conjugated, Flow Validated catalog # FC00137-APC. Tested in Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

4°C and protect from prolonged exposure to light. Do not freeze.

Cite This Product

Anti-human CD14 Monoclonal Antibody APC Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00137-APC)

Host

Mouse

Contents

PBS, pH7.2, 0.09% NaN3 and 0.2% (w/v) BSA

Clonality

Monoclonal

Clone Number

26ic

Isotype

IgG2b,k

Immunogen

Cultured human peripheral blood monocytes

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

FC00137-APC is reactive to CD14 in Human

Applications

FC00137-APC is guaranteed for Flow Cytometry Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

User need to optimize the dilution ratio for this antibody

Validation Images & Assay Conditions

Gene/Protein Information For CD14 (Source: Uniprot.Org, NCBI)

Uniprot ID

P08571

Gene ID

929

Gene Name

CD14

Full Name

Monocyte differentiation antigen CD14

Weight

40.076kDa

Alternative Names

CD14 antigen; CD14 molecule; CD14; monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein CD14 CD14 molecule monocyte differentiation antigen CD14|myeloid cell-specific leucine-rich glycoprotein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CD14, check out the CD14 Infographic

CD14 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CD14: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

FC00137-APC has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Immunomodulatory activity of interleukin-27 in human chronic periapical diseases

Have you used Anti-human CD14 Monoclonal Antibody APC Conjugated, Flow Validated?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-human CD14 Monoclonal Antibody APC Conjugated, Flow Validated

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

11 Customer Q&As for Anti-human CD14 Monoclonal Antibody APC Conjugated, Flow Validated

Question

See below the WB image, lot number and protocol we used for lymphocyte using anti-human CD14 Monoclonal antibody APC Conjugated, Flow Validated FC00137-APC. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-03-27

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-27

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymphocyte using anti-human CD14 Monoclonal antibody APC Conjugated, Flow Validated FC00137-APC. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-03-02

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-02

Question

I was wanting to use your anti-human CD14 Monoclonal antibody APC Conjugated, Flow Validated for Flow Cytometry for human lymphocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lymphocyte identification?

C. Brown

Verified customer

Asked: 2020-02-19

Answer

As indicated on the product datasheet, FC00137-APC anti-human CD14 Monoclonal antibody APC Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human lymphocyte in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-02-19

Question

Is a blocking peptide available for product anti-human CD14 Monoclonal antibody APC Conjugated, Flow Validated (FC00137-APC)?

Verified Customer

Verified customer

Asked: 2019-09-06

Answer

We do provide the blocking peptide for product anti-human CD14 Monoclonal antibody APC Conjugated, Flow Validated (FC00137-APC). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-09-06

Question

Is there a BSA free version of anti-human CD14 Monoclonal antibody APC Conjugated, Flow Validated FC00137-APC available?

Verified Customer

Verified customer

Asked: 2018-06-27

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-human CD14 Monoclonal antibody APC Conjugated, Flow Validated FC00137-APC are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-06-27

Question

I see that the anti-human CD14 Monoclonal antibody APC Conjugated, Flow Validated FC00137-APC works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

B. Miller

Verified customer

Asked: 2018-05-25

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-05-25

Question

Will FC00137-APC anti-human CD14 Monoclonal antibody APC Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2017-12-20

Answer

It shows on the product datasheet, FC00137-APC anti-human CD14 Monoclonal antibody APC Conjugated, Flow Validated as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2017-12-20

Question

Does anti-human CD14 Monoclonal antibody APC Conjugated, Flow Validated FC00137-APC work for Flow Cytometry with lymphocyte?

K. Krishna

Verified customer

Asked: 2017-09-14

Answer

According to the expression profile of lymphocyte, CD14 is highly expressed in lymphocyte. So, it is likely that anti-human CD14 Monoclonal antibody APC Conjugated, Flow Validated FC00137-APC will work for Flow Cytometry with lymphocyte.

Boster Scientific Support

Answered: 2017-09-14

Question

Is this FC00137-APC anti-human CD14 Monoclonal antibody APC Conjugated, Flow Validated reactive to the isotypes of CD14?

J. Parker

Verified customer

Asked: 2017-02-27

Answer

The immunogen of FC00137-APC anti-human CD14 Monoclonal antibody APC Conjugated, Flow Validated is Cultured human peripheral blood monocytes. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-02-27

Question

My question regarding product FC00137-APC, anti-human CD14 Monoclonal antibody APC Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

S. Evans

Verified customer

Asked: 2017-02-23

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC00137-APC anti-human CD14 Monoclonal antibody APC Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-02-23

Question

We want to test anti-human CD14 Monoclonal antibody APC Conjugated, Flow Validated FC00137-APC on human lymphocyte for research purposes, then I may be interested in using anti-human CD14 Monoclonal antibody APC Conjugated, Flow Validated FC00137-APC for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

H. Collins

Verified customer

Asked: 2015-04-13

Answer

The products we sell, including anti-human CD14 Monoclonal antibody APC Conjugated, Flow Validated FC00137-APC, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2015-04-13

Size

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

In stock
Order Product
FC00137-APC
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$312.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.