Anti-KRT7 Antibody Picoband™

Product Name

Anti-KRT7 Antibody Picoband™

View all KRT7 Antibodies

SKU/Catalog Number

A02416-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-KRT7 Antibody Picoband™ catalog # A02416-2. Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-KRT7 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02416-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human KRT7, which shares 91.3% amino acid (aa) sequence identity with mouse and rat KRT7.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A02416-2 is reactive to KRT7 in Human

Applications

A02416-2 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

51 kDa

Calculated molecular weight

51.386kDa

Background of KRT7

Keratin, type II cytoskeletal 7 also known as cytokeratin-7 (CK-7) or keratin-7 (K7) or sarcolectin (SCL) is a protein that in humans is encoded by the KRT7 gene. The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of KRT7 using anti-KRT7 antibody (A02416-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human RT4 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT7 antigen affinity purified polyclonal antibody (Catalog # A02416-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KRT7 at approximately 51 kDa. The expected band size for KRT7 is at 51 kDa.

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Figure 2. IHC analysis of KRT7 using anti-KRT7 antibody (A02416-2).
KRT7 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KRT7 Antibody (A02416-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 3. IHC analysis of KRT7 using anti-KRT7 antibody (A02416-2).
KRT7 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KRT7 Antibody (A02416-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

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Figure 4. IHC analysis of KRT7 using anti-KRT7 antibody (A02416-2).
KRT7 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-KRT7 Antibody (A02416-2) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.

Gene/Protein Information For KRT7 (Source: Uniprot.org, NCBI)

Alternative Names

CK7; CK-7; cytokeratin 7; cytokeratin-7; K2C7; K7keratin, 55K type II cytoskeletal; keratin 7; keratin, type II cytoskeletal 7; keratin-7; MGC129731; MGC3625; sarcolectin; SCLkeratin, simple epithelial type I, K7; type II mesothelial keratin K7; Type-II keratin Kb7 KRT7 CK7, K2C7, K7, SCL keratin 7 keratin, type II cytoskeletal 7|CK-7|cytokeratin 7|keratin 7, type II|keratin, 55K type II cytoskeletal|keratin, simple epithelial type I, K7|sarcolectin|type II mesothelial keratin K7|type-II keratin Kb7

For more info on KRT7, check out the KRT7 Infographic

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In this infographic, you will see the following information for KRT7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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