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Product Info Summary
| SKU: | A01635-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-IL-16 Antibody
SKU/Catalog Number
A01635-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-IL-16 Antibody (Catalog # A01635-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
IL-16 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-IL-16 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01635-1)
Host
Rabbit
Contents
IL-16 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
IL-16 antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of human IL-16. The immunogen is located within the first 50 amino acids of IL-16.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody will only detect the NIL-16 isoform.
Reactive Species
A01635-1 is reactive to IL16 in Human, Mouse, Rat
Applications
A01635-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
141.752kDa
Background of IL-16
IL-16 was initially identified as a chemotactic cytokine, but is now known to possess a wide range of activities. Later studies have more fully characterized IL-16 as an immunomodulatory cytokine that contributes to the regulatory process of CD4+ T cell recruitment and activation at sites of inflammation in association with asthma and several autoimmune diseases. The precursor of IL-16 (pro-IL-16) is thought to be cleaved towards the C-terminal region by Caspase-3, releasing a 20 kDa active form that binds to and signals through CD4. Besides acting as a chemotactic cytokine, IL-16 is thought to also be involved in the regulation of T cell proliferation and multiple infectious, immune-mediated, and autoimmune inflammatory disorders including irritable bowel syndrome, systemic lupus erythematosus, and neurodegenerative disorders. At least two isoforms of IL-16 are known to exist; the longer isoform (also known as NIL-16) is detected only in neurons of the cerebellum and hippocampus.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IL-16 antibody can be used for detection of IL-16 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of IL-16 in rat brain tissue lysate with IL-16 antibody at (A) 1 μg/ml and (B) 2 μg/mL.
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Immunohistochemistry of IL-16 in mouse brain tissue with IL-16 antibody at 2.5 μg/mL.
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Immunofluorescence of IL-16 in mouse brain tissue with IL-16 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For IL16 (Source: Uniprot.org, NCBI)
Gene Name
IL16
Full Name
Pro-interleukin-16
Weight
141.752kDa
Alternative Names
FLJ16806; FLJ42735; FLJ44234; HsT19289; IL16; IL-16; interleukin 16 (lymphocyte chemoattractant factor); LCF; LCFprIL-16; lymphocyte chemoattractant factor; neuronal interleukin 16; NIL16; PRIL16; prointerleukin 16; pro-interleukin-16 IL16 LCF, NIL16, PRIL16, prIL-16 interleukin 16 pro-interleukin-16|lymphocyte chemoattractant factor|neuronal interleukin 16|prointerleukin 16
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL16, check out the IL16 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL16: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL-16 Antibody (A01635-1)
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