Anti-Methionine aminopeptidase 2 METAP2 Antibody

Product Name

Anti-Methionine aminopeptidase 2 METAP2 Antibody

View all Methionine Aminopeptidase 2/METAP2 Antibodies

SKU/Catalog Number

A03648-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Methionine aminopeptidase 2 METAP2 Antibody catalog # A03648-1. Tested in WB,ICC,IF,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Methionine aminopeptidase 2 METAP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03648-1)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from human MYO10 protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03648-1 is reactive to METAP2 in Human, Mouse, Rat

Applications

A03648-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

52.892kDa

Background of Methionine Aminopeptidase 2/METAP2

Pro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.

Hongzhe Li, J. Biol. Chem., Feb 2007; 282: 4943 - 4950.
Wuhan Xiao, Cancer Res., Aug 2003; 63: 4698 - 4704.
Thierry Virolle, J. Biol. Chem., Mar 2003; 278: 11802 - 11810.
Derek J. Richard, Genes & Dev., Feb 2001; 15: 328.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-1:2,000
ICC/IF: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100

Validation Images & Assay Conditions

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Figure 2. Immunocytochemistry staining of METAP2 using Anti-Methionine aminopeptidase 2 METAP2 Antibody (A03648-1).

ICC staining Methionine Aminopeptidase 2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

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Figure 1. Western blotting validation for Anti-Methionine aminopeptidase 2 METAP2 Antibody A03648-1

Western blot analysis of Methionine Aminopeptidase 2 on Hela cell using anti-Methionine Aminopeptidase 2 antibody at 1/1

Gene/Protein Information For METAP2 (Source: Uniprot.org, NCBI)

Alternative Names

Amp2; eIF-2-associated p67 homolog; Initiation factor 2-associated 67 kDa glycoprotein; MAP 2; MAP2; MetAP 2; METAP2; Methionine Aminopeptidase 2; methionyl aminopeptidase 2; MNPEP; p67; p67EC 3.4.11.18; p67eIF2; Peptidase M 2 METAP2 MAP2, MNPEP, p67eIF2 methionyl aminopeptidase 2 methionine aminopeptidase 2|eIF-2-associated p67 homolog|initiation factor 2-associated 67 kDa glycoprotein|peptidase M 2|testicular tissue protein Li 17

For more info on METAP2, check out the METAP2 Infographic

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In this infographic, you will see the following information for METAP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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