Click image to see more details
Product Info Summary
| SKU: | A00113-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-IL33 Antibody Picoband™
SKU/Catalog Number
A00113-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL33 Antibody Picoband™ catalog # A00113-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL33 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00113-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse IL33 recombinant protein (Position: A102-I266).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00113-1 is reactive to Il33 in Mouse, Rat
Applications
A00113-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
31 kDa
Calculated molecular weight
29.991kDa
Background of IL-33
Interleukin 33 (IL-33) is a protein that in humans is encoded by the IL33 gene. The protein encoded by this gene is a cytokine that binds to the IL1RL1/ST2 receptor. The encoded protein is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. Several transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
ELISA (Cap), 1-5μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IL33 using anti-IL33 antibody (A00113-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates,
Lane 2: rat NRK whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL33 antigen affinity purified polyclonal antibody (Catalog # A00113-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL33 at approximately 31KD. The expected band size for IL33 is at 31KD.
Protein Target Info & Infographic
Gene/Protein Information For Il33 (Source: Uniprot.org, NCBI)
Gene Name
Il33
Full Name
Interleukin-33
Weight
29.991kDa
Superfamily
IL-1 family
Alternative Names
C9orf26; C9orf26chromosome 9 open reading frame 26 (NF-HEV); DKFZp586H0523; DVS27; DVS27-related protein; IL1F11; IL-1F11; IL33; IL-33; interleukin 33; Interleukin-1 family member 11; interleukin-33; NFHEV; NF-HEV; NF-HEVNFEHEV; Nuclear factor from high endothelial venules; RP11-575C20.2 Il33|9230117N10Rik, Il-, Il-33, Il1f, Il1f11, NF-H, NF-HEV|interleukin 33|interleukin-33|nuclear factor from high endothelial venules
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Il33, check out the Il33 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Il33: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL33 Antibody Picoband™ (A00113-1)
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No publications found for A00113-1
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Recommended Resources
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-IL33 Antibody Picoband™
Question
We ordered your anti-IL33 antibody for WB on left coronary artery last year. I am using mouse, and I plan to use the antibody for IHC-P next. We need examining left coronary artery as well as adrenal gland trachea in our next experiment. Do you have any suggestion on which antibody would work the best for IHC-P?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
I looked at the website and datasheets of our anti-IL33 antibody and it seems that A00113-1 has been tested on mouse in both WB and IHC-P. Thus A00113-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-19
Question
Is there a BSA free version of anti-IL33 antibody A00113-1 available?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-IL33 antibody A00113-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-10
Question
Will anti-IL33 antibody A00113-1 work for IHC-P with left coronary artery?
Verified Customer
Verified customer
Asked: 2019-09-02
Answer
According to the expression profile of left coronary artery, IL33 is highly expressed in left coronary artery. So, it is likely that anti-IL33 antibody A00113-1 will work for IHC-P with left coronary artery.
Boster Scientific Support
Answered: 2019-09-02
Question
Will A00113-1 anti-IL33 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
As indicated on the product datasheet, A00113-1 anti-IL33 antibody as been tested on IHC-P. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-12
Question
Is this A00113-1 anti-IL33 antibody reactive to the isotypes of IL33?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
The immunogen of A00113-1 anti-IL33 antibody is E. coli-derived mouse IL33 recombinant protein (Position: A102-I266). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-15
Question
Are we able to have Anti-IL33 Picoband Antibody A00113-1 in a BSA free format? Are there extra costs? What is the lead time?
Verified Customer
Verified customer
Asked: 2019-06-10
Answer
We have BSA free
Boster Scientific Support
Answered: 2019-06-10
Question
Please see the WB image, lot number and protocol we used for left coronary artery using anti-IL33 antibody A00113-1. Please let me know if you require anything else.
L. Jackson
Verified customer
Asked: 2019-02-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-02-12
Question
We have seen staining in rat adrenal gland trachea. Any tips? Is anti-IL33 antibody supposed to stain adrenal gland trachea positively?
Verified Customer
Verified customer
Asked: 2018-12-28
Answer
From what I have seen in literature adrenal gland trachea does express IL33. From what I have seen in Uniprot.org, IL33 is expressed in left coronary artery, endothelial cell, adrenal gland trachea, brain, among other tissues. Regarding which tissues have IL33 expression, here are a few articles citing expression in various tissues:
Adrenal gland, and Trachea, Pubmed ID: 14702039
Brain, Pubmed ID: 15489334
Endothelial cell, Pubmed ID: 12819012
Boster Scientific Support
Answered: 2018-12-28
Question
I was wanting to use your anti-IL33 antibody for IHC-P for rat left coronary artery on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat left coronary artery identification?
Verified Customer
Verified customer
Asked: 2018-08-15
Answer
It shows on the product datasheet, A00113-1 anti-IL33 antibody has been validated for ELISA, IHC-P, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat left coronary artery in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-15
Question
I see that the anti-IL33 antibody A00113-1 works with IHC-P, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
You can find protocols for IHC-P on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-08-02
Question
My lab would like to test anti-IL33 antibody A00113-1 on rat left coronary artery for research purposes, then I may be interested in using anti-IL33 antibody A00113-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-06-14
Answer
The products we sell, including anti-IL33 antibody A00113-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-06-14
Question
My team were well pleased with the WB result of your anti-IL33 antibody. However we have observed positive staining in left coronary artery nucleus using this antibody. Is that expected? Could you tell me where is IL33 supposed to be expressed?
T. Jones
Verified customer
Asked: 2018-02-13
Answer
Based on literature, left coronary artery does express IL33. Generally IL33 expresses in nucleus. Regarding which tissues have IL33 expression, here are a few articles citing expression in various tissues:
Adrenal gland, and Trachea, Pubmed ID: 14702039
Brain, Pubmed ID: 15489334
Endothelial cell, Pubmed ID: 12819012
Boster Scientific Support
Answered: 2018-02-13
Question
I have a question about product A00113-1, anti-IL33 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-07-06
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00113-1 anti-IL33 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-07-06
Question
Is a blocking peptide available for product anti-IL33 antibody (A00113-1)?
P. Huang
Verified customer
Asked: 2016-10-26
Answer
We do provide the blocking peptide for product anti-IL33 antibody (A00113-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-10-26
Question
We are currently using anti-IL33 antibody A00113-1 for mouse tissue, and we are well pleased with the ELISA results. The species of reactivity given in the datasheet says mouse, rat. Is it true that the antibody can work on primate tissues as well?
K. Wu
Verified customer
Asked: 2016-02-10
Answer
The anti-IL33 antibody (A00113-1) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-02-10
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for left coronary artery using anti-IL33 antibody A00113-1. Let me know if you need anything else.
H. Williams
Verified customer
Asked: 2015-07-23
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-07-23
