Anti-Human CD54 Monoclonal Antibody, Flow Validated

Product Name

Anti-Human CD54 Monoclonal Antibody, Flow Validated

View all ICAM-1/CD54 Antibodies

SKU/Catalog Number

FC00171

Size

100ug, 500ug

Form

Liquid, in PBS, pH7.2, <0.09% NaN3 and 0.2% (w/v) BSA

Description

Boster Bio Anti-Human CD54 Monoclonal Antibody Unconjugated Conjugated, Flow Validated (Catalog# FC00171). Tested in Flow Cytometry application(s). This antibody reacts with Human.

Storage & Handling

Store at 2-8°C. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Human CD54 Monoclonal Antibody, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00171)

Host

Mouse

Contents

PBS pH 7.2, 0.1% (w/v) BSA, 0.09% (w/v) sodium azide

Clonality

Monoclonal

Clone Number

Clone: LB-2

Isotype

IgG2b,k

Immunogen

MHC Class II antigen negative human Blymphoblastoid cell line

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

FC00171 is reactive to ICAM1 in Human

Applications

FC00171 is guaranteed for Flow Cytometry Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

57.825kDa

Background of ICAM-1/CD54

CD45 (Cluster of Differentiation 45), also known as PTPRC, LCA or CD45R, is an enzyme that, in humans, is encoded by the PTPRC gene.CD45 is a member of the protein tyrosine phosphatase (PTP) family. CD45 is a major high molecular mass leukocyte cell surface molecule which is also an integral membrane protein tyrosine phosphatase.The cytogenetic location of CD45 is 1q31.3-q32.1. CD45 is especially a prototype for transmembrane protein-tyrosine phosphatase (PTP). Targeted disruption of the CD45 gene leads to enhanced cytokine and interferon receptor-mediated activation of JAKs and STAT proteins. In vitro, CD45 directly dephosphorylates and binds to JAKs. Functionally, CD45 negatively regulates interleukin-3-mediated cellular proliferation, erythropoietin-dependent hematopoiesis, and antiviral responses in vitro and in vivo. CD45 has been best studied in T cells, where it determines T cell receptor signaling thresholds. CD45 is moved into or out of the immunological synapse (IS) membrane microdomain depending on the relative influence of interaction with the extracellular galectin lattice or the intracellular actin cytoskeleton. Galectin interaction can be finetuned by varying usage of the heavily Oglycosylated spliced regions and sialylation of Nlinked carbohydrates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

User needs to optimize the dilution ratio for this antibody.

Validation Images & Assay Conditions

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Daudi gated cells stained with purified mouse anti-human CD54 (clone LB-2) followed by goat anti-mouse IgG (H+L) Alexa Fluor 488 staining (red histogram). Daudi gated cells stained with purified mouse IgG2b isotype control followed by goat anti-mouse IgG (H+L) Alexa Fluor 488 staining (Blue histogram).

Gene/Protein Information For ICAM1 (Source: Uniprot.org, NCBI)

Alternative Names

BB2; CD54 antigen; CD54; cell surface glycoprotein P3.58; human rhinovirus receptor; ICAM1; ICAM-1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule 1; Major group rhinovirus receptor; P3.58 ICAM1 BB2, CD54, P3.58 intercellular adhesion molecule 1 intercellular adhesion molecule 1|ICAM-1|cell surface glycoprotein P3.58|epididymis secretory sperm binding protein|intercellular adhesion molecule 1 (CD54), human rhinovirus receptor|major group rhinovirus receptor

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